Chondroitinase ABC treatment of injured spinal cord in rats Evaluation of long-term outcomes

作     者：Haifeng Yuan Yongli Ding Yueming Song Lihong Hu Zili Wang Hao Liu Limin Liu Quan Gong Tao Li Qingquan Kong 

作者机构：Department of Orthopedic Surgery West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Orthopedic Surgery Affiliated Hospital Ningxia Medical University Yinchuan 750004 Ningxia Hui Autonomous Region China Department of Orthopedic Surgery First Affiliated Hospital Henan Chinese Medical College Zhengzhou 450000 Henan Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2010年第5卷第16期

页      面：1238-1242页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：the National Natural Science Foundation of China No.30471759 

主　　题：spinal cord injury chondroitinase ABC morphology treatment biotinylated dextran amine anterograde tracing neural regeneration 

摘      要：Chondroitin sulfate proteoglycans （CSPGs） which are produced by mature oligodendrocytes and reactive astrocytes can be upregulated after spinal cord injury and contribute to regenerative failure. Chondroitinase ABC （ChABC） digests glycosaminoglycan chains on CSPGs and can thereby overcome CSPG-mediated inhibition. However, many current studies have used an incomplete spinal cord injury model, and examined results after 8-12 weeks of ChABC treatment. In this study, a complete rat spinal cord transection injury model was used to study the long-term effects of ChABC treatment by subarachnoid catheter. Pathology of spinal cord regeneration was compared with control 24 weeks following ChABC treatment using immunohistochemistry and axon tracing techniques. At 24 weeks after injury, neurofilament 200 expression was significantly greater in the ChABC treatment group compared with the transection group. In the ChABC treatment group, axonal growth was demonstrated by a large number of biotinylated dextran amine positive axons caudal to, or past, the epicenter of injury. Biotinylated dextran amine-labeled fibers were found in the proximal end of the spinal cord in the transection alone group. These results confirm that ChABC can promote axon growth, neural regeneration, and repair after spinal cord injury in rats long after the initial injury.