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Differential regulation of glutathione S-transferase Yb1mRNA levels in rat prostate, liver and brain by androgen

Cell Research 1995年第2期5卷 235-242页

作者： ZHANG YUAN YOUDUAN ZHANG CHAWNSHANG CHANG YONGLIAN ZHANG(Shanghai Institute of Biochemistry, Academia Sinica, 320Yueyang Road, Shanghai 200031, China.)(Department of Human Oncology, University of Wisconsin, 600 Highland Avenue, Madison, WI 53792, USA.) Shanghai Institute of Biochemistry Academia Sinica Shanghai China Department of Human Oncology University of Wiscon-sin USA

Northern blot analysis of glutathione S-transferase (GST) Yb1 mRNA in different tissues of male and female rats revealed that its tissue-specific transcription patterns were highly sex hormone related. Although the GST Yb1 mRNA could be detected in most of the tissues examined at various levels, the highest abundance was observed in the ventral prostate, uterus and liver, which were the main target tissue for androgen, estrogen and glucocorticoid respectively The effect of androgen on the transcription of GST Yb1 was also tissue-specific. Since androgen withdrawal by castration caused the up-regulation of GST Yb1 mRNA in the ventral prostate but down-regulation in the liver and no effect in the brain, evaluation of this system for studying the regulation mechanisms of gene expression by which androgen exerts its differential effects has been discussed.

关键词： GST Yb1 mRNA androgen regulation rat prostate rat liver rat brain

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Glutathione peroxidase activity in cell cultures from different regions of human epididymis

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Asian Journal of Andrology 2005年第1期7卷 33-37,页

作者： Enrique Castellon Hernan Rioseco Juan Rojas Michel Royer Eduardo Salas Hoctor Contreras Christian Huidobro Physiology and Biophysics Program Institute of Biomedical Sciences Faculty of Medicine University. of Chile Santiago 6530499 Chile Urology Department Clinic Hospital J. J. Aguirre University of Chile Santiago 6530499 Chile

Aim: To study the secretory activity and androgen regulation of glutathione peroxidase (GPx) in epithelial cell cultures from human epididymis. Methods: Tissue was obtained from patients undergoing therapeutic orchidectomy for prostatic cancer. Epithelial cell cultures were obtained from the caput, corpus and cauda epididymides. Enzymatic activity was measured in conditioned media by colorimetric methods in absence or presence of 1, 10 or 100 nrnoI.L^(-1) testosterone. The effect of 1 μmol.L^(-1) flutamide was also evaluated. Results: GPx activity was higher in cultures from corpus and cauda than caput epididymidis. The presence of different concentrations of testosterone increase enzyme activity in cell cultures from all epididymal regions. Addition of flutamide reverses the androgen dependent increase of GPx activity. Conclusion: GPx activity is secreted from human epididymal cells in a region dependent manner and is regulated by androgens.

关键词： glutathione peroxidase human epididymis cell culture androgen regulation

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