检索结果-南通市图书馆

检索条件"主题词=alp-miR066"

共 2 条记录，以下是1-10 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

Identification of a novel mirNA important for melanogenesis in alpaca(Lama Pacos)

Identification of a novel miRNA important for melanogenesis ...

引用

提升骆驼产业科技含量，打造骆驼行业知名品牌——第二届（2013）中国骆驼大会

作者： Ruiwen Fan Shanshan Yang Zhanquan Shi Kaiyuan Ji Xiaoyun Jia Jianbo Yao George W Smith Changsheng Dong

The mechanism of coat color formation is complex,microRNAs play important roles in the control of melanogenesis and coat color in *** the previous deep sequencing of alpaca skins,one of novel mirNAs expressed differently in white and black alpaca skin(named alp-mir066).According to the target gene prediction,one of the target genes is soluble guanylate cyclase(sGC).To get the function of alp-mir-066 in alpaca skin,it was over expressed in alpaca melanocytes in *** expression of mirNA-066 was tested by real-time PCR and the results showed it expressed *** the pathway of cAMP in melanocytes,cGMP was increased by ELASA assay,while cAMP was decreased. The expression of sGC,TYR,TYRP1,TYRP2 and MITF protein were tested by western blotting,and the results showed that sGC was increased and TYR,TYRP1 and MITF protein were ***,the number of melanogenic melanocytes was less and the amount of melanins production was 0.7 fold in alp-mir066 transfection group,compared to negative control(NC) transfection *** those results suggested that alp-mir-066 might regulate melanogenesis on the pathway of cAMP in alpaca melanocytes by targeting sGC.

关键词： alp-mir066 melanocytes cAMP melanin sGC

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification of a Novel mirNA in Melanocytes of alpaca(Lama Pacos)

Identification of a Novel miRNA in Melanocytes of Alpaca(Lam...

引用

中国畜牧兽医学会动物解剖及组织胚胎学分会第十八次学术研讨会

作者： Ruiwen Fan Shanshan Yang Zhanquan Shi Kaiyuan Ji Xiaoyun Jia Jianbo Yao George W Smith Changsheng Dong College of Animal Science and Technology Shanxi Agricultural University Laboratory of Animal Biotechnology and Genomics Division of Animal and Nutritional SciencesWest Virginia University Laboratory of Mammalian Reproductive Biology and Genomics Departments of Animal Science and PhysiologyMichigan State University

From the previous deep sequencing of alpaca skins,one of novel mirNAs expressed differently in white and black alpaca skin(named alp-mir066).According to the target gene prediction,one of the target genes is soluble guanylate cyclase(sGC).To get the function of alp-mir-066 in alpaca skin,it was over expressed in alpaca melanocytes in *** expression of mirNA-066 was tested by real-time PCR and the results showed it expressed *** the pathway of cAMP in melanocytes,cGMP was increased by ELASA assay,while cAMP was *** expression of sGC,TYR,TYRP1,TYRP2 and MITF protein were tested by western blotting,and the results showed that sGC was increased and TYR,TYRP1 and MITF protein were ***,the number of melanogenic melanocytes was less and the amount of melanins production was 0.7 fold in alp-mir066 transfection group,compared to Negative control(NC) transfection *** those results suggested that alp-mir-066 might regulate melanogenesis on the pathway of cAMP in alpaca melanocytes by targeting sGC.

关键词： alp-mir066 melanocytes cAMP melanin sGC

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

全选清除本页清除全部题录导出标记到“检索档案”

共1页 << < 1 > >>

回到顶部

执行限定条件

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：