Identification of a novel miRNA important for melanogenesis in alpaca(Lama Pacos)
会议名称：《提升骆驼产业科技含量，打造骆驼行业知名品牌——第二届（2013）中国骆驼大会》
会议日期：2013年
关 键 词：alp-miR066 melanocytes cAMP melanin sGC
摘 要：The mechanism of coat color formation is complex,microRNAs play important roles in the control of melanogenesis and coat color in *** the previous deep sequencing of alpaca skins,one of novel miRNAs expressed differently in white and black alpaca skin(named alp-miR066).According to the target gene prediction,one of the target genes is soluble guanylate cyclase(sGC).To get the function of alp-miR-066 in alpaca skin,it was over expressed in alpaca melanocytes in *** expression of miRNA-066 was tested by real-time PCR and the results showed it expressed *** the pathway of cAMP in melanocytes,cGMP was increased by ELASA assay,while cAMP was decreased. The expression of sGC,TYR,TYRP1,TYRP2 and MITF protein were tested by western blotting,and the results showed that sGC was increased and TYR,TYRP1 and MITF protein were ***,the number of melanogenic melanocytes was less and the amount of melanins production was 0.7 fold in alp-miR066 transfection group,compared to negative control(NC) transfection *** those results suggested that alp-miR-066 might regulate melanogenesis on the pathway of cAMP in alpaca melanocytes by targeting sGC.