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alcohol dehydrogenase 5 of Helicoverpa armigera interacts with the CYP6B6 promoter in response to 2-tridecanone

Insect Science 2020年第5期27卷 1053-1066页

作者： Jie Zhao Qian Wei Xin-Rong Gu Su-Wei Ren Xiao-Ning Liu Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Sciences and TechnologyXinjiang UniversityUrumqiXinjiangChina Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization College of AgricultureShihezi UniversityShiheziXinjiangChina

alcohol dehydrogenase 5(ADH5)is a member of medium-chain dehydro-genase/reductase family and takes part in cellular formaldehyde and S-nitrosoglutathione metabolic network.2-tridecanone(2-TD)is a toxic compound in many Solanaceae crops to defend against a variety of herbivory insects.In the broader context of insect development and pest control strategies,this study investigates how a new ADHS from Helicoverpa armigera(HaADH5)regulates the expression of CYP6B6,a gene involved in molting and metamorphosis,in response to 2-TD treatment.Cloning of the HaADH5 complementary DNA sequence revealed that its 1002 bp open reading frame encodes 334 amino acids with a predicted molecular weight of 36.5 kD.HaADH5 protein was purified in the Es-cherichia coli Transetta(pET32a-HaADH5)strain using a prokaryotic expression system.The ability of HaADH5 protein to interact with the 2-TD responsive region within the promoter of CYP6B6 was confirmed by an in vitro electrophoretic mobility shift assay and transcription activity validation in yeast.Finally,the expression levels of both HaADH5 and CYP6B6 were found to be significantly decreased in the midgut of 6th instar larvae after 48 h of treatment with 10 mg/g 2-TD artificial diet.These results indicate that upon 2-TD treatment of cotton bollworm,HaADHS regulates the expression of CYP6B6 by interacting with its promoter.As HaADH5 regulation of CYP6B6 expression may con-tribute to the larval xenobiotic detoxification,molting and metamorphosis,HaADH5 is a.candidate target for controlling the growth and development of cotton bollworm.

关键词： alcohol dehydrogenase DNA-protein interaction Helicoverpa armigera regulatory factor 2-tridecanone

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alcohol dehydrogenase Activity in Cultured Cells from Different Tobacco (Nicotiana tabacum L.) Varieties

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Wuhan University Journal of Natural Sciences 1999年第4期4卷 495-497页

作者： ZHENG Ling, YANG Yue sheng ,ZHENG Ying dong College of Biotechnology, South China Agricultural University, Guangzhou 510642,China

The activity of alcohol dehydrogenase (ADH) in cultured cells of various tobacco was determined. It was found that significant differences existed in cells of different varieties cultured under normal conditions and as well after treated with exogenous ethanol. The ADH activity had positive relation with the ability of the cells to catabolize exogenous ethanol, indicating that the main function of the ADH in tobacco cells was in the direction of converting ethanol to acetaldehyde.

关键词： alcohol dehydrogenase suspension cultured cells tobacco

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Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

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Journal of Integrative Agriculture 2017年第8期16卷 1742-1750页

作者： QIN Gai-hua QI Xiao-xiao QI Yong-jie GAO Zheng-hui YI Xing-kai PAN Hai-fa XU Yi-liu Horticultural Research Institute Anhui Academy of Agricultural SciencesHefei 230031P.R.China Anhui Key Laboratory of Genetic Improvement and Ecophysiology of Horticultural Crop Hefei 230031P.R.China College of Agriculture Medicine and HealthAnhui Radio and Television UniversityHefei 230022P.R.China

alcohol dehydrogenase （ADH） catalyzes the interconversion of aldehydes and their corresponding alcohols, and is a key enzyme in volatile ester biosynthesis. However, little is known regarding ADH and ADH encoding genes （ADHs） in pear. We identified 8 ADHs in the pear＇s genome （PbrADHs） by multiple sequences alignment. The PbrADHs were highly ho- mologous in their coding regions, while were diversiform in structure. 9 introns were predicted in PbrADH3-PbrADH8, while 8 introns, generated through exon fusion and intron loss, were predicted in PbrADH1 and PbrADH2. To study the genetic regulation underlying aroma biogenesis in pear fruit, we determined the PbrADH transcripts, ADH activities and volatile contents of fruits during ripening stage for Nanguoli and Dangshansuli, two cultivars having different aroma characteristics. ADH activity was strongly associated with the transcription of ADH~ in the two cultivars during fruit ripening stage. The higher ester content paralleling to a higher ADH activity was detected in Nanguoli than in Dangshansuli, so it is induced that the lower ester content in Dangshansuli fruit may be the result of weak ADH activity. The present study revealed that total ADH activity and volatile ester production correlated with increased PbrADHstranscript levels. PbrADH6 may contribute to ADH activity catalyzing aldehyde reduction and ester formation in pear fruit.

关键词： alcohol dehydrogenase ester volatile gene expression pear

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Achieving Enzyme Stability Using a Simple Fabrication Procedure： The alcohol dehydrogenase Example

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Journal of Pharmacy and Pharmacology 2018年第2期6卷 188-196页

作者： I. Ribau E. Fortunato Departamento de Ciencias dos materiais Faculdade de Ciencias and Tecnologia Universidade Nova de Lisboa Campus de Caparica Caparica 2829-516 Portugal

The use of screen-printing biosensors has been updated in this article as a tool to analyze the electron transfer process involving redox proteins or enzymes. The aim of this research was to fabricate a simple apparatus which allowed the use of the enzymes (in the solid state) to maintain their stability. To prove this concept an enzyme in the solid state was mixed with the carbon ink and this mixture was used to print the working electrode. We choose as proving the alcohol dehydrogenase. The first reason is because it metabolizes the alcohol, which can be present in biological samples of blood, saliva and urine and also in the beverage; the second is that this enzyme is still a challenge to electrochemistry due to having lower stability in sensors. The results show that in this device the enzyme was active and stable during all the experiments and in the experimental conditions that could catalyze the ethanol to acetaldehyde. These devices have the advantage of being disposable, cheap and are easy to fabricate. And also, they do not need expensive tools to be fabricated, they only need 2 µL of electrolyte or sample, and they need lower amounts of enzyme to permit electrochemical studies.

关键词： Paper biosensor disposable alcohol dehydrogenase multiple use

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Association of alcohol dehydrogenase and aldehyde dehydrogenase 2 genetic polymorphisms with serum lipid profile: meta-analysis of mendelian randomisation studies

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South China Journal of Cardiology 2014年第4期15卷 238-250,256页

作者： GAO Jia-jia JIN Dong-mei WEN Zhu-zhi IP Chi-kin WANG Jing-feng GENG Deng-feng Department of Cardiology Sun Yat-sen Memorial Hospital Sun Yat-sen University Department of Rehabilitation Medicine Sun Yat-sen Memorial Hospital Sun Yat-sen University

Background alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic polymorphisms and serum lipid profile （SLP）, however, the results were inconsistent. Methods Fourteen articles involving 27,917 participants were included in this meta-analysis. Weighted mean difference （WMD） and 95% confidence intervals （CIs） were presented using random effects model. Publication bias was evaluated by funnel plot and Begg＇s test. In addition, to further explore the heterogeneity, subgroup analysis were performed. Results Overall, there was no association between ADH genetic polymorphisms and SLP with no regard for drinking status. However, compared with ALDH2 wild homozygous genotype, ALDH2 mutant genotypes were associated with significant decrease in serum high density lipoprotein cholesterol （HDL- C）（WMD -1.80 mg/dL, 95% CI -1.88 to -1.72, P 〈 0.001） and total cholesterol （TC） levels （WMD -1.]0 mg/dL, 95% CI -1.59 to -0.62, P 〈 0.001）, and significant increase in serum low density lipoprotein choles- terol （LDL-C） level （WMD 0.30 mg/dL, 95% CI 0.18 to 0.43, P 〈 0.001）. Although there was no significant difference in serum triglyceride level in the overall population, subgroup analysis revealed that compared with ALDH2 ＊ 1 wild homozygote, ALDH2 ＊ 2 allele displayed a significant difference in serum triglyceride level between the female and male （female： WMD 1.69 mg/dl, 95% CI 1.08 to 2.30, P 〈 0.001; male： WMD -6.42 mg/dL, 95% CI -12.15 to -0.68, P = 0.028）. Conclusion ADH genetic polymorphism has no association with SLP, regardless of sex category and drinking status. ALDH2 genetic polymorphism has slight association with HDL-C, LDL-C and TC levels and sex-specific association with serum triglyceride level. Whether or not the association between ADH2 genetic polymorphisms and SLP is resulted from alcohol con-sumption needs further investigation.

关键词： alcohol dehydrogenase aldehyde dehydrogenase serum lipids meta-analysis

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Positive effect of ethanol-induced Lactococcus lactis on alcohol metabolism in mice

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Food Science and Human Wellness 2022年第5期11卷 1183-1190页

作者： Sisi Chen Shimin Jia Keke Suo Qiaozhen Kang Limin Hao Laizheng Lu Xin Liu Jinyong Huang Jike Lu School of Life Sciences Zhengzhou UniversityZhengzhou 450001China Physical Education College Zhengzhou UniversityZhengzhou 450001China The Quartermaster Research Institute of Engineering and Technology Academy of Military Sciences PLA ChinaBeijing 100010China Zhengzhou Mindtek Biotechnology Co. Ltd.Zhengzhou 450001China School of Agricultural Sciences Zhengzhou University 450001China

It is well known that exposure to environmental stresses could enhance the adaptability of bacteria and up-regulate the expression of a variety of oxidative stress-related genes and antioxidant enzymes.It is unclear whether the adaptability of microorganisms formed naturally in special environments could transfer to other organisms.The study aimed to evaluate the effects of untreated and ethanol-induced Lactococcus lactis intracellular extracts(U-IE and E-IE)on alcohol metabolism in mice.The positive effects of E-IE on alcohol metabolism in mice were revealed by the enhanced latency of loss of righting reflex(LORR),the reduced duration of LORR,the decrease of blood alcohol concentration,as well as the elevation of alcohol dehydrogenase(ADH)activities in the stomach and liver tissues.Furthermore,the potential benefits of E-IE on the liver were evaluated by biochemical parameters including the activities of serum transaminase,the levels of antioxidant enzymes,and the pathological changes of liver tissue.The present work put forward a new point that appropriate ethanol stress could enhance the intracellular ADH activity of L.lactis,and its intracellular extracts could continue to enhance alcohol metabolism in mice.

关键词： Ethanol stress Intracellular extracts alcohol dehydrogenase alcohol metabolism

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Meta-analysis reveals up-regulation of cholesterol processes in non-alcoholic and down-regulation in alcoholic fatty liver disease

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World Journal of Hepatology 2017年第8期9卷 443-454页

作者： Wasco Wruck James Adjaye Medical Faculty Institute for Stem Cell Research and Regenerative MedicineHeinrich Heine University40225 DusseldorfGermany

AIM To compare transcriptomes of non-alcoholic fatty liver disease(NAFLD) and alcoholic liver disease(ALD) in a meta-analysis of liver biopsies.METHODS Employing transcriptome data from patient liver biopsies retrieved from several public repositories we performed a meta-analysis comparing ALD and NAFLD.RESULTS We observed predominating commonalities at the transcriptome level between ALD and NAFLD,most prominently numerous down-regulated metabolic pathways and cytochrome-related pathways and a few up-regulated pathways which include ECM-receptor interaction,phagosome and lysosome.However some pathways were regulated in opposite directions in ALD and NAFLD,for example,glycolysis was down-regulated in ALD and up-regulated in NAFLD.Interestingly,we found rate-limiting genes such as HMGCR,SQLE and CYP7A1 which are associated with cholesterol processes adversely regulated between ALD(down-regulated) and NAFLD(up-regulated).We propose that similar phenotypes in both diseases may be due to a lower level of the enzyme CYP7A1 compared to the cholesterol synthesis enzymes HMGCR and SQLE.Additionally,we provide a compendium of comparative KEGG pathways regulation in ALD and NAFLD.CONCLUSION Our finding of adversely regulated cholesterol processes in ALD and NAFLD draws the focus to regulation of cholesterol secretion into bile.Thus,it will be interesting to further investigate CYP7A1-mediated cholesterol secretion into bile-also as possible drug targets.The list of potential novel biomarkers may assist differential diagnosis of ALD and NAFLD.

关键词： Non-alcoholic fatty liver disease alcoholic liver disease cholesterol Bile alcohol dehydrogenase CYP7A1

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THE POSSIBLE GENETIC BACKGROUND OF alcohol-INDUCED PANCREATIC STEATOSIS

THE POSSIBLE GENETIC BACKGROUND OF ALCOHOL-INDUCED PANCREATI...

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第十七届西南地区消化病学术会议暨2014贵州省消化病及...

作者： Jing Li Yi Xie Fang Yuan Bin Song Chengwei Tang Department of Gastroenterology West China HospitalSichuan University Division of Peptides Related with Human Diseases Key Laboratory of Biotherapy of Human DiseasesMinistry of Education Department of Radiology West China HospitalSichuan University

Background and objective:AIcohol-induced pancreatic steatosis is associated with alcohol metabolism in the pancreas,in which alcohol dehydrogenase(ADH)and aldehyde dehydrogenase(ALDH)are the major oxidative enzyme.And genetic polymorphism of ADH2 and ALDH2 is known to be closely related to alcohol dependence.But it is still unknown whether genetic polymorphism of them is also relevant to pancreatic steatosis in alcoholics.This study is to find out the possible genetic background of alcohol-induced pancreatic steatosis in alcoholics by analyzing genetic polymorphism in ADH2 and ALDH2.Methods:163 alcoholic male aged 20-70 years with a normal body mass index were recruited into this study.The alcoholics were defined as the drinkers with an alcohol intake of>80 g/day,a duration of>5 years,and abstinence from alcohol within 2 years.They received magnetic resonance scanning in the epigastric region by using double-echo chemical shift magnetic resonance technique.PCR-restriction fragment length polymorphism was used for ADH2 and ALDH2 genotype detection.The drinkers with pancreatic fat content higher than 4.2%,8.0%in alcoholics younger and older than 50 years respectively were diagnosed as alcohol-induced pancreatic steatosis.ResuIts:The distribution of the different ADH2 and ALDH2 genotypes among the 163 alocholics closely conformed to expected Hardy-Weinberg frequencies(p>0.05).In drinkers,compared with ADH2*2/*2 carriers,ADH2*1/*1 carriers showed a significantly elevated risk of developing pancreatic steatosis(50 years,OR=5.34).No association was found between ALDH2 genotypes and risk of pancreatic steatosis.Conclusion.In drinkers,ADH2*1/*1 carriers had a significantly higher risk to developalcohol-induced pancreatic steatosis.ADH2*l/*lgenotype may be related to alcohol-induced pancreatic steatosis.

关键词： alcohol pancreas steatosis double-echo chemical shift magnetic resonance imaging alcohol dehydrogenase aldehyde dehydrogenase

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Rapid simultaneous genotyping of polymorphisms in ADHIB and ALDH2 using high resolution melting assay

Rapid simultaneous genotyping of polymorphisms in ADHIB and ...

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第十二次全国医学遗传学学术会议

作者： Xiao-Wen Yuan Wan-Jun Zhou Xuan Shang Liang Li Yan-Hui Liu Xiang-Min Xu Department of Medical Genetics School of Basic Medical SciencesSouthern Medical University

Background:alcohol dehydrogenase-1B(ADH1B) and aldehyde dehydrogenase-2 (ALDH2) are important enzymes in human alcohol metabolism.The genetic polymorphisms of ADH1B 143G>A(ADH1B*1/ADH1B*2) and ALDH2 15100>A(ALDH2*1/ALDH2*2) are responsible for serious alcohol related disorders.A rapid,accurate,sensitive and high-throughput assay for genotyping the two genes was developed,and the assay could be useful in the epidemiological studies and risk evaluation of alcohol-related diseases.Methods:In this study,we designed two amplicons with different melting temperature by adding GC-or AT-rich 5’ tails,and the two amplicons include ADH1B 143G>A and ALDH2 1510G>A respectively.A total of 200 genomic DNA samples from Chinese population were enrolled to evaluate the specificity of the assay.40 samples randomly selected from each of the genotype groups tested by HRM assay were validated by DNA sequencing.The assay reproducibility and detection limit was evaluated by replicate experiments and two-fold serial dilutions of DNA concentration,respectively.Results: All samples were unambiguously distinguished by HRM assay.There was 100%concordance between the results of the HRM assay and DNA sequencing.Meanwhile,the assay has highly reproducibility,and the coefficients of variation were in the range 0.022%to 0.062%.In addition, only 6.25ng of genomic DNA can be detected as well.Conclusion:The method we present is proved to be high-throughput and reliable,which is suitable for rapid simultaneous genotyping of all nine genotype groups of ADH1B and ALDH2 gene in a single closed tube with $0.5 in 2 hours.

关键词： alcohol dehydrogenase aldehyde dehydrogenase genotyping high resolution melting assay

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Theoretical investigation on the enantioselectivity of ketone reduction by Zn-dependent medium-chain dehydrogenase

Theoretical investigation on the enantioselectivity of keton...

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2019中国化学会第十五届全国计算（机）化学学术会议

作者： Shenggan Luo Yi-Lei Zhao State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University

alcohol dehydrogenase（ADH） catalyzes the reversible conversion of alcohol and corresponding ketone or aldehyde, with the concomitant redox of NAD（P）+/NAD（P）H cofactor. The medium-chain dehydrogenase（MDR） family is a class of valuable oxidoreductases that catalyze the formation of chiral alcohols from the correspondingly prochiral ketones. Here we studied an alcohol dehydrogenase from Candida parapsilosis（Cp RCR）, by which a series of aliphatic ketones are reduced into alcohols with the different enantiomeric excess. In particular, the narrow scope of substrates（21 a-24 a） and a few mutants（wild type, W116 A, F285 A, and W286 A） were analyzed by molecular dynamics simulation and QM/MM calculations. Both the negative correlation of kcat-substrates and the unexpected enantioselectivity related to Prelog’s rule were rationalized with the pre-reaction states and reaction barriers. Our calculations show that the PRS model gave consistent results with the experimental observation.

关键词： QM/MM simulation Pre-reaction state medium-chain dehydrogenase alcohol dehydrogenase

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