Alcohol dehydrogenase 5 of Helicoverpa armigera interacts with the CYP6B6 promoter in response to 2-tridecanone

作     者：Jie Zhao Qian Wei Xin-Rong Gu Su-Wei Ren Xiao-Ning Liu Jie Zhao;Qian Wei;Xin-Rong Gu;Su-Wei Ren;Xiao-Ning Liu

作者机构：Xinjiang Key Laboratory of Biological Resources and Genetic EngineeringCollege of Life Sciences and TechnologyXinjiang UniversityUrumqiXinjiangChina Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources UtilizationCollege of AgricultureShihezi UniversityShiheziXinjiangChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2020年第27卷第5期

页      面：1053-1066页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：This work was supported by the National Natural Science Foundation of China(31471781) Natural Science Foun-dation of Xinjiang Region in China(2016D01C042) Tianshan Cedar Project in 2017(2017xs20) 

主　　题：alcohol dehydrogenase DNA-protein interaction Helicoverpa armigera regulatory factor 2-tridecanone 

摘      要：Alcohol dehydrogenase 5(ADH5)is a member of medium-chain dehydro-genase/reductase family and takes part in cellular formaldehyde and S-nitrosoglutathione metabolic network.2-tridecanone(2-TD)is a toxic compound in many Solanaceae crops to defend against a variety of herbivory *** the broader context of insect development and pest control strategies,this study investigates how a new ADHS from Helicoverpa armigera(HaADH5)regulates the expression of CYP6B6,a gene involved in molting and metamorphosis,in response to 2-TD *** of the HaADH5 complementary DNA sequence revealed that its 1002 bp open reading frame encodes 334 amino acids with a predicted molecular weight of 36.5 ***5 protein was purified in the Es-cherichia coli Transetta(pET32a-HaADH5)strain using a prokaryotic expression *** ability of HaADH5 protein to interact with the 2-TD responsive region within the promoter of CYP6B6 was confirmed by an in vitro electrophoretic mobility shift assay and transcription activity validation in ***,the expression levels of both HaADH5 and CYP6B6 were found to be significantly decreased in the midgut of 6th instar larvae after 48 h of treatment with 10 mg/g 2-TD artificial *** results indicate that upon 2-TD treatment of cotton bollworm,HaADHS regulates the expression of CYP6B6 by interacting with its *** HaADH5 regulation of CYP6B6 expression may con-tribute to the larval xenobiotic detoxification,molting and metamorphosis,HaADH5 is *** target for controlling the growth and development of cotton bollworm.