检索结果-南通市图书馆

Metformin promotes angiogenesis and functional recovery in aged mice after spinal cord injury by adenosine monophosphate-activated protein kinase/endothelial nitric oxide synthase pathway

引用

Neural Regeneration Research 2023年第7期18卷 1553-1562页

作者： Jin-Yun Zhao Xiao-Long Sheng Cheng-Jun Li Tian Qin Run-Dong He Guo-Yu Dai Yong Cao Hong-Bin Lu Chun-Yue Duan Jian-Zhong Hu Department of Spine Surgery and Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan ProvinceChangshaHunan ProvinceChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Hunan Engineering Research Center of Sports and Health ChangshaHunan ProvinceChina Department of Sports Medicine Research Centre of Sports MedicineXiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

Treatment with metformin can lead to the recovery of pleiotropic biological activities after spinal cord ***,its effect on spinal cord injury in aged mice remains *** the essential role of angiogenesis during the regeneration process,we hypothesized that metformin activates the adenosine monophosphate-activated protein kinase/endothelial nitric oxide synthase pathway in endothelial cells,thereby promoting microvascular regeneration in aged mice after spinal cord *** this study,we established young and aged mouse models of contusive spinal cord injury using a modified Allen *** found that aging hindered the recovery of neurological function and the formation of blood vessels in the spinal *** with metformin promoted spinal cord microvascular endothelial cell migration and blood vessel formation in ***,intraperitoneal injection of metformin in an in vivo model promoted endothelial cell proliferation and increased the density of new blood vessels in the spinal cord,thereby improving neurological *** role of metformin was reversed by compound C,an adenosine monophosphate-activated protein kinase inhibitor,both in vivo and in vitro,suggesting that the adenosine monophosphate-activated protein kinase/endothelial nitric oxide synthase pathway likely regulates metformin-mediated angiogenesis after spinal cord *** findings suggest that metformin promotes vascular regeneration in the injured spinal cord by activating the adenosine monophosphate-activated protein kinase/endothelial nitric oxide synthase pathway,thereby improving the neurological function of aged mice after spinal cord injury.

关键词： adenosine monophosphate-activated protein kinase/endothelial nitric oxide synthase pathway angiogenesis aged mice compound C metformin spinal cord injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CpG ODN Enhances Immunization Effects of Hepatitis B Vaccine in aged mice

引用

Cellular & Molecular Immunology 2004年第2期1卷 148-152页

作者： WeibingQin JianweiJiang QiaoerChen NingYang YifengWang XiangcaiWei RuqiangOu FamilyPlanningResearchInstituteofGuangdong Guangzhou510600China DepartmentofBiochemistry MedicalSchoolofJinanUniversityGuangzhou510632China

Oligodeoxynucleotides(ODN)containing unmethylated CpG dinucleotides in contexts of unique sequence (CpG motifs)is active as adjuvant in induction of cellular and humoral immune responses in young *** date,there are only limited reports about effect of CpG ODN on immune responses against hepatitis B(HB) infection in aged *** studies demonstrated there were significant increases in secreting of total anti-HB IgG,IgG1 and IgG2a,as well as of IL-12 and IFN-γ,when CpG ODNs were injected together with hepatitis B antigen in aged ***,CpG ODN could stimulate proliferation of spleen lymphocytes in a dose-dependent *** together,the results we obtained indicate that the adding of CpG ODN into the vaccine antigen might be useful in development of more effective vaccination for inducing anti-HB virus responses in the *** & Molecular Immunology.2004;1(2):148-152.

关键词： CpG oligodeoxynucleotide aged mice adjuvant Vaccination

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The effect of adjuvants on vaccine-induced antibody response against influenza in aged mice

引用

Frontiers in Biology 2014年第2期9卷 89-94页

作者： Mark A. CONCANNON Jiu JIANG Department of Biology Drexel University Philadelphia PA 19104 USA

While influenza remains a major threat to public health, researchers continue to search for a universal solution to improving the efficacy of the influenza vaccine. Even though influenza affects people of all different ages, it can be extremely hazardous to people of 65 years of age or older since that is the population that makes up the high majority of the death toll caused by influenza-related diseases. Elderly individuals suffer the effects of immunosenescence as they age, which is the diminishing of the overall immune response. Immunosenescence occurs by specifically affecting the adaptive immune response which controls the establishment of immunity after vaccination or infection. There are many studies under way that are trying to find a resolution to the problem of the influenza vaccine not providing enough protection in the elderly population. One of the possible strategies is to seek the use of an optimal adjuvant, an immunological agent that can enhance immune responses, with the current vaccine formulation. Here, we used the murine model to review the effects of adjuvants on the antibody response to influenza vaccines in aged mice. Since adjuvants can enhance the production of important inflammatory cytokines and activation of dendritic cells, the stimulation of these cells are boosted to increase the effectiveness of the influenza vaccine in aged mice which would hopefully translate to the elderly.

关键词： adjuvant influenza vaccine aged mice

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The effect of docosahexaenoic acid on visual evoked potentials in aged mice

The effect of docosahexaenoic acid on visual evoked potentia...

引用

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Aysel Agar ozlem ozsoy Piraye yargycoglu Akkiraz Mutay aslan Akdeniz University Faculty of MedicineDepartment of PhysiologyAntalyaTurkey Akdeniz University Faculty of MedicineDepartment of BiophysicsAntalyaTurkey Akdeniz University Faculty of MedicineDepartment of BiochemistryAntalyaTurkey

This study aimed to investigate the effect of docosahexaenoic acid(DHA) on visual evoked potentials(VEPs) in aged *** months old C57BL/6 mice were divided as control and DHA-treated(DHA) *** was given daily by gavage for 4 *** were recorded with sub-dermal needle electrodes placed on the head. Retina antioxidant enzyme activities were determined by spectrophotometric *** concentration of

关键词： Docosahexaenoic acid Visual evoked potentials Antioxidant enzymes TBRS aged mice

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Tibolone modulates neuronal plasticity through regulating Tau, GSK3β/Akt/PI3K pathway and CDK5 p35/p25 complexes in the hippocampus of aged male mice

引用

Neural Regeneration Research 2017年第4期12卷 588-595页

作者： Teresa Neri-Gomez Judith Espinosa-Raya Sofia Diaz Cintra Julia Segura-Uribe Sandra Orozco-Suarez Juan Manuel Gallardo Christian Guerra-Araiza Unidad de Investigación Médica en Farmacología Hospital de Especialidades Centro Médico Nacional Siglo XXI Instituto Mexicano del Seguro Social Ciudad de México México Laboratorio de Farmacología Conductual Escuela Superior de Medicina Instituto Politécnico Nacional Plan de San Luis y Díaz Mirón Col. Sto. Tomás Ciudad de México México Departamento de Neurobiología del Desarrollo y Neurofisiología Instituto de Neurobiología Universidad Nacional Autónoma de México Campus Juriquilla Querétaro Querétaro México Enfermedades Neurológicas (Neurological Diseases) Hospital de Especialidades Centro Médico Nacional Siglo XXI Instituto Mexicano del Seguro Social Ciudad de México México Enfermedades Nefrológicas (Kidney Diseases) Hospital de Especialidades Centro Medico Nacional Siglo XXI Instituto Mexicano del Seguro Social Ciudad de Mexico Mexico

Aging is a key risk factor for cognitive decline and age-related neurodegenerative disorders. Also, an age-related decrease in sex steroid hormones may have a negative impact on the formation of neurofibrillary tangles （NFTs）; these hormones can regulate Tau phosphorylation and the principal kinase GSK3β involved in this process. Hormone replacement therapy decreases NFTs, but it increases the risk of some types of cancer. However, other synthetic hormones such as tibolone （TIB） have been used for hormone replacement therapy. The aim of this work was to evaluate the long-term effects of TIB （0.01 mg/kg and 1mg/kg, intragastrically for 12 weeks） on the content of total and hyperphosphorylated Tau （PHF-1） proteins and the regulation of GSK3β/Akt/PI3K pathway and CDK5/p35/p25 complexes in the hippocampus of aged male mice. We observed that the content of PHF-1 decreased with TIB administration. In contrast, no changes were observed in the active form of GSK3β or PI3K. TIB decreased the expression of the total and phosphorylated form of Akt while increased that of p110 and p85. The content of CDK5 was differentially modified with TIB： it was increased at low doses and decreased at high doses. When we analyzed the content of CDK5 activators, an increase was found on p35; however, the content of p25 decreased with administration of low dose of TIB. Our results suggest a possible mechanism of action of TIB in the hippocampus of aged male mice. Through the regulation of Tau and GSK3β/Akt/PI3K pathway, and CDK5/p35/p25 complexes, TIB may modulate neuronal plasticity and regulate learning and memory processes.

关键词： nerve regeneration Tibolone hippocampus aged mice sex steroids Akt GSK3β PI3K neuralplasticity Tau neurofibrillary tangles neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Application of a real-ambient fine particulate matter exposure system on different animal models

引用

Journal of Environmental Sciences 2021年第7期33卷 64-70页

作者： Yuanyuan Song Lifang Zhao Zenghua Qi Yanhao Zhang Guodong Cao Ruijin Li Lin Zhu Zhu Yang Chuan Dong Zongwei Cai State Key Laboratory of Environmental and Biological Analysis Department of ChemistryHong Kong Baptist UniversityHong Kong SARChina Institute of Environmental Science Shanxi UniversityTaiyuan 030006China School of Environmental Science and Engineering Guangdong University of TechnologyGuangzhou 510006China

Simulation of fine particulate matter(PM_(2.5))exposure is essential for evaluating adverse health *** this work,an ambient exposure system that mimicked real atmospheric conditions was installed in Taiyuan,China to study impacts of chronic PM_(2.5) exposure on adult and aged mice as well as Sirtuin3 knockout(Sirt3 KO)mice and wild-type(WT)*** real-ambient exposure system eliminated the possible artificial effects caused from exposure experiments and maintained the physiochemical characteristics of PM_(2.5).The case studies indicated that aged mice exhibited apparent heart dysfunction involving in-creased heart rate and decreased blood pressure after 17-week of real-ambient PM_(2.5) ***,15-week of real-ambient PM_(2.5) exposure decreased the heart rate and amounts of associated catecholamines to induce heart failure in Sirt3 KO ***,the increased pro-inflammatory cytokines and decreased platelet related indices suggested that inflammation *** changes of biomarkers detected by targeted metabolomics confirmed metabolic disorder in WT and Sirt3 KO mice after exposed to real-ambient PM_(2.5).These results indicated that the real-ambient PM_(2.5) exposure system could evaluate the risks of certain diseases associated with air pollution and have great potential for support-ing the investigations of PM_(2.5) effects on other types of rodent models.

关键词： Real-ambient PM_(2.5)exposure system Adverse effects Sirt3 KO aged mice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

灵芝多糖对体外培养的小鼠脾细胞产生IL-2的影响(英文)

引用

北京大学学报(医学版) 1991年第5期23卷 412-416页

作者：马莉林志彬李荣芷何云庆北京医科大学药理学教研室北京医科大学中药研究室

用活化小鼠脾细胞法观察灵芝多糖BNA,BNB,和BNC对体外培养小鼠脾细胞IL-2产生的影响,发现BNA(0.05,0.1,0.5和1μg·ml),BNB(0.05和0.1μg·ml)及BNC(0.05,0.5和1μg·ml)均显著增加正常小鼠脾细胞IL-2的产生;老年小鼠脾细胞IL-2产生明... 详细信息

用活化小鼠脾细胞法观察灵芝多糖BNA,BNB,和BNC对体外培养小鼠脾细胞IL-2产生的影响,发现BNA(0.05,0.1,0.5和1μg·ml),BNB(0.05和0.1μg·ml)及BNC(0.05,0.5和1μg·ml)均显著增加正常小鼠脾细胞IL-2的产生;老年小鼠脾细胞IL-2产生明显低于年轻对照组,BNA(0.001,0.01,0.1和1μg·ml),BNB(0.005,0,5和5μg·ml)及BNC(0.005,0.05和5μg·ml)可恢复小鼠脾细胞产生IL-2的能力;BNA(0.01,0 .和10 μg·ml),BNB(0.05μg·ml)及BNC(0.·ml)可部分拮抗氢化可的松抑制小鼠脾细胞产IL-2的效应;BNA(0.01,0.1,1和10μg·ml),BNB(0.05,0.5和 5μg·ml)及BNC(5 μg·ml)亦分对抗环孢素A对小鼠脾细胞IL-2产生的抑制效应。

关键词： Ganoderma polysaccharides Interleukin 2 Hydrocortisone Cyclosporin A aged mice

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：