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Effects of kallikrein gene transfer on penumbral microvascular proliferation and on regional cerebral blood flow following cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2008年第10期3卷 1045-1050页

作者： Ruiyan Lu Qingyu Shen Lianhong Yang Mei Li Yidong Wang Ying Peng Department of Neurology Second Affiliated Hospital of Sun Yat-sen University Guangzhou 510120 Guangdong Province China

BACKGROUND： Recent findings have demonstrated that the kallikrein-kinin system （KKS） participates in the pathological process of cerebral ischemia/reperfusion injury. Kallikrein gene transfer exhibits neural protective effects following cerebral infarction. OBJECTIVE： To observe the effects of kallikrein gene transfer on vascular proliferation in the peripheral infarct focus and on regional cerebral blood flow （rCBF） following cerebral ischemia/reperfusion injury. DESIGN, TIME AND SETTING： The completely randomized, controlled experiment was performed at the Lin Baixin Laboratory Center, the Second Affiliated Hospital of Sun Yat-sun University between September 2007 and April 2008. MATERIALS： pUCI9-HTK plasmid was constructed and maintained in the Laboratory for Neurology, the Second Affiliated Hospital of Sun Yat-sen University, China. Mouse anti-human kallikrein 1 monoclonal antibody was purchased from R＆D Systems, USA. METHODS Ninety healthy, male, Sprague Dawley rats were used. Middle cerebral artery occlusion （MCAO） was established in all rats to induce cerebral ischemia/reperfusion injury. Following MCAO establishment, all rats were randomly divided into three groups （n = 30）： blank control, saline, and pAdCMV-HTK. The saline and pAdCMV-HTK groups were stereotactically micro-injected with 5μL of physiological saline or with pAdCMV-HTK [multiplicity of infection （MOI） = 20], respectively, into the ischemic penumbra. In the blank control group, only sham injection was performed. MAIN OUTCOME MEASURES： At 12, 24, and 72 hours after treatment, cerebral infarction volume was measured by 2, 3, 5-triphenyltetrazolium chloride （TTC） staining. Exogenous HTK expression, as well as regional vascular endothelial growth factor （VEGF） expression, was detected by immunohistochemistry. rCBF was examined by 14C-iodoantipyrine micro tracing. In addition, neurological severity score （NSS） was performed. Higher scores indicated more severe neurological deficits. RESULTS： NSS results demonstrated that compared with the saline a

关键词： cerebral ischemia ischemia/reperfusion injury adenovirus gene transfer kallikrein vascular endothelial growth factor perfusion, regional

Expression of B7-1 and B7-2 mediated by adenovirus enhanced T cell anti-hepatoma immunity

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中国实验血液学杂志 1997年第3期5卷 311-311页

作者： Hongtian Huang, Xiaoning Wang, Qijun Qian, Feng Shen, Tianpei Xian, Lehua Shi, Han Chen, Mengchao Wu, Yajun GuoTumor Immunology and Gene Therapy Center. Eastern Institute of Hepatobi/iary Surgery, Shanghai 200433

The ultimate aim of cancer immunotherapy is theinduction of tumor-specific T-lymphocyte responseseffective in eradicating disseminated tumors. There aregenerally tumor infiltrating T lymphocytes in situ oftumor, but most of which are inactive, one of the majorreasons of such Inactivation is that T lymphocytes areexposured to an antigen-specific TCR signal in theabsence of antigen-nonspecific or co-stimulatory signal.The co-stimulatory factors, B7-l and B7-2 on the surfaceof antigen presenting cells provide a key signal for thegeneration of T cell immunity. Sincc hepatoma

关键词： hepatoma immunity adenovirus infiltrating immunotherapy inactive lymphocyte ultimate nonspecific disseminated

adenovirus-mediated RIP140 overexpression specially in heart after myocardial infarction accelerated the progress of heart failure

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Adenovirus-mediated RIP140 overexpression specially in heart...

SARS-CoV-2:Possible role in children with acute hepatitis of unknown origin

2012年中美临床和转化医学国际论坛心脑血管病分会论坛...

作者： Yanfang Chen Peiqing Liu~* Department of Pharmacology and Toxicology School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006

＜正＞Objectives:RIP140 is an important corepressor that regulates metabolism homeostasis in many metabolic tissues.However,the expression and precise function of RIP 140 under conditions of cardiac stress were incomp... 详细信息

关键词： Myocardial infarction adenovirus RIP140 Energy metabolism

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hLife

hLife 2023年第2期1卷 83-92页

作者： Renqiang Yu Ailing Chen Yongjun Zhang Department of Neonatology Wuxi Maternity and Child Health Care HospitalAffiliated Women’s Hospital of Jiangnan UniversityJiangsuChina Research Institute for Reproductive Health and Genetic Diseases Wuxi Maternity and Child Health Care HospitalAffiliated Women’s Hospital of Jiangnan UniversityJiangsuChina Department of Pediatrics Xinhua HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina

The UK raised an alert with the World Health Organization(WHO)in April 2022 concerning a significant surge in the num-ber of children diagnosed with acute hepatitis of unknown origin(AHUO).Despite an ongoing investigation attributing its etiology to adenovirus infection,the absence of adenovirus inclusions in liver biopsies and the remarkably low viral load raised doubts.Concurrently,reports of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infections in children coincided with the surge in acute hepatitis cases,prompting the need for a thorough examination of the poten-tial connection between current or past SARS-CoV-2 infections and pediatric hepatitis epidemics.Epidemiological data suggests that acute hepatitis is a notable manifestation of the multiple-system inflammatory syndrome in children following the global SARS-CoV-2 pandemic.However,the precise role of the SARS-CoV-2 virus in liver injury etiology remains uncertain.To shed light on the potential association between SARS-CoV-2 and AHUO in children,we conduct-ed a systematic review of epidemiological links,potential pathogenesis,and recent research findings,aiming to raise awareness and vigilance among clinicians.Convincing and definitive evidence will be essential in the future to confirm the direct impact of SARS-CoV-2 infection on children with AHUO.

关键词： acute hepatitis of unknown origin children severe acute respiratory syndrome coronavirus 2 multiple-system inflammatory syndrome adenovirus

Construction of Ad-EGFP-BDNF vector and its expression in neural stem cells

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Neural Regeneration Research 2010年第13期5卷 987-992页

作者： Shaohua Li Zhenhua Li Huilong Wen Xin Gu Yinjiang Cui Yang Wang Lixia Lu Yanna Li Manli Na Department of Orthopedics Tenth People's Hospital of Tongji University Shanghai 200072 China Department of Anatomy and Histoembryology Fudan University Medical School Shanghai 200040 China Laboratory of Biochemistry and Molecular Biology Department of Regenerative Medicine Tongji University School of Medicine Shanghai 200092 China Department of Pathophysiology Tongji University School of Medicine Shanghai 200092 China New Gene Biology Company of Shanghai Shanghai 200040 China

BACKGROUND： Brain-derived neurotrophic factor （BDNF） provides nourishment to injured neurons. Neural stem cells can differentiate into neurons to repair neuronal injury in vivo. It has been hypothesized that continuous secretion of BDNF from neural stem cells could benefit brain injury repair. OBJECTIVE： To transfect BDNF and enhanced green fluorescent protein （EGFP） into neural stem cells with adenovirus vector and to observe expression of BDNF and EGFP in transfected neural stem cells. DESIGN, TIME AND SETTING： Observational, cellular, molecular study was performed at the Biochemistry Laboratory, Tongji University School of Medicine, China from July 2004 to September 2006. MATERIALS： Neural stem cells were provided by the Anatomy and Histoembryology Laboratory of Fudan University Medical School, China. METHODS： BDNF cDNA was extracted by reverse transcription polymerase chain reaction from the rat hippocampus. Following gene cloning and packaging by HEK293.BDNF, the EGFP gene was transfected into cultured neural stem cells with the Ad-EGFP-BDNF vector. BDNF-expressing neural stem cell clones were selected by G418 selection. MAIN OUTCOME MEASURES： EGFP expression and cell morphology were observed by fluorescent microscopy; neural stem cell expressing BDNF mRNA was examined by reverse transcription polymerase chain reaction; BDNF expression was detected by enzyme-linked immunosorbent assay from supematant of infected neural stem cells. RESULTS： High transfection efficiency was obtained using 5×10^8 virus titers to transfect neural stem cells. G418-resistant neural stem cell clones integrated BDNF mRNA fragments. Enzyme-linked immunosorbent assay results showed that BDNF expression in the supernatant increased with increasing culture time and peaked at 72 hours. CONCLUSION： adenovirus-mediated BDNF and EGFP genes were successfully transfected into neural stem cells and were expressed in neural stem cells for a long period of time.

关键词： neural stem cells brain-derived neurotrophic factor enhanced green fluorescent protein adenovirus immunohistochemistry gene transfection neural regeneration

Prevalence of adenovirus and rotavirus infection in immunocompromised patients with acute gastroenteritis in Portugal

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World Journal of Virology 2015年第4期4卷 372-376页

作者： Joana Ribeiro Delfim Ferreira Célia Arrabalde Sandra Almeida Inês Baldaque Hugo Sousa Virology Service Portuguese Institute of Oncology of Porto Molecular Oncology and Viral Pathology Group Portuguese Institute of Oncology of Porto Research Department Portuguese League Against Cancer (Liga Portuguesa Contra o Cancro-Núcleo Regional do Norte)

AIM: To characterize the prevalence of rotavirus(RV) and adenovirus(Ad V) infections in immunocompromised patients with acute gastroenteritis. METHODS: The presence of RV and Ad V(serotypes 40 and 41) was evaluated in 509 stool samples obtained between January 2009 and December 2010 from 200 immunocompromised patients(83 females and 117 males; median age 21 years old, range 0-72. The diagnosis of infection was performed as a routine procedure and the presence of RV and Ad V(serotypes 40 and 41) was determined by immunochromatography using the RIDA® Quick Rota-Adeno-Kombi kit(r-Biopharm, Darmstadt, Germany). The data analysis and description of seasonal frequencies were performed using computer software IBM® SPSS®(Statistical Package for Social Sciences) Statistics version 20.0 for Mac. The frequencies of infection were compared into different age and gender groups by χ2 test.RESULTS: The study revealed 12.4% Ad V positive samples and 0.8% RV positive samples, which correspond to a prevalence of 6.5% and 1.5%, respectively. Ad V was more frequent between October 2009 and April 2010, while RV was identified in April 2010 and July 2010. The stool analysis revealed that from the 509 samples, 63(12.4%) were positive for Ad V and 4(0.8%) positive for RV, which by resuming the informationof each patient, lead to an overall prevalence of Ad V and RV of 6.5%(13/200 patients) and 1.5%(3/200 patients), respectively. The stratification of the analysis regarding age groups showed a tendency to an increased prevalence of infection in paediatric patients between 0-10 years old. Considering the seasonal distribution of these infections, our study revealed that Ad V infection was more frequent between October 2009 and April 2010, while RV infection was characterized by two distinct peaks(April 2010 and July 2010). CONCLUSION: The overall prevalence of Ad V and RV infection in immunocompromised patients with acute gastroenteritis was 8% and Ad V was the most prevalent agent.

关键词： Viral gastroenteritis adenovirus Rotavirus Immunocompromised host Stool samples

Adenoviral-mediated Hath1-EGFP gene transfer into guinea pig cochlea through intact round window membrane

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Journal of Otology 2008年第1期3卷 18-23页

作者： CHEN Wei HU Yin-yan YANG Shi-ming GUO Wei SUN Jian-he HAN Dong-yi ZHAI Suo-qiang YANG Wei-yan David ZZHe Neurologic Biological centre Institute of OtolaryngologyDepartment of Otorhinolaryngology-Head&Neck SurgeryChinese PLA General HospitalBeijing 100853China Department of Biomedical Sciences Creighton University2500 California PlazaOmahaNE 68175USA

Objective To study expression of adenoviral-mediated Hath1-EGFP gene in the guinea pig cochlea after transfer through intact round window membrane(RWM), and to assess its effects on hearing. Methods Twenty adult guinea pigs were used, of which: 12 were surgically inoculated with Ad-Hath1-EGFP in the bony groove of round window niche, and 8 with artificial perilymph. Auditory brainstem response(ABR) thresholds were determined in all animals before and 5 days after surgery. On post-surgery day 5 and day 14, animals were sacrificed and whole mounts of cochlea and frozen sections were examined. Results ABR tests showed no significant change of hearing after the surgery. Strong fluorescence staining in the cochleae was seen in Ad-Hath1-EGFP groups. The highest levels of gene expression were seen in the post-surgery day 5 group with little decrease on post-surgery day 14.The contralateral cochlea and those in the control groups were free of fluorescence staining. Conclusion The transgenic Hath1-EGFP can be effectively delivered into the inner ear through intact RWM, in an atraumatic manner.

关键词： Gene transfer round window membrane adenovirus guinea pig Hath1

Zinc-finger protein A20 protects hair cells from damage made by high-power microwave

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Journal of Bio-X Research 2019年第3期2卷 132-139页

作者： Feng Tang Xiaoling Chen Lifeng Jia Hai Li Jingya Li Wei Yuan Department of Otolaryngology The First Hospital Affiliated to the Third Military Medical University(Southwest Hospital)ChongqingChina

Inner ear hair cells are important for maintaining hearing.Irreversible damage to hair cells is an important cause of sensorineural deafness.Electromagnetic radiation,especially high-power microwave,is an important threat to human health in modern society and war.However,it is not clear whether high-power microwave has an effect on cochlea hair cells.This study aimed to assess the effects of high-power microwave on cochlear hair cells in guinea pigs,and investigate the potential protection of these cells against high-power microwave-induced damage by recombinant adenovirus A20.Based on experimental results,a 65 W/cm^(2) irradiation density applied to guinea pigs in this study to establish a high-power microwave inner ear injury model.In addition,pAdEeay-1/A20 was injected via a round window into experimental guinea pig cochlea,whereas artificial perilymph was injected into the control group.Auditory function was assessed by testing the auditory brainstem response threshold,and damage to cochlear hair cells was investigated by cell counting and scanning electron microscopy observations of the basilar membrane.Inner ear injury was observed 6 hours after 65 W/cm^( 2 ) of irradiation and the auditory brainstem response threshold was significantly higher in the irradiation group(P<0.05)compared with other groups.Propidium iodide staining and scanning electron microscopy results indicated that significant morphological changes occurred after radiation,especially to inner hair cells,which exhibited remarkable damage and the presence of several unknown spherical substances.Auditory brainstem response threshold was decreased in the pAdEeay-1/A20 group compared with the artificial perilymph group;moreover,damage to hair cells was milder in the pAdEeay-1/A20 group compared with the control group(P<0.01).Thus,high-power microwave can cause damage to cochlear hair cells,as well as hearing loss with prolonged exposure and/or high dosage.In this regard,65 W/cm^( 2 ) of irradiation for 6 hours is a reliable target dose for obser

关键词： cochlea zinc finger protein A20 high power microwave inner hair cells outer hair cells inner ear adenovirus microwave irradiation

Human adenovirus infection in children with acute respiratory tract disease in Guangzhou, China

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Human adenovirus infection in children with acute respirator...