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The GhACS1 gene encoding an acyl-coa synthetase is essential for normal microsporogenesis in early anther development of cotton

The GhACS1 gene encoding an acyl-CoA synthetase is essential...

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2007中国植物生理学会全国学术会议

作者： Wang Xiu-Lan Li Xue-Bao (College of Life Sciences,HuaZhong Normal University,Wuhan 430079,China)

＜正＞Microsporogenesis,associated with the functional expression of many genes,is a highly programmed and regulated process in flowering *** investigate the roles of the genes during anther development,two anther-specific cDNAs（designated GhACS1 and GhACS2）encoding acyl-coa synthetases were isolated from cotton（Gossypium hirsutum）flower cDNA ***,the corresponding GhACS1 gene

关键词： Cotton acyl-coa synthetase Microsporogenesis Gene expression Fatty acid metabolism

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Discovery of a non-adenylating acyl-coa ligase and restoring its adenylation activity

Discovery of a non-adenylating acyl-CoA ligase and restoring...

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中国化学会第十二届全国天然有机化学学术会议

作者： Nan Wang Jeffrey D.Rudolf Liao-Bin Dong Jerzy Osipiuk Catherine Hatzos-Skintges Michael Endres Chin-Yuan Chang Gyorgy Babnigg ANDrzej Joachimiak George N.Phillips Jr Ben Shen Department of Chemistry The Scripps Research Institute Midwest Center for Structural Genomics and Structural Biology Center Biosciences DivisionArgonne National Laboratory Department of Biosciences Rice University Department of Molecular Medicine The Scripps Research Institute Natural Products Library Initiative at The Scripps Research Institute The Scripps Research Institute

acyl-coenzyme A（coa） ligases catalyze the activation of carboxylic acids via a two-step reaction of adenylation followed by ***,we report the discovery of a non-adenylating acyl-coa ligase PtmA2 and the functional separation of an acyl-coa ligase *** PtmA1 and PtmA2,two acyl-coa ligases from the biosynthetic pathway of platensimycin and platencin,are necessary for the two steps of coa *** inactivation of ptmA1 and ptmA2 resulted in the accumulation of free acid and adenylate intermediates,*** and structural characterization of PtmA2 confirmed its ability to only catalyze *** characterization of PtmA2 revealed it binds both free acid and adenylate substrates and undergoes the established mechanism of domain ***,site-directed mutagenesis restored both the adenylation and complete coa activation *** study challenges the currently accepted paradigm of adenylating enzymes and inspires future investigations on functionally separated acyl-coa ligases and their ramifications in biology.

关键词： acyl-coa synthetase acyl-coa ligase platensimycin platencin

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Lipid modification and meiotic cell cycle regulation

Lipid modification and meiotic cell cycle regulation

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第三届细胞结构与功能的信号基础研讨会

作者： Huwei Wang and Jian Zhang Centre for developmental biology,Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Maternal factors play important roles during early animal *** of the major functions of maternal factors is to regulate meiotic cell cycle during *** report here a maternal gene Xlacs,whose mRNA is tightly localized in the vegetal cortex of Xenopus *** is a putative long chain fatty acyl-coa synthetase,whose major products may include palmitoyl-coa, myristoyl-coa and *** of Xlacs mRNA by antisense oligos blocks the first cell cleavage of fertilized eggs,suggesting that Xlacs is essential for cell cycle *** blockage may be resulted from abnormal acceleration of G2/M transition during Xenopus meiosis,which was observed by the *** acceleration can be rescued by co-injection of in vitro transcribed Xlacs mRNAs, suggesting the enzyme activity of XLACS is required for controlled release G2 oocytes into M *** propose that XLACS may negatively regulate cell cycle progression during the meiosis in Xenopus.

关键词： acyl-coa synthetase meiotic cell cycle Xenopus oocytes maternal

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