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Pollen viability of Polygala paniculata L.(Polygalaceae)using different staining methods

BIOCELL 2012年第3期36卷 143-145页

作者： VIVIANE DAL-SOUTO FRESCURA HAYWOOD DAIL LAUGHINGHOUSE IV THAIS SCOTTI DO CANTO-DOROW SOLANGE BOSIO TEDESCO Laboratory of Cytogenetics and Genotoxicity Department of BiologyCentro de Ciências Naturais e Exatas(CCNE)Universidade Federal de Santa Maria(UFSM)Avenida Roraimanº1000Cep.97105-900CamobiSanta MariaRio Grande do SulBrasil Centre for Protein Engineering Institute of Chemistry B6University of LiègeBelgium

Polygala paniculata *** a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was estimated by three different staining methods:2%acetic orcein,2%acetic carmine,and Alexander’s *** young inflorescences of twenty accessions were collected and fixed in a solution of ethanol:acetic acid(3:1)for 24 hours,then stored in ethanol 70%under *** slides per plant,two for each stain,were prepared by squashing,and 300 pollen grains per slide were *** viability was high(>70%)for most accessions of *** using the Alexander’s stain,which proved the most adequate method to estimate pollen viability.

关键词： pollen grains barba-de-bode acetic orcein acetic carmine Alexander’s stain

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Meiotic behavior and pollinic viability in bean cultivars

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BIOCELL 2014年第1期38卷 33-38页

作者： Gracieli DALLA NORA Solange Bosio TEDESCO Tamara PASTORI Viviane Dal-Souto FRESCURA Nerinéia Dalfollo RIBEIRO Haywood Dail LAUGHINGHOUSE IV Sidinei JoséLOPES Maria Teresa SCHIFINO-WITTMANN Laboratory of Cytogenetics and Plant Genotoxicity Department of BiologyCenter for Natural and Exact SciencesFederal University of Santa Maria(UFSM)Rio Grande do SulBrazil

The aims of this study were to determine the meiotic behavior and to estimate pollen grains via-bility in bean(Phaseolus vulgaris L.)*** buds were collected during different developmental stages of the Mesoamerican bean cultivars IAPAR 44,Guapo Brilhante,BRS Expedito,BRS Valente,Guateian 6662 and Pérola,and the Andean bean cultivar Iraí,grown in a *** meiotic index was determined by anther squashing of material fixed in absolute ethanol-glacial acetic acid(3:1)and stained with acetic *** meiotic abnormalities were observed and the meiotic indices were high for all cultivars,indicating that the mismatch generated during crosses is not related to any meiotic *** of pollen viability was made by comparing acetic orcein staining ***’s reactive:pollen viability was high in all cultivars with either stain,but was significantly higher when using the acetic orcein stain(>99%).Though some cultivar showed a significantly smaller pollen size,the range of variation among cultivars was low(means’range 51-66μm).

关键词： acetic orcein Phaseolus vulgaris pollen Alexander’s reactive

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