Pollen viability of Polygala paniculata L.(Polygalaceae)using different staining methods

作     者：VIVIANE DAL-SOUTO FRESCURA HAYWOOD DAIL LAUGHINGHOUSE IV THAIS SCOTTI DO CANTO-DOROW SOLANGE BOSIO TEDESCO 

作者机构：Laboratory of Cytogenetics and GenotoxicityDepartment of BiologyCentro de Ciências Naturais e Exatas(CCNE)Universidade Federal de Santa Maria(UFSM)Avenida Roraimanº1000Cep.97105-900CamobiSanta MariaRio Grande do SulBrasil Centre for Protein EngineeringInstitute of Chemistry B6University of LiègeBelgium 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2012年第36卷第3期

页      面：143-145页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

主　　题：pollen grains barba-de-bode acetic orcein acetic carmine Alexander’s stain 

摘      要：Polygala paniculata *** a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was estimated by three different staining methods:2%acetic orcein,2%acetic carmine,and Alexander’s *** young inflorescences of twenty accessions were collected and fixed in a solution of ethanol:acetic acid(3:1)for 24 hours,then stored in ethanol 70%under *** slides per plant,two for each stain,were prepared by squashing,and 300 pollen grains per slide were *** viability was high(70%)for most accessions of *** using the Alexander’s stain,which proved the most adequate method to estimate pollen viability.