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American Journal of Plant Sciences 2014年第21期5卷 3328-3339页

作者： Babajide Olusegun Omotobora Patrick Olusanmi Adebola David Mxolisi Modise Sunette Marlize Laurie Abe Shegro Gerrano School of Agriculture and Life Sciences University of South Africa Florida South Africa Agricultural Research Council-Vegetable and Ornamental Plants Institute Pretoria South Africa

Sweetpotato is a major staple food in the world. It is a good source of carbohydrates, vitamin A, vitamin C and protein. The maximum production potential of the crop is being hampered by severe drought which ravages most parts of Africa. The main aim of this study therefore was to screen accessions of sweetpotato for drought tolerance in a quick screening method, followed by field screening with a view to identify accessions that can perform well under water stress conditions. Fifty sweetpotato accessions consisting of cultivars and breeding lines collected from the ARC-VOPI gene bank were planted for drought screening in the glass house for six weeks during which water was withheld to induce stress. Observations were made on number of dead plants and days to wilting point. The results were analyzed and 12 best performing accessions were selected for field trials. The field trial was carried out in Lwamondo, Limpopo province, a drought prone area in South Africa, under rain-fed conditions. The best performing accessions were Zapallo, Tacna, Ejumula, 2004-9-2 and Ndou.

关键词： accessions Drought Sweetpotato Tolerance

Genetic Diversity Analysis of Forty Pearl Millet (Pennisetum glaucum(L.) R. Br) accessions from Sudan Using Agronomical Descriptors and DNA Molecular Markers

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Advances in Bioscience and Biotechnology 2018年第7期9卷 322-337页

作者： Hanaa Ibrahim Mohammed Nada Babiker Hamza Agricultural Research Corporation Biotechnology and Biosafety Research Center Khartoum Sudan Commission for Biotechnology & Genetic Engineering National Center for Research Khartoum Sudan

Morphological descriptors and Random Amplification of Polymorphic DNA (RAPD) technique were used to assess the genetic variation among and within forty Pennesitum glaucum accessions from Sudan. accessions were collected from 30 villages representing Darfur, North Kordofan, South Kordofan, and Blue Nile states. 64 amplified fragments were distinguished using ten primers.? 63 bands were polymorphic among the forty accessions with an average of 6.3 polymorphic bands per primer. Low level of genetic similarity was observed (4% - 43%). The PhiPT (analogue of FST fixation index) value for genetic variability obtained over the four regions was 0.169 with high significance (P = 0.01). AMOVA analysis showed higher variance components within regions (80%) than among regions (20%). The two dendrograms obtained by Random Amplification of Polymorphic DNA (RAPD) data;and morphological data based on 26 descriptors did not fit together. PCA (Principal coordinate’s analysis) showed geographic structuring of pearl millet according to its growing regions in Sudan.

关键词： Millet accessions RAPD Morphological Sudan

Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian accessions of White Lupin (Lupinus albusL.) Represent a Unique Genepool

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Open Journal of Genetics 2014年第2期4卷 87-98页

作者： Rosy Raman Raymond B. Cowley Harsh Raman David J. Luckett Graham Centre for Agricultural Innovation (an Alliance between Charles Sturt University and NSW Department of Primary Industries) NSW DPI Agricultural Institute Wagga Wagga Australia

PCR-based genic and microarray-based Diversity Arrays Technology (DArTTM) markers were used to determine genetic diversity in 94 accessions of white lupin (Lupinus albus L.) comprising Australian and foreign cultivars, landraces, and advanced breeding lines from Australian breeding programs. A total of 345 (50 PCR-based and 295 DArT-based) polymorphic fragments were identified, which were used to determine the genetic diversity among accessions. Both cluster analysis of bivariate marker data using UPGMA, and principal coordinate analysis, indicated a high level of genetic diversity in the germplasm. Our results showed that both types of markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a very distinct and separate grouping with both marker systems. Australian cultivars and breeding lines were clustered together and tended to be distinct from European landraces. These findings will allow breeders to select appropriate, diverse parents to broaden the genetic base of white lupin breeding populations.

关键词： Broad-Leaf Lupin Molecular Diversity Germplasm accessions Molecular Markers

Morphological Characterization of Mango （Mangifera indica） accessions Based on Brazilian Adapted Descriptors

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Journal of Agricultural Science and Technology(B) 2013年第11期3卷 798-806页

作者： Ierla Carla Nunes dos Santos Ribeirol Carlos Antonio Femandes Santos Francisco Pinheiro Lima Neto State University of Feira de Santana Feira de Santana 44036-900 Bahia Brazil Embrapa Semidrido Petrolina 56302-970 Pernambuco Brazil

The aim of this study was to characterize 103 mango accessions of the field germplasm collection of Embrapa semi-arid region, located in Juazeiro, Bahia, Brazil and to apply 50 morphological descriptors established by the Brazilian Ministry of Agriculture, livestock and supply to help in the development of new mango cultivars for the Northeastern region of Brazil. Four trees were selected for each accession and eight adult leaves, eight flowers and 16 fruits were collected from each tree. Morphological characteristics ranging from plant size to seed embryo were evaluated. Simple percentages were estimated for all the descriptors. Only the descriptors for leaf symmetry and fruit waxiness did not show variability among the accessions. Eight accessions did not show fruits with fibers, while nine other accessions presented flesh firmness, which is an important characteristic to improve breeding. The soluble solids content was high, above 14 ~Brix for 95% of the accessions with Tommy Atkins showing the lowest value, 12.5 ~Brix. A great diversity was found in the color of the epidermis ranging from green to red. The accessions Amrapali and Salitre presented a dark orange flesh color. The obtained data set, are the most comprehensive so far in Brazil, it allows choosing the best parents to develop new cultivars and will also contribute to the protection of mango cultivars in Brazil.

关键词： Mango germplasm bank accessions morphological descriptors the international union for the protection of newvarieties of plants (UPOV).

Comparison of Proximate Composition and Nutritional Qualities of Fifty-Three Cashew accessions from Burkina Faso

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Food and Nutrition Sciences 2021年第12期12卷 1191-1203页

作者： Judith N. Semporé Laurencia T. Songré-Ouattara Windpouiré Vianney Tarpaga Fabrice Bationo Mamoudou H. Dicko Laboratory of Biochemistry Biotechnology Food Technology and Nutrition (LABIOTAN) UFR-SVT UJKZ Ouagadougou Burkina Faso Food Technology Department Institute of Research for Applied Sciences and Technologies (IRSAT) CNRST Ouagadougou Burkina Faso National Centre for Specialization in Fruits and Vegetables Institute of Environment and Agricultural Research (INERA) CNRST Bobo-Dioulasso Burkina Faso

Cashew cultivation is growing in Burkina Faso, but nut production remains low. Identification of high-performance plant material with known varietal characteristics is essential for breeding. This study consisted of physical-chemical and nutritional characterization of almonds from a core selection of 53 cashew accessions from Burkina Faso. Proximate composition included contents in water, lipids, carbohydrates, proteins, ash, cellulose using standard methods. Major constituents were lipids with an average level of 50.71% ± 4.07%, followed by carbohydrates and proteins with average levels of 21.18% ± 3.81% and 20.62% ± 1.58%, respectively. Average water, ash and cellulose levels were 4.56% ± 0.39%, 2.87% ± 0.27% and 4.61% ± 2.52%, respectively. The analysis of variability within accessions, based on physical and chemical parameters, identified 3 groups that differ in lipids, carbohydrates, proteins, cellulose and energy value. The first group containing 17 accessions of fat-rich cashews, average protein levels and very high energy values is more interesting for tree improvement programs.

关键词： Anacardium occidental L. Cashew Composition accessions Burkina Faso

Genetics of biochemical attributes regulating morpho-physiology of upland cotton under high temperature conditions

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Journal of Cotton Research 2024年第1期7卷 29-44页

作者： MAJEED Sajid CHAUDHARY Muhammad Tanees MUBARIK Muhammad Salman RANA Iqrar Ahmad SHABAN Muhammad TAN Daniel KY JIA Yinhua DU Xiongming HINZE Lori AZHAR Muhammad Tehseen Federal Seed Certification and Registration Department Ministry of National Food Security and ResearchIslamabad 44090Pakistan Horticultural Research Institute National Agricultural Research CentreIslamabad 44000Pakistan Department of Biotechnology University of NarowalNarowal 51600Pakistan Centre of Agricultural Biochemistry and Biotechnology University of AgricultureFaisalabad 38400Pakistan Department of Plant Breeding and Genetics University of Agriculture FaisalabadSub-Campus BurewalaVehariPakistan Faculty of Science School of Life and Environ-mental SciencesPlant Breeding InstituteSydney Institute of AgricultureThe University of SydneySydneyNSW 2006Australia State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agri-cultural ScienceAnyang 455000China Agricultural Research Service USDASouthern Plains Agricultural Research CentreCollege StationTX 77845USA Department of Plant Breeding and Genetics University of AgricultureFaisal-abad 38400Pakistan School of Agriculture Sciences Zhengzhou UniversityZhengzhou 450000China.

Background Cotton is a strategically important fibre crop for global textile *** profoundly impacts several countries’industrial and agricultural *** cotton production is continuously threatened by the unpre-dictable changes in climate,specifically high *** heat-tolerant,high-yielding cotton cultivars with wide adaptability to be grown in the regions with rising temperatures is one of the primary objectives of modern cotton breeding ***,the main objective of the current study is to figure out the effective breed-ing approach to imparting heat tolerance as well as the judicious utilization of commercially significant and stress-tolerant attributes in cotton ***,the two most notable heat-susceptible(FH-115 and NIAB Kiran)and tolerant(IUB-13 and GH-Mubarak)cotton cultivars were spotted to develop filial and backcross populations to accom-plish the preceding study *** heat tolerant cultivars were screened on the basis of various morphological(seed cotton yield per plant,ginning turnout percentage),physiological(pollen viability,cell membrane thermostabil-ity)and biochemical(peroxidase activity,proline content,hydrogen peroxide content)*** The results clearly exhibited that heat stress consequently had a detrimental impact on every studied plant trait,as revealed by the ability of crossing and their backcross populations to tolerate high ***,when considering overall yield,biochemical,and physiological traits,the IUB-13×FH-115 cross went over particularly well at both normal and high temperature ***,overall seed cotton yield per plant exhibited a posi-tive correlation with both pollen viability and antioxidant levels(POD activity and proline content).Conclusions Selection from segregation population and criteria involving pollen viability and antioxidant levels concluded to be an effective strategy for the screening of heat-tolerant cotton g

关键词： accessions Biochemical Breeding Cotton Generation mean analysis Heat stress

Assessment of Seed and Tuber Production Potential in Varieties of Sphenostylis stenocarpa(Africa Yam Bean)

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Agricultural Sciences 2019年第7期10卷 870-881页

作者： Dolapo Olalekan Ibirinde Charity Onyenonachi Aremu Kerimu Balogun Lola Oladokun Department of Crop Production and Protection Federal University Wukari Nigeria Landmark University Omu-Aran Nigeria Ladoke Akintola University of Technology Ogbomoso Nigeria

Twenty accessions of African Yam Bean, grown at the Teaching and Research Farm of Ladoke Akintola University of Technology, Ogbomoso during the cropping seasons of May to November, 2014 and 2015 were assessed for genetic diversity and Genotype × Environment interaction effects on trait performance and their ability to produce tuber. The first six Principal Components jointly explained 70.30% of the total variation among the accessions. Vine length, branching pattern, pod number, pod length, seed number, and seed yield contributed mostly to the observed variations. Seed biometric traits were most variable and contributed 52% of total variation. Variance due to genotype accounted for 54.2%, environment 10.5% and G × E interaction accounted for 30.1% of the interaction sum of squares. accessions, G2, G4, G47, G49 and G50 produced tubers, while accessions G6, G15, G31, G32 and G33 nodulated extensively. Conversely, there was a marginal reduction in seed yield in accessions that either produced tubers or nodules.

关键词： Variations Interactions accessions Diversity Environment African Yam Bean

Morphological Diversity of Corn’s (Zea maysL.) Local Cultivar and Improved Varieties in Central and North of Benin

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American Journal of Plant Sciences 2015年第18期6卷 2867-2877页

作者： Hafiz A. Salami Adolphe Adjanohoun Wilfrid Padonou Abdoul-Madjidou Yacoubou Djima Aly Chabi Yallou Haziz Sina Lamine Baba-Moussa Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Faculté des Sciences et Techniques Université d’Abomey-Calavi Cotonou Bénin Centre de Recherches Agricoles Sud Institut National des Recherches Agricoles du Bénin (INRAB) Attogon Benin Programme Technologie Agricole et Alimentaire (PTAA) Institut National des Recherches Agricoles du Bénin (INRAB) Porto-Novo Benin Centre de Recherches Agricoles Nord Institut National des Recherches Agricoles du Bénin (INRAB) N’dali Benin

The better knowledge of a plant genetic biodiversity is based on a prior study of its agro-morphological characteristics. The objective of this study was to investigate the morphological diversity of maize accessions and its structure on the basis of 14 variables. For the experimentation, 43 and 98 maize accessions were planted in three replicates respectively in central and northern Benin following an incomplete randomized block. The mixed model analysis of two factors variance revealed a significant difference for all accessions considering each agro-morphological characteristic evaluated except the germination day of two areas seed accessions. The numerical classification grouped the accessions into four groups in each zone. The stepwise discriminant analysis showed that early characters related to plant height and ear insertion were the variables that discriminated accessions in both zones. Maturity and recovery ears, sensitivity to streak and the germination days are the variables that discriminate accessions of the two areas. These results provide a database for the creation of improved maize varieties that meet the needs of

关键词： accessions Characterization Agro-Morphological Zea mays L. Benin

Agro-Morphological Variability in Eight Bambara Groundnut (Vigna subterranea (L.) Verdc.) Morphotypes from Zinder (Niger)

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Agricultural Sciences 2023年第1期14卷 1-10页

作者： Oumarou Zango Rabiou Abdou Saley Adamou Salamatou Agbo So Timothée Kouassi Bakasso Yacoubou Département de Biologie Faculté des Sciences et Techniques Université André Salifou de Zinder Zinder Niger Laboratoire de Gestion et Valorisation de la Biodiversité au Sahel Département de Biologie Faculté des Sciences et Techniques Université Abdou Moumouni Niamey Niger

Bambara groundnut (Vigna subterranea (L.) Verdc.) has economic importance for the producers and the traders as cash crop since it generates substantial income for households in Niger. The objective of this study was to assess eight (8) Bambara groundnut morphotype from Université André Salifou de Zinder in Niger Republic by their morphological and agronomic characters. The experiment was conducted in a randomized complete block design with three replications during the rainy season of 2020-2021. Data were collected on the Number of leaves, Plant height (cm), Growth habit (cm), Terminal leaflet length, Terminal leaflet width, Number of nodes/stem, Number of stems/plant, Number of pods/plant, Weight of 100 pods (g), Weight of 100 seeds (g), Length of seeds (mm), Width of seeds (mm), Weight of seeds/Plot (kg). There were significant differences for all characters, except Number of leaves, Terminal leaflet length, Terminal leaflet width, Length of seeds (mm) Weight of 100 pods (g), Weight of 100 seeds (g). The morphotypes UZ-VZ-04, UZ-VZ-03, UZ-VZ-06, UZ-VZ-02 and UZ-VZ-05 have demonstrated good performance for grain weight per plot and can be useful for a breeding program.

关键词： Vigna subterranea accessions Agro-Morphological Traits Weight of 100 Seeds