Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (<i>Lupinus albus</i>L.) Represent a Unique Genepool

作     者：Rosy Raman Raymond B. Cowley Harsh Raman David J. Luckett 

作者机构：Graham Centre for Agricultural Innovation (an Alliance between Charles Sturt University and NSW Department of Primary Industries) NSW DPI Agricultural Institute Wagga Wagga Australia 

出 版 物：《Open Journal of Genetics》 (遗传学期刊（英文）)

年 卷 期：2014年第4卷第2期

页      面：87-98页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Broad-Leaf Lupin Molecular Diversity Germplasm Accessions Molecular Markers 

摘      要：PCR-based genic and microarray-based Diversity Arrays Technology (DArTTM) markers were used to determine genetic diversity in 94 accessions of white lupin (Lupinus albus L.) comprising Australian and foreign cultivars, landraces, and advanced breeding lines from Australian breeding programs. A total of 345 (50 PCR-based and 295 DArT-based) polymorphic fragments were identified, which were used to determine the genetic diversity among accessions. Both cluster analysis of bivariate marker data using UPGMA, and principal coordinate analysis, indicated a high level of genetic diversity in the germplasm. Our results showed that both types of markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a very distinct and separate grouping with both marker systems. Australian cultivars and breeding lines were clustered together and tended to be distinct from European landraces. These findings will allow breeders to select appropriate, diverse parents to broaden the genetic base of white lupin breeding populations.