检索结果-南通市图书馆

International Journal of Ophthalmology(English edition) 2018年第8期11卷 1416-1420页

作者： Young Min Park Jong Soo Lee Ji-Myong Yoo Jong Moon Park Seong-Wook Seo In-Young Chun Seong Jae Kim Department of Ophthalmology School of Medicine Gyeongsang National University Jinju 52727 South Korea Gyeongsang National University Changwon Hospital Samjeongja-ro Seongsan-gu Changwon-si Gyeongsangnamdo 51472 South Korea Department of Ophthalmology School of Medicine Pusan National University and Medical Research Institute Pusan National University Hospital Pusan 49241 South Korea Institute of Health Science Gyeongsang National UniversityJinju 52727 South Korea

This study is to investigate the characteristic features of acanthamoeba keratitis（AK） that differentiating it from herpetic epithelial keratitis（HEK） using anterior segment optical coherence tomography（AS-OCT）. Medical records of three eyes of each AK and herpetic keratitis who had AS-OCT examination were reviewed in this study. Slitlamp biomicroscopy and AS-OCT was performed on the initial visit and on every follow-up visits in all patients. In all three AK cases, reflective bands in the corneal stroma that correspond to the area of radial keratoneuritis were observed. The depth of the reflective bands varied in each case. After AK treatment, slit-lamp biomicroscopy confirmed that radial keratoneuritis had resolved and AS-OCT confirmed that reflective bands in the corneal stroma had also disappeared in all patients. Unlike the AS-OCT results found in AK, highly reflective HEK lesions were observed only in the subepithelial area, not in the stroma. AS-OCT seems to be helpful analyzing the specific depth of the lesion which enables to distinguish AK from HEK.

关键词： acanthamoeba optical coherence tomography herpes herpetic keratitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2019年第10期12卷 479-482页

作者： Ranjit Sah Meenu Chaudhary Shusila Khadka Rafael Toledo Lucrecia Acosta Department of Microbiology Tribhuvan University Institute of MedicineKathmanduNepal Department of Ophthalmology BP Koirala Lions Centre for Ophthalmic StudiesTribhuvan University Institute of MedicineKathmanduNepal área de Parasitología Departamento de FarmaciaTecnología Farmacéutica y ParasitologíaFacultad de FarmaciaUniversidad de ValenciaAv.Vicent Andrés Estellés s/n46100 BurjassotValenciaSpain área de Parasitología Departamento de Agroquímica y Medio AmbienteUniversidad Miguel Hernández de ElcheCampus de Sant Joan d′Alacant s/n03550 Sant JoanAlicanteSpain

Rationale: Microbial keratitis caused by coinfection with more than one species of pathogens is a severe condition with an unfavorable prognosis. Patient concerns: An immunocompetent Nepali woman complained of pain in the left eye, redness, watering and decreased vision for 5 months. Interventions: The patient was discarded and accurately diagnosed with coinfection with Fusarium sp. and acanthamoeba sp. The habit of washing the eyes with tap water from a domestic storage tank was the most likely source of infection since it was found to be contaminated with cysts of acanthamoeba sp. The woman received eye drops of fluconazole and natamycin(5%), cefazoline(50 mg/m L), atropine, and tablets of itraconazole(100 mg), which were later switched to eye drops of clotrimazole(1%), natamycin(5%) and voriconazole(1%), and tablets of itraconazole. A full thickness penetrating keratoplasty was performed followed by treatment with eye drops of voriconazole(1%), natamet(5%), ofloxacin, atropine and carboxymethylcellulose for one ***: After treatment, the condition of the patient significantly improved and was discharged one week after keratoplasty. Lessons: This is the first report of acanthamoeba keratitis in Nepal and the first report of coinfection with Fusarium in this country and highlights the importance of early diagnosis of microbial keratitis both in single microorganism infections and coinfections, even in no contact lens wearers.

关键词： acanthamoeba Fusarium Coinfection Microbial keratitis Nepal

Presence of acanthamoeba *** water purification plants in southern England

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2009年第6期2卷 40-42页

作者： Shanmuganathan V Khan NA School of Biological and Chemical Sciences Birkbeck CollegeUniversity of London School of Veterinary Medicine and Science University of NottinghamSutton BoningtonEnglandUK

Objective:To identify the prevalence of acanthamoeba in drinking water treatment plants during the course of the purification ***:Samples were taken from two drinking water purification plants and monitored for the presence of acanthamoeba in order to estimate the removal capacity of treatment methods employed. Water samples were collected at each step in the purification,during the one year survey,and analysed for the presence of acanthamoeba *** plating on bacterial-seeded ***:The results showed that amoebae were present in surface raw waters in 100%of the samples *** *** isolated from 71%and 57%of the water samples collected from post flat-bottom clarifier 1 and post-sedimentation plant *** the outflow drinking waters,the removal capacity was 100%in both purification plants *** occurrence of acanthamoeba was not associated with ***: These findings confirm that water purification plants employing methods of flocculation,sedimentation,and filtration in combination with activated charcoal filtration,ozonisation and chlorination exhibited sufficient acanthamoeba removal capacity and the presence of amoebae in the tap water may be due to older plumbing,water storage tanks,tap water hygiene,and/or environmental settings.

关键词： acanthamoeba Drinking water Purification plant

Domestic and Peri-Domestic Study Reveals the Presence of Probably <i>acanthamoeba castellanii</i>

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Microbiology 2021年第6期11卷 303-316页

作者： Obed Nanjul Goselle Oluwanifemi Rhoda Abiola Blessing Ene Ogbe Echo Stephen Unazi Sani Peter Ugbedeojo Nerat Lamba Zakat Malchit Nankap Ladong Shedrack Sunday Udoh Oluseemka Charles Ejete Ojochemi Sunday Idoko Yahaya Mbaya Ahmadu Godwin Yandu Ajiji Joseph Terhema Sunday Patience Igeh Henrietta Oluwatoyin Awobode Godwin Nyiutaha Imandeh Bernard Malau Matur Applied Entomology and Parasitology Unit Department of Zoology University of Jos Jos Nigeria Health and Development Support Programme Jos Nigeria Department of Microbiology Plateau State University Bokkos Nigeria Department of Zoology University of Ibadan Ibadan Nigeria Department of Zoology Federal University of Agriculture Makurdi Benue-State Nigeria

acanthamoeba is free living amoeba consisting of many species that are naturally pathogenic and have been isolated from different environmental sources. The purpose of this study was to determine the prevalence and relative abundance of acanthamoeba species in soil and water samples within the University of Jos environment and to create public health awareness on the dangers of this parasitic protozoan. The study was conducted in the eleven (11) Faculties of the University of Jos. Soil and water samples were collected from each of the faculties, cultured and morphologically identified for positive samples. In addition, quantitative data on occurrence were examined to help better understand the potential risk to the university community. The prevalence of acanthamoeba based on the chi-square analysis indicates that there is a significant difference between the number of acanthamoeba species in the soil and water samples collected (P acanthamoeba based on the Man-Whitney test indicates that there was no significant difference in the distribution of acanthamoeba species in the water and soil samples between different faculties (P > 0.05). Morphological identification indicates the presence of probably acanthamoeba castellani. The demonstration of the presence of acanthamoeba species in soil and water sources calls for awareness among the clinical community, as cases of keratitis and granulomatous amoebic encephalitis might have never been recorded due to lack of expertise or unawareness amongst the clinical community. An improved treatment of water supply and strict adherence to water act needs to be strictly encouraged.

关键词： acanthamoeba acanthamoeba castellani Water Soil Jos-Nigeria

In Vitro Activity of Squaramides and Acyclic Polyamine Derivatives against Trophozoites and Cysts of acanthamoeba castellanii

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2018年第8期6卷 1-14页

作者： M.J.Rosales M.Ximenis A.Costa C.Rotger D.Romero F.Olmo E.Delgado M.P.Clares E.Garcia-Espana C.Marin MSanchez Departamento de Parasitologia Facultad de CienciasUniversidad de GranadaGranadaSpain Universidad de las Islas Baleares Palma de MallorcaSpain Universidad de Valencia ValenciaSpain

Pathogenic strains of acanthamoeba cause keratitis (AK), granulomatous amoebic encephalitis (GAE), amoebic pneumonitis (AP), and skin infection in human and animals. The treatment of an acanthamoeba infection is invariably very difficult and not always effective, and compounds that are amebicidic or amebistatic are frequently toxic and/or irritating for humans. Squaramides and polyamine derivatives have been demonstrated to have antitumor and antiprotozoal activity. The aim of this study was to investigate the activity of 5 squaramides and 5 acyclic polyamines against trophozoites and cysts of A. castellanii Neff. Amoebicidal activity against the trophozoites and cytotoxicity against Vero cells were evaluated with a colorimetric assay, using Alamar Blue?, and chlorhexidine digluconate was assayed as the reference drug. The squaramides 3 and 5 and the acyclic polyamine 6 appeared to be the most active against the trophozoites and their cytotoxicity was low, showing selectivity indexes of 28.3, 26, and 25.7, respectively, similar to the control drug, chlorhexidine digluconate (27.6). But only the squaramide 3 showed complete cysticidal activity at the concentrations of 100 and 200 μM, as the chlorhexidine digluconate. Further studies of the mechanism of action and in vivo assays are needed, but squaramide 3 could be used for developing novel therapeutic approaches against acanthamoeba infections.

关键词： acanthamoeba Squaramides Acyclic Polyamines Amoebicidal Cysticidal

A review of acanthamoeba keratitis in the Middle East and Iran

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Acute Disease 2019年第4期8卷 133-141页

作者： Mohammad Hossein Feiz Haddad Azar Shokri Habib Habibpour Seyede Manizhe Heidar Nejadi Infectious and Tropical Diseases Research Center Health Research InstituteAhvaz Jundishapur University of Medical SciencesAhvazIran Department of Parasitology Faculty of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran Vector-borne Disease Research Center North Khorasan University of Medical SciencesBojnurdIran Student Research Committee Ahvaz Jundishapur University of Medical SciencesAhvazIran Department of Parasitology Faculty of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran Shoushtar Faculty of Medical Sciences ShoushtarIran

acanthamoeba keratitis, a vision-threatening disease, is caused by a free-living amoeba of genus acanthamoeba. Amoeba is widely distributed all around the world. Several cases of acanthamoeba keratitis have been reported from the Middle East and Iran as well. The infection is mainly caused by inappropriate use of contact lens;therefore, it is more common among lens wearers. The number of reported cases worldwide is increasing annually due to the increasing number of contact lens wearers for medical or cosmetic reasons. It is known that early diagnosis and treatment can reduce consequent damages, while the delay in these processes will deteriorate the vision. Nowadays contact lens wearing, surgery and use of corticosteroids are known to predispose to acanthamoeba keratitis, and it was also believed that trauma was the main cause. Increasing public knowledge about acanthamoeba infection, inquiring the history of patients, clinical signs and laboratory findings can be helpful to early diagnosis and better treatment. Regardless of the increasing knowledge of diagnosis and treatment, this disease is still a challenge. Considering the long curative time, and insufficient efficacy of available treatments, it seems that the prevention is more important than the treatment. This review aimed to explain acanthamoeba keratitis in the Middle East and Iran from the aspects of epidemiology, diagnosis, and therapeutic treatment.

关键词： acanthamoeba keratitis Diagnosis Therapy Middle East Iran

Atypical Presentation of acanthamoeba Keratitis in a Contact Lens Wearer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Ophthalmology 2020年第4期10卷 261-267页

作者： Nada Al-Yousuf Khalid Al Sindi Shaima Hashim Hasan Alsetri Catherine Dombroski Department of Ophthalmology King Abdullah Medical City Arabian Gulf University Manama Kingdom of Bahrain Departments of Pathology Blood Bank and Laboratory Medicine King Hamad University Hospital Muharraq Kingdom of Bahrain Department of Ophthalmology Salmaniya Medical Complex Manama Kingdom of Bahrain Department of Chemistry and Biochemistry University of California Los Angeles CA USA

acanthamoeba keratitis is a serious infection that can lead to loss of vision. It is highly challenging and often poses a diagnostic dilemma, causing delay in diagnosis and treatment. We report herewith the clinical and histopathology findings of a patient with an atypical presentation of acanthamoeba keratitis in Bahrain. The patient is a 16-year-old Bahraini teenager who was a cosmetic contact lens wearer. She presented with clinical signs and symptoms of microbial keratitis, which was initially misdiagnosed elsewhere as a case of herpetic corneal infection. Her corneal biopsy confirmed the clinical diagnosis as acanthamoeba keratitis. The patient was started on anti amoebic treatment. The infection got eradicated. The cornea healed with a central scar. Eventually, she underwent penetrating keratoplasty. This case report serves to raise awareness of this rare condition. Clinicians should have a high index of suspicion when diagnosing such cases among contact lens wearers. Early diagnosis and treatment are crucial to prevent serious complications.

关键词： acanthamoeba Keratitis Contact Lens Perineuritis Hypopyon

Riboflavin-UVA collagen cross-linking for the treatment of acanthamoeba keratitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Annals of Eye Science 2019年第1期4卷 23-28页

作者： Choul Yong Park Roy S.Chuck Department of Ophthalmology Dongguk UniversityIlsan HospitalGoyangSouth Korea Department of Ophthalmology and Visual Sciences Montefiore Medical CenterAlbert Einstein College of MedicineBronxNYUSA

In this review,recent studies regarding riboflavin-ultraviolet A(UVA)collagen cross-linking for the treatment of acanthamoeba keratitis(AK)were *** written studies about acanthamoeba,keratitis,riboflavin and collagen cross-linking were retrieved from PubMed search engine(***/pubmed).Although there were significant numbers of cases reporting the effectiveness of riboflavin-UVA collagen cross-linking in AK,experimental studies(in vivo and in vitro)failed to verify amoebicidal or cysticidal effect of riboflavin-UVA collagen *** conclusion,the efficacy of riboflavin-UVA collagen cross-linking for the treatment of AK is still *** is necessary to conduct a prospective case-control study for clear guidance for clinicians.

关键词： acanthamoeba keratitis collagen ultraviolet A(UVA) riboflavin cross-linking

Anti-encystment and amoebicidal activity of Lonicera japonica Thunb. and its major constituent Chlorogenic acid in vitro

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2016年第9期9卷 844-850页

作者： Tooba Mahboob Abdul-Majid Azlan Tian-Chye Tan Chandramathi Samudi Shamala Devi Sekaran Veeranoot Nissapatorn Christophe Wiart Department of Parasitology Faculty of MedicineUniversity of Malaya Department of Medical Microbiology Faculty of MedicineUniversity of Malaya School of Pharmacy University of Nottingham Malaysia CampusJalan 56000 Semenyih

Objective:To examine the acanthamoebicidal effects of ethyl acetate,aqueous and butanol fractions of dried flower buds of Lonicera japonica(***) Thunb.(Flos Lonicerae) in ***:acanthamoeba triangularis isolates were obtained from environmental water samples and identified by *** were exposed to ethyl acetate,water and butanol fractions of *** *** concentrations ranging from 0.5 mg/m L to 1.5 mg/m *** extracts were evaluated for growth inhibition at 24,48 and 72 h,*** acid at a concentration of 1 mg/mL was examined for inhibition of ***:Ethyl acetate fraction at a concentration of 1.5 mg/mL evoked a significant reduction of trophozoite viability by 48.9% after 24 h,49.2% after 48 h and 33.7% after 72 h Chlorogenic acid,the major active constituent of *** *** the concentration of 1 mg/mL reduced the cysts/trophozoite ratio by 100% after 24 h,84.0% after 48 h and 72.3% after 72 *** phenolic compound at concentration of 1 mg/mL concurrent with 0.6% hydrogen peroxide inhibited hydrogen peroxide-induced encystment by 92.8% at 72 ***:Results obtained from this study show that ethyl acetate fraction at 1.5 mg/m L is the most potent fraction of *** *** its major constituent chlorogenic acid showed the remarkable inhibition of encystment at a concentration of 1 mg/mL.

关键词： acanthamoeba spp. Amoebicidal Chlorogenic acid Encystment Lonicera japonica Thunb.

Antiacanthamoebic properties of natural and marketed honey in Pakistan

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 967-972页

作者： Farzana Abubakar Yousuf Malik Hassan Mehmood Abdul Malik Ruqaiyyah Siddiqui Naveed Ahmed Khan Department of Biological and Biomedical Sciences Aga Khan University Department of Biological Sciences Faculty of Science and Technology Sunway University

Objective: To determine antiacanthamoebic activity of natural and marketed honey ***: Natural honey samples were collected directly from the bee hive and marketed honey samples were purchased from the local market in Karachi, Pakistan. Both honey samples were tested for their flavonoid content(quercetin equivalent per gram of the extract) and phenolic content(gallic acid equivalent per gram). Furthermore, their antioxidant activity was determined by measuring 2,2-diphenyl-1-picrylhydrazyl. Using amoebistatic and amoebicidal assays, the effects of honey samples were tested against growth and viability of acanthamoeba ***: Natural honey exhibited potent amoebistatic and amoebicidal effects, in a concentration-dependent manner. Honey-treated acanthamoeba castellanii showed loss of acanthopodia, following which amoebae detached, rounded up, reduced in size,decreased in cytoplasmic mass and they were observed floating in the culture ***, honey-treated amoebae did not revive when inoculated in fresh growth medium, however, glycerol-treated amoebae exhibited viable trophozoite and active growth. In contrast, marketed honey samples varied in their efficacy against acanthamoeba castellanii. The proportion of flavonoid, as determined by quercetin measurements and the proportion of phenolic, as determined by gallic acid measurements was higher in natural honey compared with marketed honey. Similarly, the antioxidant activity, as determined by 2,2-diphenyl-1-picrylhydrazyl scavenging activity was higher in natural honey vs. marketed ***: This study shows that natural honey has antiacanthamoebic properties and possesses higher flavonoid, phenolic and antioxidant properties compared with the marketed honey. These findings are of concern to the public, health officials, and to the manufacturers regarding production of honey for medical applications.

关键词： Honey acanthamoeba Amoebicidal Amoebistatic