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zhenxin anshen formula(镇心安神方)ameliorates atopic der-matitis-like skin dysfunction in mice and in vitro via regulation of transient receptor potential vanilloid 1 and transient receptor potential ankyrin 1 in Neural pathways

引用

Journal of Traditional Chinese Medicine 2023年第5期43卷 887-896页

作者： ZHAO Yiding YAN Xiaoning JIANG Shanshan LIU Yong DONG Chun CHI Huiyan MAO Chaoyi Department of Dermatology Shaanxi Provincial Hospital of traditional Chinese MedicineXi’an 710003China Department of Dermatology Xiyuan Hospital China Academy of Chinese Medical SciencesBeijing 100193China Department of Education Management China Academy of Chinese Medical SciencesBeijing 100700China

OBJECTIVE:To investigate the efficacy of zhenxin anshen formula(镇心安神方,ZXAS)on atopic dermatitis(AD)by transient receptor potential vanilloid 1(TRPV1)and transient receptor potential ankyrin 1(TRPA1)signalling pathway in mice and in ***:AD-like lesions were induced by 1-chloro-2,4-dinitrobenzene(DNCB)to the shaved dorsal skin of BALB/c ***/c mice were divided into five groups:normal control,model control,cetirizine,low-,medium-,and high-dose of *** ZXAS in-tervention,the skin lesions and blood samples were collected for hematoxylin and eosin-stained and measuring the concentrations of inflammatory *** E(IgE),interleukin(IL)-4,IL-5,IL-13,and thymic stromal lymphopoietin(TSLP)were de-tected by Enzyme-linked immunosorbent assay(ELISA).The spinal cords were collected for measuring the expression of gastrin-releasing peptide receptor(GRPR),TRPV1,and TRPA1 by using immunohistochemistry,western blotting,and quantitative real-time polymerase chain reaction(qR T-PCR)*** addition,3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)assay,flow cytometry,ELISA,and Western blotting were conducted for analysis of primary dorsal root ganglia(DRG)neurons in ***:ZXAS treatment improved DNCB-induced AD-like lesions through reducing dermatitis score,number of scratching and epidermal thickness,accompanied by the de-creased IgE and Th2 inflammatory *** also supressed the mRNA and protein expression of GRPR,TRPV1,and TRPA1 in the spinal *** medicated sera of ZXAS decreased capsaicin-induced Ca^(2+)influx and downregulated the expression of TRPV1,TRPA1,and phospholipase C in DRG ***:The therapeutic effect of ZXAS on AD may be related to the regulation of TRPV1 and TRPA1 and inhibition of Ca^(2+)signals in neurons.

关键词： dermatitis atopic pruritus neural pathways transient receptor potential vanil-loid 1 transient receptor potential ankyrin 1 zhenxin anshen formula

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