Zhenxin Anshen formula(镇心安神方)ameliorates atopic der-matitis-like skin dysfunction in mice and in vitro via regulation of transient receptor potential vanilloid 1 and transient receptor potential ankyrin 1 in Neural pathways

作     者：ZHAO Yiding YAN Xiaoning JIANG Shanshan LIU Yong DONG Chun CHI Huiyan MAO Chaoyi ZHAO Yiding;YAN Xiaoning;JIANG Shanshan;LIU Yong;DONG Chun;CHI Huiyan;MAO Chaoyi

作者机构：Department of DermatologyShaanxi Provincial Hospital of traditional Chinese MedicineXi’an 710003China Department of DermatologyXiyuan Hospital China Academy of Chinese Medical SciencesBeijing 100193China Department of Education ManagementChina Academy of Chinese Medical SciencesBeijing 100700China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2023年第43卷第5期

页      面：887-896页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China:Study on the Mechanism of Zhenxin Anshen Formula Regulating GRPR/MrgprA3/TRPs Signaling Pathway for Atop-ic Dermatitis based on the Theory of All Kinds of Diseases with Pain Itching and Sores are Exclusively Related to the Heart No.81704087 the Regulation of Zhenxin Anshen Formula on Neuroimmune Function in Atopic Dermatitis from STIM1-ORAI1 Mediated Store Oper-ated Calcium Entry No.82274537 Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences:Based on PAR2/TRPV1 Pathway to Regulate Nerve-Epidermal Pruritus Information Transmission to Explore the Mechanism of Longmu Decoction on“Stress-Type Atopic Dermatitis”Mice No.CI2021A02315 Belt and Road Initiative Cooperation Project of Traditional Chinese Medicine of the Chinese Academy of Traditional Chinese Medicine:Evaluation of the Effect of Chinese Medicine on Allergic Diseases and Material Basis Research No.GH201910 

主　　题：dermatitis atopic pruritus neural pathways transient receptor potential vanil-loid 1 transient receptor potential ankyrin 1 Zhenxin Anshen formula 

摘      要：OBJECTIVE:To investigate the efficacy of Zhenxin Anshen formula(镇心安神方,ZXAS)on atopic dermatitis(AD)by transient receptor potential vanilloid 1(TRPV1)and transient receptor potential ankyrin 1(TRPA1)signalling pathway in mice and in ***:AD-like lesions were induced by 1-chloro-2,4-dinitrobenzene(DNCB)to the shaved dorsal skin of BALB/c ***/c mice were divided into five groups:normal control,model control,cetirizine,low-,medium-,and high-dose of *** ZXAS in-tervention,the skin lesions and blood samples were collected for hematoxylin and eosin-stained and measuring the concentrations of inflammatory *** E(IgE),interleukin(IL)-4,IL-5,IL-13,and thymic stromal lymphopoietin(TSLP)were de-tected by Enzyme-linked immunosorbent assay(ELISA).The spinal cords were collected for measuring the expression of gastrin-releasing peptide receptor(GRPR),TRPV1,and TRPA1 by using immunohistochemistry,western blotting,and quantitative real-time polymerase chain reaction(qR T-PCR)*** addition,3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)assay,flow cytometry,ELISA,and Western blotting were conducted for analysis of primary dorsal root ganglia(DRG)neurons in ***:ZXAS treatment improved DNCB-induced AD-like lesions through reducing dermatitis score,number of scratching and epidermal thickness,accompanied by the de-creased IgE and Th2 inflammatory *** also supressed the mRNA and protein expression of GRPR,TRPV1,and TRPA1 in the spinal *** medicated sera of ZXAS decreased capsaicin-induced Ca^(2+)influx and downregulated the expression of TRPV1,TRPA1,and phospholipase C in DRG ***:The therapeutic effect of ZXAS on AD may be related to the regulation of TRPV1 and TRPA1 and inhibition of Ca^(2+)signals in neurons.