检索结果-南通市图书馆

Screening of hepatocyte proteins binding to complete S protein of hepatitis B virus by yeast-two hybrid system

World Journal of Gastroenterology 2005年第25期11卷 3899-3904页

作者： Gui-QinBai JunCheng Shu-LinZhang Yan-PingHuang LinWang YanLiu Shu-MeiLin FirstHospitalofXi'anJiaotongUniversity Xi'an710004ShaanxiProvinceChina InstituteofInfectiousDiseases DitanHospitalAnwaiStreetBeijing100011China FirstHospitalofXi'anJiaotongUniversity Xi'an710004ShaanxiProvinceChina

AIM: To investigate the biological function of complete S protein and to look for proteins interacting with complete S protein in hepatocytes. METHODS: We constructed bait plasmid expressing complete S protein of HBV by cloning the gene of complete S protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (a type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we underwent sequence analysis by bioinformatics. RESULTS: Nineteen colonies were selected and sequenced. Among them, five colonies were Homo sapiens solute carrier family 25, member 23 (SLC25A23), one was Homo sapiens ***, one was human serum albumin (ALB) gene, one was Homo sapiens metallothionein 2A, two were Homo sapiens betaine-homocysteine methyltransferase, three were Homo sapiens Na+ and H+coupled amino acid transport system N, one was Homo sapiens CD81 antigen (target of anti-proliferative antibody 1) (CD81), three were Homo sapiens diazepam binding inhibitor, two colonies were new genes with unknown function. CONCLUSION: The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with complete S protein of HBV. The complete S protein may bind to different proteins i.e., its multiple functions in vivo.

关键词： Complete S protein yeast-two hybrid system Hepatitis B virus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Screening of hepatocyte proteins binding to NS5ABP37 protein by yeast-two hybrid system

引用

Journal of Pharmaceutical Analysis 2009年第4期21卷 234-237,251页

作者： Lei Zhang1,Qing-yong Ma1,Xian-kui Meng1,Kang Li1,Jun Cheng21.The First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061 2.Institute of Infectious Diseases,Beijing Ditan Hospital,Beijing 100011,China First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China Institute of Infectious Diseases Beijing Ditan Hospital Beijing 100011 China

Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in *** We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus(HCV)by cloning the gene of NS5ABP37 protein into pGBKT7,then the recombinant plasmid DNA was transformed into yeast AH109(α type).The transformed yeast AH109 was mated with yeast Y187(α type)containing liver cDNA library plasmid in 2×YPDA *** yeast was plated on synthetic dropout nutrient medium(SD/-Trp-Leu-His-Ade)containing X-α-gal for selection and *** extracting and sequencing of plasmids from positive(blue)colonies,we made a sequence analysis by *** We screened twenty-five proteins binding to NS5ABP37,including Homo sapiens cyclin I(CCNI)gene,Homo sapiens matrix metallopeptidase 25(MMP25)and Homo sapiens talin *** The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of *** the biological function of NS5ABP37 may be associated with glycometabolism,lipid metabolism and apoptosis.

关键词： NS5ABP37 yeast-two hybrid system hepatitis C virus(HCV)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：