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Investigation on the mechanism of yqhx against cerebral ischemic injury based on network pharmacology and molecular docking

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Journal of Chinese Pharmaceutical Sciences 2022年第2期31卷 117-133页

作者： Yan Shang Xiaoyuan Lin Tiantian Zhang Lihua Xie Guoheng Hu Hunan University of Chinese Medicine Changsha 410208China The First Hospital of Hunan University of Chinese Medicine Changsha 410007China

Cerebral ischemia(CI)is the world’s second-largest lethal disease,with a high recurrence and teratogenic *** Chinese medicine(TCM)yqhx(a pharmaceutical preparation from herbs)has a certain effect in the clinical treatment of CI,while its underlying mechanism remains largely *** explore the potential mechanism,we used network pharmacology and molecular docking in the present *** and CNKI databases were used to explore the active ingredients of yqhx;the Pharmmapper database was used to get the ingredient targets;the OMIM,GeneCards,and DisGeNET databases were used to obtain the disease targets;the Venn diagram was used to obtain the intersection targets,the Cytoscape was used to visualize results and plug-in MCODE to obtain core targets;the Metascape database was used to perform GO and KEGG pathway enrichment analyses on core *** top 20 KEGG pathway enrichment pathways were used to construct the“ingredient-target-pathway”network by Cytoscape;the top 10 ingredients and the top five protein targets were used for molecular docking with AutoDock Vina software,and PyMoL and Ligplus software were used to visualize the results.A total of 83 active ingredients were screened from ***,432 corresponding targets,2005 disease-related targets,and 140 drug-disease intersection targets were *** biological function and KEGG pathway enrichment analyses yielded 507 biological function entries and 141 signaling *** pathway enrichment was mainly involved in cell proliferation,adhesion,migration,and other *** docking results showed that the key ingredients and core targets screened had a strong binding activity,including EGFR,MAP2K1,and *** combination of miltionone I and miltiodiol was relatively *** main biological mechanism of yqhx in the treatment of CI might play a role through the signaling pathway related to the tyrosine kinase receptor,which was also the improvement of the theory of“benef

关键词： Network pharmacology Molecular docking yqhx Cerebral ischemia Mechanism

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Yiqi Huoxue prescription can prevent and treat post-MI myocardial remodeling through promoting the expression of AMPK signal pathway

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Journal of Traditional Chinese Medical Sciences 2017年第3期4卷 235-244页

作者： Jiangong Wu Shuwen Guo Xi Chen Wenchen Liu Mingjing Zhao Lu Zhang Zhaoduo Su Yuqin Zhang Beijing University of Chinese Medicine Beijing 100029China Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine Internal Medicine of Traditional Chinese Medicine Key Laboratory of the Ministry of EducationBeijing 100700China

Objective:To investigate how Yiqi Huoxue (yqhx) prescription regulates mitochondrial biosynthesis and ATP synthesis via AMP-activated protein kinase (AMPK) and to reveal its molecular mechanism in preventing and treating post-MI myocardial ***:The MI animal model of myocardial infarction were established by ligating Sprague Dawley (SD) rats' left anterior descending coronary arteries;the animals were randomly divided into MI group,yqhx prescription group and perindopril group,and a sham operation group was set at the same *** drug intervention was administered on the 2nd day after surgery,the yqhx group was given Astragalus,angelica,ginseng,Ligusticum wallichii and pseudoginseng,provided by the Dongzhimen Hospital of Beijing University of Chinese Medicine,once per day,at a dose of 21 g/kg body weight/day (the clinical equivalent dose based on a previous study),and the changes in relevant indicators were observed at 1 week and 4 *** (ECG) was used to observe the changes in rat cardiac structure and functions;the morphology-based technique was used to observe the changes in myocardial cells and mitochondria;the expression of AMPK signal pathway-related proteins and mRNA was detected using western blotting and real-time fluorescence quantification respectively,while fluorescence enzyme-labeled method was used for detecting ATP ***:Cardiac structure and functions:Compared with the MI group at 1 week,the yqhx prescription group and the perindopril group exhibited increased LVEF and LVFS (Pall .05).At 4 weeks,both LVEF and LVFS were elevated in the yqhx prescription group (P =.008,.009) and the perindopril group (P =.279,.333),where differences in the later group indicated no statistical significance;the yqhx prescription group and the perindopril group were also featured by reduced LVEDs and LVE

关键词： Myocardial infarction yqhx prescription Mitochondria ATP

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