Yiqi Huoxue prescription can prevent and treat post-MI myocardial remodeling through promoting the expression of AMPK signal pathway

作     者：Jiangong Wu Shuwen Guo Xi Chen Wenchen Liu Mingjing Zhao Lu Zhang Zhaoduo Su Yuqin Zhang 

作者机构：Beijing University of Chinese MedicineBeijing 100029China Dongzhimen Hospital Affiliated to Beijing University of Chinese MedicineInternal Medicine of Traditional Chinese Medicine Key Laboratory of the Ministry of EducationBeijing 100700China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2017年第4卷第3期

页      面：235-244页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This study is supported by the National Natural Science Foundation of China(No.81473552) 

主　　题：Myocardial infarction YQHX prescription Mitochondria ATP 

摘      要：Objective:To investigate how Yiqi Huoxue (YQHX) prescription regulates mitochondrial biosynthesis and ATP synthesis via AMP-activated protein kinase (AMPK) and to reveal its molecular mechanism in preventing and treating post-MI myocardial ***:The MI animal model of myocardial infarction were established by ligating Sprague Dawley (SD) rats left anterior descending coronary arteries;the animals were randomly divided into MI group,YQHX prescription group and perindopril group,and a sham operation group was set at the same *** drug intervention was administered on the 2nd day after surgery,the YQHX group was given Astragalus,angelica,ginseng,Ligusticum wallichii and pseudoginseng,provided by the Dongzhimen Hospital of Beijing University of Chinese Medicine,once per day,at a dose of 21 g/kg body weight/day (the clinical equivalent dose based on a previous study),and the changes in relevant indicators were observed at 1 week and 4 *** (ECG) was used to observe the changes in rat cardiac structure and functions;the morphology-based technique was used to observe the changes in myocardial cells and mitochondria;the expression of AMPK signal pathway-related proteins and mRNA was detected using western blotting and real-time fluorescence quantification respectively,while fluorescence enzyme-labeled method was used for detecting ATP ***:Cardiac structure and functions:Compared with the MI group at 1 week,the YQHX prescription group and the perindopril group exhibited increased LVEF and LVFS (Pall .05).At 4 weeks,both LVEF and LVFS were elevated in the YQHX prescription group (P =.008,.009) and the perindopril group (P =.279,.333),where differences in the later group indicated no statistical significance;the YQHX prescription group and the perindopril group were also featured by reduced LVEDs and LVE