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Ectopic porcine spermatogenesis in murine subcutis： tissue grafting versus cell-injection methods

Asian Journal of Andrology 2009年第3期11卷 317-323页

作者： Takeshi Watanabe Hirofumi Hayashi Kaoru Kita Yoshinobu Kubota Takehiko Ogawa Department of Urology Ashigarakami Hospital Kanagawa 258-0003 Japan Department of Urology Yokohama Minami Kyousai Hospital Yokohama 236-0032 Japan Department of Urology Yokohama City University Graduate School of Medicine Yokohama 236-0004 Japan

Fragments of testis tissue from immature animals grow subcutaneous areas of immunodeficient mice. The same results testes of rodents are injected into the subcutis of nude mice. and develop spermatogenesis when grafted onto are obtained when dissociated cells from immature Those cells reconstitute seminiferous tubules and facilitate spermatogenesis. We compared these two methods, tissue grafting and cell-injection methods, in terms of the efficiency of spermatogenesis in the backs of three strains of immunodeficient mice, using neonatal porcine testicular tissues and cells as donor material. Nude, severe combined immunodeficient （SCID） and NOD/Shi- SCID, IL-2Rγc^null （NOG） mice were used as recipients. At 10 months after surgery, the transplants were examined histologically. Both grafting and cell-injection methods resulted in porcine spermatogenesis on the backs of recipient mice; the percentage of spermatids present in the transplants was 67% and 22%, respectively. Using the grafting method, all three strains of mice supported the same extent of spermatogenesis. As for the cell- injection method, although SCID mice were the best host for supporting reconstitution and spermatogenesis, any difference from the other strains was not signifcant. As NOG mice did not show any better results, the severity of immunodeficiency seemed to be irrelevant for supporting xeno-ectopic spermatogenesis. Our results confirmed that tubular reconstitution is applicable to porcine testicular cells. This method as well as the grafting method would be useful for studying spermatogenesis in different kinds of animals.

关键词： pigs seminiferous tubule spermatogenesis xeno-transplantation

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Seeking the“mother of blood”from human pluripotent stem cells:Are we there yet?

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Genes & Diseases 2017年第3期4卷 123-124页

作者： Fei Li The Editorial Office of Genes&Diseases Chongqing Medical UniversityChongqing400046China

Induced pluripotent stem cells(iPSCs)hold great promise for the treatment of human diseases,including the failure of bone marrow.Incremental progress across the past three and half decades has brought us closer to making hematopoietic stem cells from iPSCs clinical solutions.A recent innovative two-step differentiation approach successfully generated transplantable HSCs from iPSC sources.For clinical translation,the long-term safety of these gene-altered HSCs must be determined.

关键词： Hemaotpoietic stem cells Human iPSC Lentiviral transduction Teratoma Transcription factors xeno-transplantation

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