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Effects of Corticosterone, cAMP, cGMP, Ca^(2+), and Protein Kinase C on Apoptosis of Mouse Thymocytes Induced by x-ray irradiation

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Biomedical and Environmental Sciences 2008年第2期21卷 167-172页

作者： SHOU-LIANG GONG LI-HUA DONG GUANG-WEI LIU PING-SHENG GONG WEN-TIAN LU HONG-GUANG ZHAO xIAO-JING JIA YONG ZHAO Key Laboralory of Radiobiology Unit Ministry of Health （School of Public Health Jilin University）Changchun 130021 Jilin China Transplantation Biology Research Division State Key Laboratory of Membrane and Membrane Biotechnology Institute of Zoology Chinese Academy of SciencesBeijing 100080 China Key Laboratory of Molecular Enzymology and Engineering Ministry of Education Jinlin University Changchun 130023 Jilin China

Objective To observe the effects of signal factors of corticosterone （CS）, cAMP, cGMP, Ca^2＋ and protein kinase C （PKC） on lymphocyte apoptosis in mouse thymus induced by x-rays of 4 Gy in vitro. Methods The DNA lyric rate for thymocytes was measured by fluomspectrophotometry. Results The DNA lyric rate for thymocytes 4-8 hours after irradiation with 2-8 Gy was significantly higher than that in the control （P〈0.01）. As compared with the control, the DNA lyric rate for thymocytes treated with 0.01 μnol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.05-0.4 μg/mL ionomycin （Iono, P〈0.05 or P〈0.01） or 0.05-0.4 ng/mL phorbol myristate acetate （PMA, P〈0.05 or P〈0.01）, respectively, was significantly increased, while the rate for thymocytes treated with 50 ng/mL cGMP was not significantly increased. The DNA lyric rate for thymocytes treated with 0.01 μmol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.2 and 0.4 μg/mL Iono （P〈0.05）, and 0.2 and 0.4 ng/mL PMA （P〈0.05） plus 4-Gy irradiation, respectively, was significantly higher than that treated with single 4-Gy irradiation, while the rate for thymocytes treated with 50 ng/mL cGMP plus 4-Gy irradiation was not increased. When both 0.4 I.tg/mL Iono and 0.4 ng/mL PMA acted on the thymocytes, the DNA lyric rate for thymocytes was significantly higher than that in the control （P〈0.01）, the DNA lytic rate for thymocytes treated with both 0.4 μg/mL Iono and 0.4 ng/mL PMA plus 4-Gy irradiation was significantly higher than that treated with single 4-Gy irradiation （P〈0.05）, but was Iono plus 4-Gy irradiation or 0.4 ng/mL PMA plus 4-Gy irradiation. can promote thymocyte apoptosis induced by larger dose x-rays. not significantly higher than that treated with 0.4 μg/mL Conclusion CS, cAMP, Ca^2＋, and PKC signal factors can promote thymocyte apoptosis induced by larger dose x-rays.

关键词： Corticosterone cAMP cGMP Ca^2＋ PKC x-ray irradiation Lymphocyte Apoptosis

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Effect of x-ray irradiation on threshold voltage of AlGaN/GaN HEMTs with p-GaN and MIS Gates

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Nanotechnology and Precision Engineering 2020年第4期3卷 241-243页

作者： Yongle Qi Denggui Wang Jianjun Zhou Kai Zhang Yuechan Kong Suzhen Wu Tangsheng Chen Science and Technology on Monolithic Integrated Circuits and Modules Laboratory Nanjing Electronic Devices InstituteNanjing 210016China China Electronics Technology Group Corporation No.58 Research Institute Wuxi 204035China

Commercially available AlGaN/GaN high-electron-mobility transistors(HEMTs)are beginning to enter the public scene froma range of suppliers.Based on previous studies,commercial GaN-based electronics are expected to be tolerant to different types of irradiation in space.To test this assumption,we compared the characteristic electrical curves obtained at different x-ray irradiation doses for GaN HEMT devices manufactured by Infineon and Transphorm.The p-GaN-based device was found to be more robust with a stable threshold voltage,whereas the threshold voltage of the device with ametal-insulator-semiconductor gatewas found to shift first in the negative and then the positive direction.This dynamic phenomenon is caused by the releasing and trapping effects of radiation-induced charges in the dielectric layer and at the interface of irradiated devices.As such,the p-GaNgate-based GaN HEMT provides a promising solution for use as an electric source in space.

关键词： p-GaN gate GaN HEMTs x-ray irradiation Threshold voltage

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Wettability manipulation nanorod arrays of magnetic transition metal by x-ray irradiation

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Frontiers of Materials Science 2015年第3期9卷 311-316页

作者： Qian xIE Weipeng WANG Zheng xIE Shuang SHUANG Zhengcao LI Zhengjun ZHANG Key Laboratory of Advanced Materials (MOE) School of Materials Science and Engineering Tsinghua University Beijing 100084 China High-Tech Institute of xi'an Xi'an 710025 China

Wettability manipulation of glancing angle deposited Fe/Co/Ni nanorod arrays was realized by x-ray irradiation in ultra-high vacuum chamber. Reversible transition was also purchased by alternating ethanol immersion and x-ray irradiation. Alkyl group adsorption-desorption mechanism and corresponding morphology depen- dence of wettability manipulation were revealed.

关键词： wettability nanorod array transition metal x-ray irradiation

Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells

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International Journal of Ophthalmology(English edition) 2013年第3期6卷 286-294页

作者： Lu-Bin Yan Kai Shi Zhi-Tong Bing Yi-Lan Sun Yang Shen Department of Surgery the Second Hospital of Lanzhou University Department of Ophthalmology the Second Hospital of Lanzhou University Institute of Modern Physics Chinese Academy of Sciences

AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein protein interaction was used to analyze relationships among proteins. RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. CONCLUSION: Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.

关键词： melanoma cell 2D-LC-MS/MS stable isotope labeling with amino acids proteomic analysis x-ray irradiation protein-protein interaction

αB-crystallin malondialdehyde,superoxide dismutase, and lutathione peroxidase changes in x-ray irradiated rat lens

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International Journal of Ophthalmology(English edition) 2011年第4期4卷 365-370页

作者： Guo-xu xu, Guo-Tong xu Department of Ophthalmology the Second Hospital of Soochow University Suzhou 215004 Jiangsu Province China Tongji Eye Institute and Department of Regenerative Medicine Tongji University School of Medicine Shanghai 200092 China

AIM: To evaluate αB-crystallin malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) changes in x-ray irradiated rat lens. METHODS: Eight-week-old Sprague-Dawley male rats received x-ray irradiation to the head with rest of the body protected. The exposure dose ranged from 2 to 25 Grays (Gy). The cataract status were examined by slit lamp and rated with 'four-grade systems' post-irradiation. The lens MDA level, and the activities of SOD and GPx were measured in a short-term experiment post-irradiation, and αB-crystallin protein levels were quantified. RESULTS: The lenses of normal control and the x-ray irradiated groups with the dose up to 10 Gy remained transparent throughout the experiment. The lens first appeared tiny scatters, and even lamellar opacities in the posterior capsule 45 days post-irradiation with the dose of 15 Gy, and progressed slowly to the advance stage of cataract; while, for the higher dose (25 Gy), the opacity of lens appeared much earlier, and progressed more rapidly to mature stage of cataract within 1 month. At the end of the observation (90 days post-irradiation), almost all lenses became complete opacity with the higher dose (25 Gy). The degree of lens opacity was rated accordingly. The lens MDA level was increased, and SOD and GPx activities were decreased with a dose-dependent manner post-irradiation. The αB-crystallin protein level was decreased dose-dependently at the end point of observation. CONCLUSION: Oxidative events and αB-crystallin may play important roles in the pathogenesis of cataract in x-ray irradiated rat lens.

关键词： αB-crystallin malondialdehyde superoxide dismutase glutathione peroxidase x-ray irradiation cataract

Recent advances in functional nanomaterials for x-ray triggered cancer therapy

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Progress in Natural Science:Materials International 2020年第5期30卷 567-576页

作者： Teng Liu Kai Yang Zhuang Liu State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and Protection & School for Radiological and Interdisciplinary Sciences (RADX) Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University Institute of Functional Nano & Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices Soochow University

x-ray is a form of ionizing radiation with high energy and strong tissue-penetrability, which has been widely used for cancer therapy, including radiation therapy(RT) as one of the mainstream cancer treatments and radiodynamic therapy(RDT) as an emerging treatment modality. However, the nonspecific absorption of x-ray and peculiar tumor microenvironment would lead to the unsatisfied therapeutic effect and severe damage to normal tissues. The fast progress of nanotechnology has provided great opportunities for the development of functional nanoparticles and strategies to solve these problems. In this review, we will summarize the recent advances in functional nanomaterials and the related strategies, discuss how they solve the challenges in order to improve the therapeutic efficacy and safety of RT and RDT, and moreover comment on the challenges and prospects for efficient x-ray triggered cancer therapy based on nanomedicine.

关键词： x-ray irradiation Nanomaterials Cancer therapy Radiation therapy Radiodynamic therapy

irradiation facilitates the inhibitory effect of the heat shock protein 90 inhibitor NVP-BEP800 on the proliferation of malignant glioblastoma cells through attenuation of the upregulation of heat shock protein 70

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Irradiation facilitates the inhibitory effect of the heat sh...