Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells
Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells作者机构：Department of Surgery the Second Hospital of Lanzhou University Department of Ophthalmology the Second Hospital of Lanzhou University Institute of Modern Physics Chinese Academy of Sciences
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2013年第6卷第3期
页 面：286-294页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：The Foundation for Young Talents of Gansu Province China (No. 1208RJYA013)
主 题：melanoma cell 2D-LC-MS/MS stable isotope labeling with amino acids proteomic analysis X-ray irradiation protein-protein interaction
摘 要：AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein protein interaction was used to analyze relationships among proteins. RESULTS: Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. CONCLUSION: Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.
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