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Expression of JunB Induced by X-rays in Mice

Biomedical and Environmental Sciences 2004年第3期17卷 327-332页

作者： HONGWAN HIROSHIISHIHARA BeijingNeurosurgicalInstitute Beijing100050China／／BeijingNeurosurgicalInstituteTianTanXili6Bijing100050China NationalInstituteofRadiologicalSciences Chiba-shi263-8555Japan

Objective To explore JunB gene expression in spleen cells of mice after the whole body irradiation as well as in normal hematopoietic and leukemia cells in the primary culture after different dosages of X-ray irradiation. Methods Spleen cells were isolated from the mice irradiated with 3 Gy X-rays. Primary cultured cells from mice were incubated in different intervals after X-irradiation at different dosages. Total RNA was extracted from the cells and the fluctuation of JunB mRNA level was assessed by the RNA ratio of JunB/b-actin measured by quantitative Northern blot hybridization. Results After the mice were exposed to 3 Gy X-rays irradiation, JunB expression in spleen cells was remarkably and rapidly increased, and reached its peak 0.5 h later in C3H/He mice and 1 h later in Balb/c mice. In the primary culture of normal spleen and leukemia cells, JunB mRNA levels increased 30 min after irradiation. The enhanced levels of JunB mRNA were returned to a normal level within 240 min after irradiation. Conclusions JunB gene is responsive to ionizing irradiation and is induced at immediate-early phase after the stimulation. This suggests that the JunB gene plays an important role in the early process of the cells against radiation.

关键词： JunB whole body irradiation Northern blot hybridization

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