Expression of JunB Induced by X-rays in Mice

作     者：HONGWAN HIROSHIISHIHARA HONG WAN?1 AND HIROSHI ISHIHARA* Beijing Neurosurgical Institute, Beijing 100050, China;*National Institute of Radiological Sciences, Chiba-shi 263-8555, Japan

作者机构：BeijingNeurosurgicalInstituteBeijing100050China／／BeijingNeurosurgicalInstituteTianTanXili6Bijing100050China NationalInstituteofRadiologicalSciencesChiba-shi263-8555Japan 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2004年第17卷第3期

页      面：327-332页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071007[理学-遗传学] 

主　　题：JunB Whole body irradiation Northern blot hybridization 

摘      要：Objective To explore JunB gene expression in spleen cells of mice after the whole body irradiation as well as in normal hematopoietic and leukemia cells in the primary culture after different dosages of X-ray irradiation. Methods Spleen cells were isolated from the mice irradiated with 3 Gy X-rays. Primary cultured cells from mice were incubated in different intervals after X-irradiation at different dosages. Total RNA was extracted from the cells and the fluctuation of JunB mRNA level was assessed by the RNA ratio of JunB/b-actin measured by quantitative Northern blot hybridization. Results After the mice were exposed to 3 Gy X-rays irradiation, JunB expression in spleen cells was remarkably and rapidly increased, and reached its peak 0.5 h later in C3H/He mice and 1 h later in Balb/c mice. In the primary culture of normal spleen and leukemia cells, JunB mRNA levels increased 30 min after irradiation. The enhanced levels of JunB mRNA were returned to a normal level within 240 min after irradiation. Conclusions JunB gene is responsive to ionizing irradiation and is induced at immediate-early phase after the stimulation. This suggests that the JunB gene plays an important role in the early process of the cells against radiation.