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wear particle-mediated expressions of pro-inflammatory cytokines, NF-κB and RANK were impacted by lanthanum chloride in RAW264.7 cells

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Journal of Rare Earths 2013年第5期31卷 531-540页

作者：戴闽江川刘翔李哲程细高邹飏聂涛 Department of Orthopaedics The First Affiliated HospitalNanchang University Department of Orthopaedics The Second Affiliated HospitalNanchang University

To explore the impact of different concentrations of lanthanum chloride （LaC13） on critical components of wear particle-mediated signaling pathways in inflammation and osteoclastogenesis, RAW264.7 cells were naturally divided into eight groups and analyzed by CCK-8 assay, flow cytometry, ELISA, RT-PCR and western blot after treatments. The results showed that three concentrations of LaCI3 had no influence on viability of RAW264.7 cells and down-regulated receptor activator of nuclear factor rd3 （RANK） instead of macrophage colony-stimulating factor receptor （M-CSFR）. Additionally, 2.5 and 10 pmol/L LaC13 could signifi- cantly inhibit gene and protein levels of pro-inflammatory cytokines （tumor necrosis factor-or and interleukin-113, i.e., TNF-ct and IL-113） and NF-r,B/p65, but 100 pmol/L LaC13 did not exert an obvious inflammation-inhibiting effect, and even induced inflamma- tion. In conclusion, these findings demonstrated that LaC13 was able to suppress wear particle-induced inflammation and activation of NF-rd3 in a certain range of concentrations in vitro and mainly decrease the expression of RANK, but not M-CSFR, all of which were generally recognized to play a pivotal role in osteoclastogenesis.

关键词： lanthanum chloride wear particle osteoclastogenesis inflammation pro-inflammatory cytokine NF-kB RANK M-CSFR rare earths

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Dose-dependent effects of lanthanum chloride on wear particle-induced aseptic inflammation in a murine air-pouch model

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Journal of Rare Earths 2013年第4期31卷 420-427页

作者：江川邹飏刘翔尚江荫子程明戴闽 Department of Orthopaedics the First Affiliated HospitalNanchang University

To investigate the effects of local injection of different doses of lanthanum chloride （LaCl3） on aseptic inflammation in mice stimulated by wear particles from artificial joints, the particles were prepared by vacuum ball mill in vitro and air-pouch models were performed with 45 male BALB/c mice that were randomly divided into blank control group, wear particle group and wear parti- cle ＋ LaCl3 （0.1, 0.9 and 8.1 μmol） group. All animals were sacrificed and tissue specimens were harvested 7 days after treatment. Hematoxylin and eosin （H＆E） staining, enzyme-linked immunosorbent assay （ELISA）, reverse transcription-polymerase chain reac- tion （RT-PCR） and western blot were applied to observe inflammatory reaction and detect the expression of pro-inflammatory cyto- kines （TNF-et, IL-1β） and nuclear factor-κB （NF-κB） in mRNA and protein levels in air-pouch membrances. The results showed that wear particles could stimulate aseptic inflammation in vivo effectively; 0.9 μmol LaCl3 could significantly inhibit wear parti- cle-induced gene and protein expression of pro-inflammatory cytokines and NF-Id3 （P〈0.05）; 0.1 and 8. 1 μmol LaCl3 did not exert an inflammation-inhibiting effect and even caused adverse effects at 8.1 μmol. In conclusion, LaC13 played a protective role against wear particle-induced aseptic inflammation dose-dependently, which was involved in NF-κB related signaling pathways.

关键词： lanthanum chloride wear particle aseptic inflammation pro-inflammatory cytokine nuclear factor-KB air-pouch model

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EXPERIMENTAL INVESTIGATION ON THE FRACTAL BEHAVIOR OF THE wear particle ACCUMULATION

EXPERIMENTAL INVESTIGATION ON THE FRACTAL BEHAVIOR OF THE WE...

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第三届摩擦疲劳学国际会议

作者： S.R.GE H.ZHU G A. CHEN Department of Mechanical Engineering China University of Mining and Technology Xuzhou Jiangsu 221008 P. R. China

＜正＞To find a more accurate method characterizing the wear particles, the fractal geometry is applied in this paper describing the wear particle accumulation. The wear experiments are carried out on a pin-on-disc test rig under dry friction condition for copper alloy pin and steel disk. The results demonstrate that the logarithm of wear particle accumulation shows good linearity to the logarithm of measuring particle size. The relation between the accumulation of wear particles to the particle size presents a strong bi-fractal behavior. In addition, it is discovered that the fractal dimension of the wear particle accumulation changes to the different wear stages. The changes of the transition point of the bi-fractals are in correspondence to the wear state variation.

关键词： wear particle Fractal characterization particle accumulation

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