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Host Reaction of watermelon mosaic virus Isolates Infecting Melon from Different Geographical Origins in Xinjiang of China

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Horticultural Plant Journal 2017年第1期3卷 23-28页

作者： WANG Dong LI Guan College of Life Science and Technology Xinjiang University

watermelon mosaic virus(WMV) is one of the major viruses infecting cucurbit crops worldwide. Although WMV is very common worldwide,little is known about the biological traits of WMV isolates from China. Hence, this study aimed to characterize 11 WMV isolates infecting melon from different geographical origins in Xinjiang based on experimental hosts. Sap inoculation of the 11 WMV isolates onto a range of 13 plant species revealed some differences compared to the WMV isolates collected from other countries. Our results showed that, overall, there were no obvious correlations of host responses to inoculation with WMV isolates from different geographical origins. However, isolate JS-1 caused mild mosaic on Cucurbita moschata, whereas the remaining 10 isolates were asymptomatic on this plant species. Moreover, in Datura stramonium, isolate TYG-1 induced mosaic, whereas the remaining 10 isolates did not infect this species. All isolates infected systemically Cucurbita pepo and Cucumis melo plants, causing severe symptoms. All isolates did not induce any symptoms on Cucumis sativus, but the virus could be detected using RT-PCR. Additionally, all isolates infected systemically Nicotiana tabacum plants, causing mild mosaics. Chenopodium amaranticolor and Chenopodium quinoa reacted to all isolates by chlorotic local lesions in the inoculated leaves, and the virus was detected in the inoculated leaves using RT-PCR. In addition, the attempts to transmit the isolates to Luffa cylindrica, Vicia faba, Phaseolus vulgaris, Vigna unguiculata or Pisum sativum failed as confirmed by negative RT-PCR. Our results would be useful for understanding the biological variability of WMV.

关键词： melon watermelon mosaic virus host range biological traits mechanical inoculation

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Identification of watermelon mosaic virus infecting sesame in China

Identification of Watermelon mosaic virus infecting sesame i...

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中国植物病理学会2015年学术年会

作者： Y.SHI Y.J.SHI Y.Z.WANG Z.Y.WANG H.L.LI College of Plant Protection Henan Agricultural University

Seasame(Sesamum indicum) as one of the important economic crops in China is mainly distributed in Henan and Hubei province.A survey of viral diseases on sesame was carried out during the summer of 2014 in Henan province,*** the survey ten sesame fields in Zhengzhou and Zhumadian were investigated and in eight fileds mosaic,reduced leaf size,leaf deformation and leaf puckering were observed on sesame plants though the viral disease incidence was relatively low during our *** symptomatic samples were collected for virus *** RNA was extracted from symptomatic leaves and subjected to reverse transcription RT-PCR with degenerate primer pairs within the family Potyviridae,NIb2F(GTITGYGTIGAYGAYTTYAAYAA) and NIb3R(TCIACIACIGTIGAIGGYTGNCC)?(Zheng et al.2010).The expected 350-bp potyvirus-specific amplicon was observed in all symptomatic plants but not in the healthy *** amplicons were cloned and sequenced(GenBank Accession ***978924-KM978933).NCBI BLAST search showed that eight samples shared 95%9%nucleotide(nt) identity to Bean common mosaic virus strain peanut stripe isolate Laixi(GenBank Accession ***439722).The peanut stripe isolate of Bean common mosaic virus was previously reported as the pathogen causing mosaic disease in sesame(Yan et al.2009).The other two samples shared the highest nt identity of 92%and 95%to watermelon mosaic virus C04-106(WMV,GenBank Accession ***273469).WMV infection was previously reported in sesames in Korea(Chang et al.1980).During our investigation WMV infection in sesame which displayed symptoms of mosaic,dwarfing,reduced leaf size,small and reduced pods threatened sesame production in Henan *** two samples were further amplified by RT-PCR with WMV specific primer pair,wmv8249F(ACACACCAATCTTAGCGC) and wmv9798R(GACCAGTTTACCTAGTCT),which amplified a 1.5-kb product spanning 3’ half of the NIb gene to the 3’ UTR covering the full-length CP *** analyses indicated that CP g

关键词： watermelon mosaic virus identification coat protein

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A RNAi plasmid construction and application in watermelon

A RNAi plasmid construction and application in watermelon

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中国植物病理学会2015年学术年会

作者： HAO Xin-gan ZHAO Lei ZHANG Dan-dan SUN Li-ying WU Yun-feng State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A＆F University

watermelon（Citrullus lanatus） is an important crop in China with the largest planted countries in the ***,watermelon mosaic virus（WMV） is a limited factor to the watermelon planting for its huge *** other potyvirus number,WMV is also has a positive single strand RNA genome,which is bound by a viral protein genome-linked（Vpg） at the 5 terminal of *** translation initiation factors 4E（eIF4E） and its isoform（eIF（iso） 4E） were involved the process of virus infection by interacting with Vpg of ***,a RNAi plant expression vector was constructed and transmitted into watermelon to induce the gene silencing of eIF4 E and its isoform in plant.A forward 214 bp fragment was cloned from eIF4 E and a 236 bp fragment was cloned from eIF（iso） 4E,then both of them were linked as a forward fragment as 4E-iso（+）.Similar,the4E-iso（-） was cloned following the method of 4E-iso（+） *** of the 4Eiso（+） and4E-iso（-） were inserted in a RNAi plasmid of pCAMBIA1301 with a 35 S ***4E-iso（+） and 4E-iso（-） were linked with a intron of 106 bp to form a harpin RNA *** constructured RNAi plasmid was transmited into the cotyledon explants of watermelon by *** transmation,the callus induction,shoot differentiation and growth of watermelon was proceeded on the MS media with Kan and different growth *** the seedings were screened by antibiotic of Hyg,the positive transgene plants were detected by PCR method with different primer pairs and confirmed using Dot-Southern ***,we obtained four transgene plants from 157 Hyg positive plants.

关键词： watermelon mosaic virus RNAi transgene plant eIF4E

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