Identification of Watermelon mosaic virus infecting sesame in China
作者单位：College of Plant ProtectionHenan Agricultural University
会议名称：《中国植物病理学会2015年学术年会》
会议日期：2015年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
基 金：Financial support was provided by the Joint Funds of National Nature Foundation of China(U12041968)
关 键 词：Watermelon mosaic virus identification coat protein
摘 要：Seasame(Sesamum indicum) as one of the important economic crops in China is mainly distributed in Henan and Hubei province.A survey of viral diseases on sesame was carried out during the summer of 2014 in Henan province,*** the survey ten sesame fields in Zhengzhou and Zhumadian were investigated and in eight fileds mosaic,reduced leaf size,leaf deformation and leaf puckering were observed on sesame plants though the viral disease incidence was relatively low during our *** symptomatic samples were collected for virus *** RNA was extracted from symptomatic leaves and subjected to reverse transcription RT-PCR with degenerate primer pairs within the family Potyviridae,NIb2F(GTITGYGTIGAYGAYTTYAAYAA) and NIb3R(TCIACIACIGTIGAIGGYTGNCC)?(Zheng et al.2010).The expected 350-bp potyvirus-specific amplicon was observed in all symptomatic plants but not in the healthy *** amplicons were cloned and sequenced(GenBank Accession ***978924-KM978933).NCBI BLAST search showed that eight samples shared 95%9%nucleotide(nt) identity to Bean common mosaic virus strain peanut stripe isolate Laixi(GenBank Accession ***439722).The peanut stripe isolate of Bean common mosaic virus was previously reported as the pathogen causing mosaic disease in sesame(Yan et al.2009).The other two samples shared the highest nt identity of 92%and 95%to Watermelon mosaic virus C04-106(WMV,GenBank Accession ***273469).WMV infection was previously reported in sesames in Korea(Chang et al.1980).During our investigation WMV infection in sesame which displayed symptoms of mosaic,dwarfing,reduced leaf size,small and reduced pods threatened sesame production in Henan *** two samples were further amplified by RT-PCR with WMV specific primer pair,wmv8249F(ACACACCAATCTTAGCGC) and wmv9798R(GACCAGTTTACCTAGTCT),which amplified a 1.5-kb product spanning 3’ half of the NIb gene to the 3’ UTR covering the full-length CP *** analyses indicated that CP g