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Development of a High-throughput Sequencing Platform for Detection of viral encephalitis Pathogens Based on Amplicon Sequencing

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Biomedical and Environmental Sciences 2024年第3期37卷 294-302页

作者： ZHANG Ya Li SU Wen Zhe WANG Rui Chen LI Yan ZHANG Jun Feng LIU Sheng Hui HU Dan He XU Chong Xiao YIN Jia Yu YIN Qi Kai HE Ying LI Fan FU Shi Hong NIE Kai LIANG Guo Dong TAO Yong XU Song Tao MA Chao Feng WANG Huan Yu Shaanxi University of Chinese Medicine Xianyang 712000ShaanxiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Guangzhou Center for Disease Control and Prevention Guangzhou 510000GuangdongChina Shandong Center for Disease Control and Prevention Jinan 250000ShandongChina Department of Ophthalmology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Xi'an Center for Disease Control and Prevention Xi’an 710054ShaanxiChina

Objective viral encephalitis is an infectious disease severely affecting human *** is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other *** laboratory diagnosis of viral encephalitis is a worldwide ***,high-throughput sequencing technology has provided new tools for diagnosing central nervous system ***,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon *** We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant *** detection ability of the primers was verified by software simulation and the detection of known positive *** sequencing was used to validate the samples,and consistency was compared with Sanger *** The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted *** sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger *** Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral *** is also a useful tool for the high-volume screening of clinical samples.

关键词： viral encephalitis Amplicon sequencing High-throughput sequencing Multipathogen detection

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The Diagnostic Value of Oligoclonal Band Detection in viral encephalitis

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Journal of Behavioral and Brain Science 2024年第1期14卷 23-31页

作者： Anqi Huang Xianyue Meng Xueli Li^# Department of Neurology Liaocheng Hospital Affiliated to Shandong First Medical University (Liaocheng People’s Hospital) Liaocheng China

Objective: This study aims to explore the differences in cerebrospinal fluid oligoclonal band (CSF-OCB) expression among different age groups in viral encephalitis and its reference value for diagnosis. Methods: Forty-two patients with viral encephalitis were divided into two groups: 25 adults and 17 children. The presence of oligoclonal bands in the cerebrospinal fluid (CSF) was detected using polyacrylamide gel electrophoresis, and CSF routine analysis was conducted for comparative analysis. Results: The CSF-OCB positivity rate was higher in the adult group (48%) compared with the pediatric group (11.76%), with a statistically significant difference (P Conclusion: 1) The expression of CSF-OCB positivity in patients with viral encephalitis is age-related, with higher positivity rates observed in adults compared to children. 2) Although CSF oligoclonal band detection is not a specific diagnostic marker for viral encephalitis in adults, it still holds certain reference value.

关键词： Oligoclonal Band Detection viral encephalitis Routine Analysis of Cerebrospinal Fluid Age

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Advances in viral encephalitis:viral transmission,host immunity,and experimental animal models

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Zoological Research 2023年第3期44卷 525-542页

作者： Dan Yang Xiao-Jing Li De-Zhen Tu Xiu-Li Li Bin Wei State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesWuhanHubei 430071China School of Medicine Shanghai UniversityShanghai 200444China State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China Department of Cardiology Lanzhou University Second HospitalLanzhouGansu 730030China School of Life Sciences Shanghai UniversityShanghai 200444China

viral infections have led to many public health crises and pandemics in the last few *** virus infection-induced viral encephalitis(VE),especially the symptomatic inflammation of the meninges and brain parenchyma,has attracted growing attention due to its high mortality and disability *** the infectious routes of neurotropic viruses and the mechanism underlying the host immune response is critical to reduce viral spread and improve antiviral therapy *** this review,we summarize the common categories of neurotropic viruses,viral transmission routes in the body,host immune responses,and experimental animal models used for VE study to gain a deeper understanding of recent progress in the pathogenic and immunological mechanisms under neurotropic viral *** review should provide valuable resources and perspectives on how to cope with pandemic infections.

关键词： Neurotropic viruses viral encephalitis Meningeal immunity Experimental animal models

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The roles of microglia in viral encephalitis:from sensome to therapeutic targeting

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Cellular & Molecular Immunology 2021年第2期18卷 250-258页

作者： Chintan Chhatbar Marco Prinz Institute of Neuropathology Medical FacultyUniversity of FreiburgFreiburgGermany Center for Basics in NeuroModulation(NeuroModulBasics) Faculty of MedicineUniversity of FreiburgFreiburgGermany Signalling Research Centres BIOSS and CIBSS University of FreiburgFreiburgGermany

viral encephalitis is a devastating disease with high mortality,and survivors often suffer from severe neurological *** are innate immune cells of the central nervous system(CNS)parenchyma whose turnover is reliant on local *** express a diverse range of proteins,which allows them to continuously sense the environment and quickly react to *** inflammatory conditions such as CNS viral infection,microglia promote innate and adaptive immune responses to protect the ***,during viral infection,a dysregulated microglia-T-cell interplay may result in altered phagocytosis of neuronal synapses by microglia that causes neurocognitive *** this review,we summarize the current knowledge on the role of microglia in viral encephalitis,propose questions to be answered in the future and suggest possible therapeutic targets.

关键词： microglial activation viral encephalitis neurocognitive issues purinergic signaling PRR signaling

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Inhibitory effects of Shuanghuanglian injection on nuclear factor-kappa B expression in mice with viral encephalitis in a time-and dose-dependent manner

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Neural Regeneration Research 2011年第24期6卷 1865-1869页

作者： Ye Tian Caiping Han Naibing Gu Zhengli Di Gejuan Zhang Hui Lei Department of Neurology Xi＇an Center Hospital Xi＇an 710003 Shaanxi Province China

Previous studies have confirmed that the anti-virus effects of Shuanghuanglian injection may be associated with nuclear factor-kappa B activity. This study observed nuclear factor-kappa B expression in mice with viral encephalitis, and showed significant decreases in nuclear factor-kappa B protein and mRNA levels following Shuanghuanglian injection. The inhibitory effect was more significant with prolonged intervention duration and increased treatment dose. These findings verify that Shuanghuanglian injection plays a therapeutic role in viral encephalitis by reducing expression of nuclear factor-kappa B in a time- and dose-dependent manner.

关键词： nuclear factor-kappa B viral encephalitis mice gene expression Shuanghuanglian injection neural regeneration

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Clinical Efficacy and Application Progress of Reduning Injection in Children with viral encephalitis

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Medicinal Plant 2021年第2期12卷 70-73页

作者： Yuanyuan ZOU Hongying ZHANG Zhiyong XU Ziyi PAN Changsheng DENG Jianping SONG Qi WANG Guangzhou University of Chinese Medicine Guangzhou 510006 China Institute of Science and Technology Guangzhou University of Chinese Medicine Guangzhou 510006 China

viral encephalitis in children caused by multiple viral infections,as a common central nervous system disease in pediatrics,has a high incidence in *** clinical symptoms usually include infection,poisoning,intracranial hypertension,and disturbance of *** severe cases,it can even endanger the lives of patient *** present,the clinical treatment of the viral encephalitis mainly uses traditional Chinese medicine preparations such as Reduning *** efficacy is *** can not only relieve the uncomfortable symptoms in a short time,but also has low incidence of adverse *** this study,the factors that cause viral encephalitis in children were analyzed,and the efficacy,pharmacological action and clinical application of Reduning Injection were comprehensively elaborated,to provide a reference for clinical doctors to rationally treat viral encephalitis in children.

关键词： Reduning Injection Children viral encephalitis Clinical effects

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Differential transcriptomic landscapes of multiple organs from SARS-CoV-2 early infected rhesus macaques

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Protein & Cell 2022年第12期13卷 920-939页

作者： Chun-Chun Gao Man Li Wei Deng Chun-Hui Ma Yu-Sheng Chen Yong-Qiao Sun Tingfu Du Qian-Lan Liu Wen-Jie Li Bing Zhang Lihong Sun Si-Meng Liu Fengli Li Feifei Qi Yajin Qu Xinyang Ge Jiangning Liu Peng Wang Yamei Niu Zhiyong Liang Yong-Liang Zhao Bo Huang Xiao-Zhong Peng Ying Yang Chuan Qin Wei-Min Tong Yun-Gui Yang CAS Key Laboratory of Genomic and Precision Medicine Collaborative Innovation Center of Genetics and DevelopmentCollege of Future TechnologyBeijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China China National Center for Bioinformation Beijing 100101China Sino-Danish College University of Chinese Academy of SciencesBeijing 101408China Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100000China Department of Pathology Institute of Basic Medical SciencesMolecular Pathology Research CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China NHC Key Laboratory of Human Disease Comparative Medicine Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences and Comparative Medicine CenterPeking Union Medical CollegeBeijing 100021China Institute of Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeKunming 650031China Center for Experimental Animal Research Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Department of Pathology Peking Union Medical College HospitalMolecular Pathology Research CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Department of Immunology&National Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Clinical Immunology Center Chinese Academy of Medical SciencesBeijing 100005China Department of Biochemistry&Molecular Biology Tongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430030China State Key Laboratory of Medical Molecular Biology Department of Molecular Biology and BiochemistryInstitute of Basic Med

SARS-CoV-2 infection causes complicated clinical manifestations with variable multi-organ injuries,how-ever,the underlying mechanism,in particular immune responses in different organs,remains *** this study,comprehensive transcriptomic alterations of 14 tissues from rhesus macaque infected with SARS-CoV-2 were *** to normal controls,SARS-CoV-2 infection resulted in dysregulation of genes involving diverse functions in various examined tissues/organs,with drastic transcriptomic changes in cerebral cortex and right ***,cerebral cortex exhibited a hyperinflammatory state evidenced by sig-nificant upregulation of inflammation response-related ***,expressions of coagulation,angio-genesis and fibrosis factors were also up-regulated in cerebral *** on our findings,neuropilin 1(NRP1),a receptor of SARS-CoV-2,was significantly elevated in cerebral cortex post infection,accompanied by active immune response releasing inflammatory factors and signal transmission among tissues,which enhanced infection of the central nervous system(CNS)in a positive feedback way,leading to viral ***,our study depicts a multi-tissue/organ tran-scriptomic landscapes of rhesus macaque with early infection of SARS-CoV-2,and provides important insights into the mechanistic basis for COVID-19-asso-ciated clinical complications.

关键词： SARS-CoV-2 NRP1 inflammation central nervous system viral encephalitis rhesus macaque

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肺吸虫病误诊一例

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临床放射学杂志 1999年第8期18卷 57-57页

作者：汪新亚湖北省黄石纺织机械厂卫生所放射科

患者女，３１岁。心慌气短４天伴右季肋区不适，钝痛，活动及深呼吸时为甚，无发热，二便可。查体：咽部稍红肿，双肺呼吸音稍粗。心率９９次／分，律齐。腹软，右肋下腋前线交点处压痛。Ｂ超：肝、胆、脾、双肾无异常回声。右胸腔内可... 详细信息

患者女，３１岁。心慌气短４天伴右季肋区不适，钝痛，活动及深呼吸时为甚，无发热，二便可。查体：咽部稍红肿，双肺呼吸音稍粗。心率９９次／分，律齐。腹软，右肋下腋前线交点处压痛。Ｂ超：肝、胆、脾、双肾无异常回声。右胸腔内可见液性暗区。Ｘ线检查：右胸第４前肋...

关键词： viral encephalitis brain potential rehabilitation

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