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Mosquito densovirus signifcantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes(Diptera:Culicidae)

引用

Infectious Diseases of Poverty 2023年第3期12卷 91-92页

作者： Ling Kong Jie Xiao Lu Yang Yuan Sui Duoquan Wang Shaoqiang Chen Peiwen Liu Xiao-Guang Chen Jinbao Gu Guangdong Provincial Key Laboratory of Tropical Disease Research Depart-ment of Pathogen BiologySchool of Public HealthSouthern Medical Uni-versityGuangzhou 510515GuangdongChina Brown School Washington UniversitySt.LouisMO 63130USA National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionShanghai 200025China Shenzhen Aiming Pest Control Operation Service Company Limited Shenz-henGuangdongChina

Background Dengue virus(DENV)is a major public health threat,with Aedes albopictus being the confrmed vector responsible for dengue epidemics in Guangzhou,*** densoviruses(MDVs)are pathogenic mosquitospecifc viruses,and a novel MDV was previously isolated from *** in *** study aims to determine the prevalence of MDVs in wild *** populations and investigate their potential interactions with DENV and impact on vector susceptibility for *** The prevalence of MDV in wild mosquitoes in China was investigated using open access sequencing data and PCR detection in *** in *** viral infection rate and titers in MDV-persistent C6/36 cells were evaluated at 12,24,48,72,96,and 120 h post infection(hpi)by indirect immunofuorescence assay(IFA)and real time quantitative PCR(RT-qPCR).The midgut infection rate(MIR),dissemination rate(DR),and salivary gland infection rate(SGIR)in various tissues of MDV-infected mosquitoes were detected and quantifed at 0,5,10,and 15 days post infection(dpi)by RT-PCR and *** chi-square test evaluated dengue virus serotype 2(DENV-2)and Aedes aegypti densovirus(AaeDV)infection rates and related indices in mosquitoes,while Tukey’s LSD and t-tests compared viral titers in C6/36 cells and tissues over *** The results revealed a relatively wide distribution of MDVs in Aedes,Culex,and Anopheles mosquitoes in China and an over 68%positive *** vitro,signifcant reductions in DENV-2 titers in supernatant at 120 hpi,and an appar‑ent decrease in DENV-2-positive cells at 96 and 120 hpi were *** vivo,DENV-2 in the ovaries and salivary glands was frst detected at 10 dpi in both monoinfected and superinfected *** females,while MDV super‑infection with DENV-2 suppressed the salivary gland infection rate at 15 ***-2 titer in the ovary and salivary glands of *** was reduced in superinfected mosquitoes at 15 *** MDVs is widespread in nat

关键词： DENV-2 Mosquito densovirus Superinfection Aedes albopictus vector susceptibility

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