Mosquito densovirus signifcantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes(Diptera:Culicidae)

作     者：Ling Kong Jie Xiao Lu Yang Yuan Sui Duoquan Wang Shaoqiang Chen Peiwen Liu Xiao-Guang Chen Jinbao Gu 

作者机构：Guangdong Provincial Key Laboratory of Tropical Disease ResearchDepart-ment of Pathogen BiologySchool of Public HealthSouthern Medical Uni-versityGuangzhou 510515GuangdongChina Brown SchoolWashington UniversitySt.LouisMO 63130USA National Institute of Parasitic DiseasesChinese Center for Disease Control and PreventionShanghai 200025China Shenzhen Aiming Pest Control Operation Service Company LimitedShenz-henGuangdongChina 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2023年第12卷第3期

页      面：91-92页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

主　　题：DENV-2 Mosquito densovirus Superinfection Aedes albopictus Vector susceptibility 

摘      要：Background Dengue virus(DENV)is a major public health threat,with Aedes albopictus being the confrmed vector responsible for dengue epidemics in Guangzhou,*** densoviruses(MDVs)are pathogenic mosquitospecifc viruses,and a novel MDV was previously isolated from *** in *** study aims to determine the prevalence of MDVs in wild *** populations and investigate their potential interactions with DENV and impact on vector susceptibility for *** The prevalence of MDV in wild mosquitoes in China was investigated using open access sequencing data and PCR detection in *** in *** viral infection rate and titers in MDV-persistent C6/36 cells were evaluated at 12,24,48,72,96,and 120 h post infection(hpi)by indirect immunofuorescence assay(IFA)and real time quantitative PCR(RT-qPCR).The midgut infection rate(MIR),dissemination rate(DR),and salivary gland infection rate(SGIR)in various tissues of MDV-infected mosquitoes were detected and quantifed at 0,5,10,and 15 days post infection(dpi)by RT-PCR and *** chi-square test evaluated dengue virus serotype 2(DENV-2)and Aedes aegypti densovirus(AaeDV)infection rates and related indices in mosquitoes,while Tukey’s LSD and t-tests compared viral titers in C6/36 cells and tissues over *** The results revealed a relatively wide distribution of MDVs in Aedes,Culex,and Anopheles mosquitoes in China and an over 68%positive *** vitro,signifcant reductions in DENV-2 titers in supernatant at 120 hpi,and an appar‑ent decrease in DENV-2-positive cells at 96 and 120 hpi were *** vivo,DENV-2 in the ovaries and salivary glands was frst detected at 10 dpi in both monoinfected and superinfected *** females,while MDV super‑infection with DENV-2 suppressed the salivary gland infection rate at 15 ***-2 titer in the ovary and salivary glands of *** was reduced in superinfected mosquitoes at 15 *** MDVs is widespread in nat