检索结果-南通市图书馆

Partial recovery of disturbed v-j pairing profiles of T-cell receptor in people living with HIv receiving long-term antiretroviral therapy

引用

Science China(Life Sciences) 2021年第1期64卷 152-161页

作者： Guoli Li jiarui Li Henghui Zhang Yu Zhang Di Liu Yu Hao junyan Han juan Du Liuluan Zhu Yongqin Zeng Bei Li Rui Li Chuan Song Fujie Zhang Chen Chen Hongxin Zhao Hui Zeng Institute of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing100015China Beijing Key Laboratory of Emerging Infectious Diseases Beijing100015China Clinical and Research Center of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing100015China Computational virology Group Center for Bacteria and Virus Resources and ApplicationWuhan Institute of VirologyChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing101408China

Chronic human immunodeficiency virus(HIv) infection not only causes a gradual loss of CD4~+T cells but also leads to a disturbance of the T cell receptor(TCR) repertoire. In people living with HIv(PLWH), monitoring TCR repertoire is challenged by the inconsistency of complementarity determining region 3(CDR3) and limited cell numbers in clinical samples. Thus, a quantitative method is necessary for monitoring the TCR repertoire in *** characterized the TCR v-j pairing profile of na?ve and memory CD4~+T cells in healthy donors, HIv-infected antiretroviral therapy(ART)-na?ve patients and long-term(over5 years) ART-experienced patients by performing TCR sequencing. We developed a v-j index with 18 parameters which were subdivided into five categories(expression coverage, cumulative percentage of the top tenth percentile, diversity, intra-individual similarity and inter-individual similarity). In ART-na?ve patients, 14 of the 18 parameters were significantly ***-term ART recovered ten parameters. The four unrecovered parameters were related to inter-individual ***, these findings indicate that long-term ART could only partially recover TCR v-j pairs and introduce newly impacted v-j pairs. Moreover, these results provide new insights into the v-j pairing of the TCR and into the disturbance of TCR repertoire in HIv infection.

关键词： acquired immune deficiency syndrome human immunodeficiency virus antiretroviral therapy T cell receptor v-j pairing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains

引用

Protein & Cell 2014年第8期5卷 603-615页

作者： Peipei Liu Di Liu Xi Yang jing Gaoai Ma3, Fangqing Zhaos, Xuyu Zhou~'2~, George F. Gao1'2'4'5~, Baoli Zhu~'2~ Yan Chen Xue Xiao Fei Liu jing Zou jun Wu juncai Ma Fangqing Zhao Xuyu Zhou George F. Gao Baoli Zhu CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Network Information Center Institute of Microbioiogy Chinese Academy of Sciences Beijing 100101 China Chinese Center for Disease Control and Prevention (China CDC) Beijing 102206 China Research Network of Immunity and Health (RNIH) Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101 China

The characterization of the human T-cell receptor （TCR） repertoire has made remarkable progress, with most of the work focusing on the TCRβ chains. Here, we ana- lyzed the diversity and complexity of both the TCRa and TCRβ repertoires of three healthy donors. We found that the diversity of the TCRα repertoire is higher than that of the TCRβ repertoire, whereas the usages of the v and j genes tended to be preferential with similar TRAv and TRAj patterns in all three donors. The v-j pairings, like the v and j gene usages, were slightly preferential. We also found that the TRDv1 gene rearranges with the majority of TRAj genes, suggesting that TRDv1 is a shared TRAv/Dv gene （TRAv42/Dv1）. Moreover, we uncovered the presence of tandem TRBD （TRB D gene） usage in -2% of the productive human TCRβ CDR3 sequences.

关键词： TCR repertoire next-generationsequencing v/j usage v-j pairing CDR3 D-D fusion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：