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A surface-modified biodegradable urethral scaffold seeded with urethral epithelial cells

Chinese Medical Journal 2011年第19期124卷 3087-3092页

作者： FU Wei-jun WANG Zhong-xin LI Gang ZHANG Bing-hong ZHANG Lei HU Kun HONG Bao-fa WANG Xiao-xiong CUI Fu-zhai ZHANG Xu Department of Urology Chinese People＇s Liberation Army General Hospital Beijing 100853 China Biomaterials Lab School of Materials Science and Engineering Tsinghua University Beijing 100084 China

Background Efficient cell adhesion and proliferation is a central issue in cell-based tissue engineering, which offers great promise for repair of urethral defects or strictures. This study evaluated the adhesion and growth of rabbit uroepithelium on a surface-modified three-dimensional poly-L-lactic acid （PLLA） *** urethral mucosa were harvested from male New Zealand rabbits and the urothelium were dissociated and then cultured. Immunocytochemistry on cultured uroepithelium for pancytokeratin and uroplakin Ⅱ and TE-7 confirmed pure populations. After in vitro proliferation, cells were seeded onto a surface-modified urethral scaffold with non-knitted filaments. The morphology and viability of the cells were examined by immunohistochemical and fluorescence *** and scanning microscopes were used to document cell growth and *** Three to five days after primary culture, the uroepithelial cells gradually became confluent, assuming a cobblestone pattern. The filaments of the urethral scaffold had excellent biocompatibility and allowed growth of the uroepithelium, without affecting viability. The uroepithelial cells adhered to and grew well on the scaffold. After 3-7 days,the cells grew vigorously and meshes of the scaffold were full of *** The surface-modified urethral scaffold with non-knitted filaments allows the growth of uroepithelium and can serve as a carrier for the tissue engineering of urethra.

关键词： biodegradation urethral scaffold urothelium cell adhesion tissue engineering

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Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure urethral Tubular scaffold

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Chemical Research in Chinese Universities 2012年第5期28卷 912-915页

作者： WANG Xiao-qing CHEN Qi-hui HOU Yu-chuan LU Zhi-hua HU Jing-hai ZHANG Hai-feng HAO Yuan-yuan ZHANG Long GAO Zhan-tuan WANG Chun-xi Department of Urology the First Hospital of Jilin UniversityChangchun 130021P.R.China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022P.R.China

A degradable poly（lactic-co-glycolic acid, LA：GA=80：20）（PLGA） urethral tubular scaffold was fabricated by electrospinning. In order to enhance the mechanical properties, the scaffold was crosslinked with glutaraldehyde. The structure and properties of the crosslinked scaffolds were investigated by the mechanical property testing, scanning electron microscopy（SEM）, degradability test in vitro and 3-（4,5）-dimethylthiahiazo（-z-yl）-3,5-diphenytetrazo- liumromide（MTT）. The results show that the scaffold has the nano-structure. The pore size and the porosity are suitable for cell seeding, growth and extracellular matrix production. Although influenced by the crosslinking slightly, the pore size and the porosity could still support cell proliferation and tissuse formation. The mechanical properties are remarkably increased by the crosslinking of glutaraldehyde, and it could meet the demands of a urethral stent. The scaffold could completely collapse within 70 d. The results of the biocompatibility test show that the PLGA scaffold had no cytotoxicity.

关键词： Poly(lactic-co-glycolic acid) urethral scaffold Degradable Electrospin Crosslinking

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