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Rapid development of double-hit mRNA antibody cocktail against orthopoxviruses

Signal Transduction and Targeted Therapy 2024年第4期9卷 1768-1776页

作者： Hang Chi Suo-Qun Zhao Ru-Yi Chen Xing-Xing Suo Rong-Rong Zhang Wen-Hui Yang Dong-Sheng Zhou Min Fang Bo Ying Yong-Qiang Deng Cheng-Feng Qin State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAMMS100071BeijingChina College of Life Sciences Inner Mongolia Agricultural UniversityHohhot010018Inner MongoliaChina School of Life Sciences Henan UniversityKaifeng475004HenanChina Suzhou Abogen Biosciences Co. LtdSuzhou215123JiangsuChina Research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical Sciences100071BeijingChina

The Orthopoxvirus genus,especially variola virus(VARV),monkeypox virus(MPXV),remains a significant public health threat worldwide.The development of therapeutic antibodies against orthopoxviruses is largely hampered by the high cost of antibody engineering and manufacturing processes.mRNA-encoded antibodies have emerged as a powerful and universal platform for rapid antibody production.Herein,by using the established lipid nanoparticle(LNP)-encapsulated mRNA platform,we constructed four mRNA combinations that encode monoclonal antibodies with broad neutralization activities against orthopoxviruses.In vivo characterization demonstrated that a single intravenous injection of each LNP-encapsulated mRNA antibody in mice resulted in the rapid production of neutralizing antibodies.More importantly,mRNA antibody treatments showed significant protection from weight loss and mortality in the vaccinia virus(VACV)lethal challenge mouse model,and a unique mRNA antibody cocktail,Mix2a,exhibited superior in vivo protection by targeting both intracellular mature virus(IMV)-form and extracellular enveloped virus(EEV)-form viruses.In summary,our results demonstrate the proof-of-concept production of orthopoxvirus antibodies via the LNP-mRNA platform,highlighting the great potential of tailored mRNA antibody combinations as a universal strategy to combat orthopoxvirus as well as otheremerging viruses.

关键词： proof universal neutral

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In search of a pan-coronavirus vaccine:next-generation vaccine design and immune mechanisms

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Cellular & Molecular Immunology 2024年第2期21卷 103-118页

作者： S.Cankat M.U.Demael L.Swadling Division of Infection and Immunity Institute of Immunity and TransplantationUniversity College LondonPears BuildingLondon NW32PPUK

Members of the coronaviridae family are endemic to human populations and have caused several epidemics and pandemics in recent history.In this review,we will discuss the feasibility of and progress toward the ultimate goal of creating a pan-coronavirus vaccine that can protect against infection and disease by all members of the coronavirus family.We will detail the unmet clinical need associated with the continued transmission of SARS-CoV-2,MERS-CoV and the four seasonal coronaviruses(HCoV-OC43,NL63,HKU1 and 229E)in humans and the potential for future zoonotic coronaviruses.We will highlight how first-generation SARS-CoV-2 vaccines and natural history studies have greatly increased our understanding of effective antiviral immunity to coronaviruses and have informed next-generation vaccine design.We will then consider the ideal properties of a pan-coronavirus vaccine and propose a blueprint for the type of immunity that may offer cross-protection.Finally,we will describe a subset of the diverse technologies and novel approaches being pursued with the goal of developing broadly or universally protective vaccines for coronaviruses.

关键词： Vaccine Coronavirus Cross-reactive immunity Cross-protection universal

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universal and one-step visualization of latent fingermarks onvarious surfaces using hydrophilic cellulose membrane and dye aqueous solution

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Science China Chemistry 2017年第9期60卷 1250-1257页

作者： Qianhui Wei Xiaolong Li Xin Du Xueji Zhang Meiqin Zhang Research Center for Bioengineering&Sensing Technology University of Science&Technology BeoingBeijing 100083China

A high-resolution and universal method for the rapid visualization of latent fingermarks on a broad variety of surfaces has been achieved by simply combining hydrophilic cellulose membrane with dye aqueous solution.In this approach,the relatively hydrophobic characteristic of fingermark residue enable the deposit act as a "mask",directing dye absorption processes to regions of both furrows without the ridge residues and bare cellulose membrane substrate.This effect during the spatially selective dye absorption generates a negative pattern of the fingermark within a few seconds to minutes depending on the properties of the substrates and fingermark types.It provides observation of latent fingermarks on cellulose membrane surface with high definition of level 2 and level 3 details used for personal identification purposes.It is also highly-efficient for visualizing the latent fingermarks lifted from various common(glass,carton,ceramic cup) and problematic(banknote,human skin and leather) surfaces by using transparent adhesive tape.To examine the generality of the proposed method,eight different kinds of dye aqueous solutions have been tested for developing latent fingermarks and they all provide very good results as expected.Moreover,this approach is easily used for aged fingermarks and natural fingermarks as well.Imaging may be accomplished visibly(by dye color) and topographically(using a microscope or a camera).This approach is a simple,rapid,non-destructive,safe,low-cost,universal and high resolution method compared with conventional approaches,so it demonstrates good potentiality in individual identity validation related applications.

关键词： latent fingermarks visualization, cellulose membrane, dye solution, universal and high resolution, various substrates

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A universal IN TAILING WAVETRAIN GENERATION

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Journal of Hydrodynamics 2004年第5期16卷 603-607页

作者： XUZhao-ting SAMUELS.P.Shen QIAOFang-li InstituteofPhysicalOceanographyandPhysicalOceanographyLaboratory OceanUniversityofChinaQingdao266003China DepartmentofMathematicsScience UniversityofAlbertaEdmontonT6G2G1Canada KeyLaboratoryofMarineScienceandNumericalModeling TheFirstInstituteofOceanographyStateOceanicAdministrationQingdao266061China

A universal in tailing wave-train generation of forced soliton generationover topography is found theoretically as the flows are at the resonant points and it is examinedwith the numerical calculation of the corresponding fKdV e-quatioa From the comparisons, it is shownthat theoretical and numerical results on the invariance is in good agreement and the theory givenin this paper does not include the modulus truncation, any free constant and unknown function.

关键词： universal soliton tailing wave-train fKdV equation KdV equations

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universal Expression of Efficiency at Maximum Power:A Quantum-Mechanical Brayton Engine Working with a Single Particle Confined in a Power-Law Trap

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Communications in Theoretical Physics 2015年第12期64卷 671-675页

作者：叶卓林李卫胜赖益铭何济洲王建辉 Department of Physics Nanchang University State Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of Sciences

We propose a quantum-mechanical Brayton engine model that works between two superposed states,employing a single particle confined in an arbitrary power-law trap as the working substance. Applying the superposition principle,we obtain the explicit expressions of the power and efficiency,and find that the efficiency at maximum power is bounded from above by the function: η+= θ/(θ+1),with θ being a potential-dependent exponent.

关键词： quantum-mechanical Brayton engine universal expres

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Identification of a Novel universal Potential Epitope on the Cytoplasmic Tail of H7N9 Virus Hemagglutinin

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Virologica Sinica 2019年第3期34卷 334-337页

作者： Xi Liu Li Ding Jing Yuan Jian Liao Lian Duan Wenfei Wang Weiguo Tan Weiye Yu Boping Zhou Xinchun Chen Zheng Yang Department of Infectious Diseases The Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 51900China Lab for Tuberculosis Research Shenzhen Center for Chronic Disease ControlShenzhen 518020China Department of Infectious Diseases The Third People's Hospital of Shenzhen (The Second Affiliated Hospital of Southern University of Science and Technology)Shenzhen 518000China Shenzhen Key Laboratory of Infection and Immunity The Third People's Hospital of Shenzhen (The Second Affiliated Hospital of Southern University of Science and Technology)Shenzhen 518000China Department of Pathogen Biology School of MedicineShenzhen UniversityShenzhen 518000China Longhua Branch of Shenzhen People's Hospital Shenzhen 518000China

Dear Editor,H7N9 is a recently identified subtype of influenza A virus that caused a major outbreak in humans in China in 2013.According to the latest data provided by the Chinese Center for Disease Control and Prevention(http://***/zwgk/yqbb3/ejlist.shtml,updated on October 31,2018),the mortality rate of H7N9 infections in China amounts to 39.7%(611/1536).Thus,H7N9 poses a serious public health threat.

关键词： Identification Novel universal Potential Epitope Cytoplasmic Tail

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Development of Genomic Microsatellite Multiplex PCR Using Dye-Labeled universal Primer and Its Validation in Pedigree Analysis of Pacific Oyster(Crassostrea gigas)

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Journal of Ocean University of China 2017年第1期16卷 151-160页

作者： LIU Ting LI Qi SONG Junlin YU Hong Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003P.R.China

There is an increasing requirement for traceability of aquaculture products, both for consumer protection and for food safety. There are high error rates in the conventional traceability systems depending on physical labels. Genetic traceability technique depending on DNA-based tracking system can overcome this problem. Genealogy information is essential for genetic traceability, and microsatellite DNA marker is a good choice for pedigree analysis. As increasing genotyping throughput of microsatellites, microsatellite multiplex PCR has become a fast and cost-effective technique. As a commercially important cultured aquatic species, Pacific oyster Crassostrea gigas has the highest global production. The objective of this study was to develop microsatellite multiplex PCR panels with dye-labeled universal primer for pedigree analysis in C. gigas, and these multiplex PCRs were validated using 12 full-sib families with known pedigrees. Here we developed six informative multiplex PCRs using 18 genomic microsatellites in C. gigas. Each multiplex panel contained a single universal primer M13(-21) used as a tail on each locus-specific forward primer and a single universal primer M13(-21) labeled with fluorophores. The polymorphisms of the markers were moderate, with an average of 10.3 alleles per locus and average polymorphic information content of 0.740. The observed heterozygosity per locus ranged from 0.492 to 0.822. Cervus simulations revealed that the six panels would still be of great value when massive families were analysed. Pedigree analysis of real offspring demonstrated that 100% of the offspring were unambiguously allocated to their parents when two multiplex PCRs were used. The six sets of multiplex PCRs can be an important tool for tracing cultured individuals, population genetic analysis, and selective breeding program in C. gigas.

关键词： Crassostrea gigas traceability microsatellites universal primer multiplex PCR pedigree analysis

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On universal approximation capability of fuzzy systems

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Science China(Technological Sciences) 1998年第1期41卷 6-12页

作者：毛志宏张雪枫李衍达 Department of Automation Tsinghua University Beijing China

The universal approximation capability of fuzzy systems using translations and dilations of one fixed function (called basis function) as their membership functions is discussed. Such types of fuzzy systems are proved to be universal approximators under conditions that the basis function is integrable, with nonvanishing integral, and a.e. continuous. This result enlarges the family of fuzzy systems which can be universal approximators. Two simulation experiments are designed to verify the conclusion.

关键词： fuzzy systems membership functions universal approximators.

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A universal theory of measure and integral on valuation spaces with respect to diverse implication operators

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Science China(Technological Sciences) 2000年第6期43卷 586-594页

作者：王国俊 Institute of Mathematics Shaanxi Normal University Xi'an 710062 China

Valuation spaces with respect to diverse implication operators are investigated in a unified way where the Lebesgue measure is a commonly used measure, and it is proved that all the logic formulas are measurable functions with respect to popularly used implication operations. The concept of t-(α-tautology) is introduced and rules of generalized modus ponens (MP) and hypothetic syllogism (HS) are established in the sense of semantics. The concept of truth degree of a logic formula is introduced and rules of integral MP and integral HS are proposed. Finally, a kind of pseudo-metric is introduced to the set consisting of all logic formulas by establishing a universal logical metric space, making it possible to develop a new type of approximate reasoning arise.

关键词： implication operator valuation space Lebesgue measure and integral t(α-tautology) truth de-gree universal logical metric space approximate reasoning.

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universal INTEGRABLE REPRESENTATIONS FOR KAC-MOODY ALGEBRAS

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Chinese Science Bulletin 1991年第6期36卷 445-448页

作者：张贺春 Institute of Systems Science Academia Sinica Beijing 100080 PRC

Ⅰ. INTRODUCTIONIntegrable representation is an important concept in the theory of Kac-Moody algebras. In this note, we study a class of integrable modules which are called universal integrable modules.

关键词： integrable module universal integrable module.

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