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ultrastructural observations of The Anterior Chamber Angle Tissues in Congenital Glaucoma

眼科学报 1994年第1期10卷 50-56,49页

作者： Jinyong Lin, Wei Sun, Enjiang Li, Sihui Wang,Jingwen Bai, Haixian YangTianjin Eye Hospital, Tianjin 300040, China Tianjin Medical Cellege, Tianjin 300000, China Tianjin Eye Hospital China.

Eighteen trabeculectomy specimens of congenital glaucoma were examined by light and transimssion electron microscopy. The results showed that the primary anomalies in congenital glaucoma included the developmental defects of trabecular meshwork, excessive collagen fibrils in the trabecular matrix, shifting forward of ciliary muscle fibres and persistent mesenchymal tissues in the anterior-chamber angle. The authors also pointed out the importance of the secondary lesions of the trabecular meshwork in th...

关键词： congenital glaucoma ultrastructural observation pathogenesis

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A novel cell line established from skin tissue of gibel carp(Carassius gibelio)is susceptible to Carassius auratus herpesvirus infection

Water Biology and Security

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Water Biology and Security 2024年第2期3卷 62-72页

作者： Qi-Qi Jiang Zhi Li Qi-Ya Zhang Fei Ke Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture The Innovation Academy of Seed DesignInstitute of HydrobiologyChinese Academy of SciencesWuhan430072China University of Chinese Academy of Sciences Beijing100049China

Frequent outbreaks of emerging infectious diseases in fish,such as Carassius auratus herpesvirus(CaHV)infection has caused great economic losses in ***,the lack of a sensitive cell culture system has limited studies of *** the present study,a new cell line(gibel carp skin cell,GiCS)derived from gibel carp(Carassius gibelio)skin tissue was established to create a valuable tool for research of the *** GiCS cells consisted mainly of epithelial-like cells,which grew well at 25℃in L-15 medium supplemented with 10–20%fetal bovine *** analysis revealed that the skin cell line remained amphitriploid,with most chromosome counts being 156(54%).The GiCS cells can be efficiently transfected and expressed exogenous *** particular,the GiCS cells showed high susceptibility to CaHV infection,which was confirmed by virus infection tests,detection of viral gene expression,and ultrastructural *** our knowledge,it is the first cell line that is highly permissive to CaHV *** addition,the cells also showed susceptibility to several aquatic animal viruses from different families including Iridoviridae,Rhabdoviridae,and *** conclusion,these results indicated that the establishment of the GiCS cell line is a significant advance that will be beneficial to future studies of CaHV and other aquatic animal viruses.

关键词： Gibel carp Skin cell line Virus susceptibility Carassius auratus herpesvirus ultrastructural observation

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Effect of Sodium Selenite on Pathological Changes and Renal Functions in Broilers Fed a Diet containing Aflatoxin B1

Effect of Sodium Selenite on Pathological Changes and Renal ...

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第七届中国畜牧科技论坛

作者： Liang na Wang fengyuan Peng xi Fang jing Cui hengmin Chen zhengli Lai weimin Zhou yi Geng yi Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province College of Veterinary MedicineSichuan Agricultural University

To evaluate the renal toxicity of dietary aflatoxin B(AFB) and ameliorating effects of added dietary sodium selenite in broiler,renal histopathological changes,ultrastructural changes and renal function parameters were monitored at 7,14,and 21 days of *** hundred one-day-old healthy male Avian broilers were divided into four groups,namely control group,AFB group(0.3 mg/kg AFB),+Se group(0.4 mg/kg Se) and AFB +Se group(0.3 mg/kg AFB +0.4 mg/kg Se).Compared with that of the control group,the relative weight of kidney was increased in the AFB *** were no significant differences between the AFB +Se group and the control *** histopathological observation,the renal epithelia were swelling and necrosis at 7 and 21 days of ***,the lipid droplets and expanded endoplasmic reticulum appeared in the plasma of epithelia cells in the *** mitochondria with degenerated cristae were observed in the+Se *** with the control group,the contents of serum creatinine and serum uric acid in the AFBgroup were increased,while the activity of renal Na-K ATPase was *** 0.4mg/kg selenium was added into the diet containing 0.3 mg/kg AFB,there were no obvious histological changes in the AFB +Se group,and the contents of the serum creatinine and serum uric acid contents and the activity of renal Na-K ATPase were close to those in the control *** conclusion,sodium selenite exhibited protective effects on AFB-induced kidney toxicity in broilers.

关键词： Aflatoxin B1 Sodium Selenite Pathological observation ultrastructural observation Renal Function Broiler

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