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Chinese Journal of Structural Chemistry 2020年第6期39卷 1157-1166页

作者： SHI Jian-Cheng HUANG Xiao-Qian LUO Min HUANG Chu-Sheng College of Chemistry and Material Nanning Normal UniversiyNanning 530001China

The three-dimensional quantitative structure-activity relationships(3D-QSAR)for 37 curcumin derivatives were constructed by CoMFA and CoMSIA methods,*** results showed that the cross validated coefficient(q^2)and non-cross-validated coefficient(R^2)were 0.711,0.962 in CoMFA model and 0.774,0.856 in CoMSIA model,respectively,which suggests that two models are robust and have good exterior predictive *** on these two models and the binding mode with tubulin,nine novel curcuminoids inhibitors which could exhibit much higher anticancer potency and efficiently occupy the colchicine binding site of tubulin,were *** expect that the results in this paper have the potential to facilitate the process of design and to develop new potent curcumin derivatives with stronger anticancer activities.

关键词： anticancer activity tubulin curcumin derivatives 3D-QSAR molecular docking molecular design

Electrochemical Studies of Paclitaxel Interaction with tubulin

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Chinese Chemical Letters 2000年第4期11卷 351-352页

作者： Yong YU Qi Long LI (Department of Chemistry, Beijing Normal Normal University. Beijing 100873) Beijing Normal Univ Dept Chem Beijing 100875 Peoples R China

A highly sensitive linear sweep voltammetric method was developed for the determination of paclitaxel and the mechanism of the binding of paclitaxel to tubulin was studied. tubulin dimer formed with paclitaxel an electrochemically nonactive complex with a combination ratio of 2:2. Its stability constant was 2.85 x 10(22). So the tubulin dimer had two binding sites for paclitaxel. The experiment showed that the binding sites of paclitaxel to tubulin dimer were different from that of Ca2+ to tubulin dimer.

关键词： paclitaxel tubulin linear sweep voltammetry

DSC Study on Brain tubulin and the Effect of Cisplatin

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Chinese Chemical Letters 2000年第3期11卷 243-246页

作者： Xiao Lei ZHOU Yong YANG Bao Huai WANG Zhi Fen LI Ai Xiao LIU Lung Fei YEN Institute of Physical Chemistry Peking UniversityBeijing 100871 College of Biological Science China Agricultural UniversityBeijing 100094

The thermal property of the polymerization of brain tubulin was studied by a high-sensitivity differential scanning calorimeter. The phenomenon that heat flows increased and decreased consistently and obviously was observed. This phenomenon was called heat flow oscillation. It was probably correlated to the dynamic instability of microtubules., The effect of cisplatin on it was reported, too.

关键词： tubulin cisplatin DSC heat flow oscillation

In vitro assembly of plant tubulin in the absence of microtubule-stabilizing reagents

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Chinese Science Bulletin 2000年第24期45卷 2258-2263页

作者： HUANG Shanjin REN Haiyun YUAN Ming Key Laboratory of Plant Physiology and Biochemistry of Ministry of Agriculture Department of Plant SciencesCollege of Biological SciencesChina Agricultural UniversityBeijing 100094China Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life SciencesBeijing Normal UniversityBeijing 100875China

The assembly of microtubules is essential for physiological functions of microtubules. Addition of microtubule-stabilizing reagents or microtubule 'seeds' is usually necessary for plant tubulin assembly in vitro, which hinders the investigation of plant microtubule dynamics. In the present note, highly purified plant tubulins have been obtained from lily pollen, a non-microtubule-stabilizing reagent or microtubule 'seed' system for plant tubulin assembly has been established and the analysis of plant tubulin assembly performed. Experiment results showed that purified tubulin polymerized in vitro, and a typical microtubule structure was observed with electron microscopy. The kinetics curve of tubulin assembly exhibited typical 'parabola'. The presence of taxol significantly altered the character of plant tubulin assembly, including that abnormal microtubules were assembled and the critical concentration for plant tubulin assembly was decreased exceedingly from 3 mg/mL in the absence of taxol to 0.043 mg/

关键词： lily pollen tubulin microtubule tubulin assembly.

Posttranslational modifications of α-tubulin in alzheimer disease

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Translational Neurodegeneration 2015年第1期4卷 59-67页

作者： Fan Zhang Bo Su Chunyu Wang Sandra L.Siedlak Siddhartha Mondragon-Rodriguez Hyoung-gon Lee Xinglong Wang George Perry Xiongwei Zhu Department of Pathology Case Western Reserve UniversityClevelandOH 44121USA Department of Neurosurgery Shandong Provincial HospitalShandong UniversityJinan 250012China Department of Neurobiology Shandong UniversityJinan 250012China Department of Neurology the Second Xiangya HospitalCentral South UniversityChangshaHunan 410011China Departamento de Neurobiología del Desarrollo y Neurofisiología Instituto de NeurobiologíaUniversidad Nacional Autónoma de México QuerétaroQuerétaro MéxicoD.F.Mexico The University of Texas at San Antonio One UTSA CircleSan AntonioTX 78249USA 2103 Cornell Road ClevelandOH 44106USA

Background:In Alzheimer disease(AD),hyperphosphorylation of tau proteins results in microtubule destabilization and cytoskeletal *** prior ultra-morphometric studies documented a clear reduction in microtubules in pyramidal neurons in AD compared to controls,however,this reduction did not coincide with the presence of paired helical *** latter suggests the presence of compensatory mechanism(s)that stabilize microtubule dynamics despite the loss of tau binding and *** are composed of tubulin dimers which are subject to posttranslational modifications that affect the stability and function of ***:In this study,we performed a detailed analysis on changes in the posttranslational modifications in tubulin in postmortem human brain tissues from AD patients and age-matched controls by immunoblot and ***:Consistent with our previous study,we found decreased levels ofα-tubulin in AD *** of tubulin with various posttranslational modifications such as polyglutamylation,tyrosination,and detyrosination were also proportionally reduced in AD brain,but,interestingly,there was an increase in the proportion of the acetylatedα-tubulin in the remainingα-*** distribution was changed from predominantly in the processes to be more accumulated in the cell *** number of processes containing polyglutamylated tubulin was well preserved in AD *** there was a cell autonomous detrimental effect of NFTs on tubulin,this is likely a gradual and slow process,and there was no selective loss of acetylated or polyglutamylated tubulin in NFT-bearing ***:Overall,we suggest that the specific changes in tubulin modification in AD brain likely represent a compensatory response.

关键词： Acetylation Alzheimer disease Polyglutamylation Tau tubulin

Expression pattern of a β_2 tubulin gene in zebrafish embryos identified by whole mount in situ hybridization

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Chinese Science Bulletin 1998年第22期43卷 1894-1898页

作者： LI Shuhong, GUO Shaodong and SUN Fangzhen Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China Institute of Developmental Biology Chinese Academy of Sciences Beijing China

Zebrafish is a good vertebrate model for development studies. Research on gene expression and regulation during zebrafish embryonic development plays an important role in revealing the molecular mechanism of morphogenesis in vertebrate. tubulin locates the cytoplasmic determinants in eggs, organizes the formation of basic cytoskeleton and controls various cell movements. There are several types of tubulin, each of which has specific expression regions and functions. In this study, the full_length cDNA of a Xenopus β 2 tubulin was used as template to generate Dig_labeled antisense RNA which was used as a probe to carry out in situ hybridization on whole mount zebrafish embryos. Under the experimental conditions, the tubulin transcript was first detected in a group of cells in embryos at the midblastula stage. With the development of the embryo, this transcript was gradually restricted to the nervous system. These results demonstrate that the expression of this β 2 tubulin gene in zebrafish embryos is neural specific.

关键词： tubulin gene whole mount in situ hybridization zebrafish.

Expression of α tubulin Gene in Motoneurons of Spinal Cord During Nerve Regeneration

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Chinese Science Bulletin 1993年第12期38卷 1038-1042页

作者：何彬范明刘淑红甘思德 Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing 100850 PRC

We have previously reported that the injury of sciatic nerve causes not only the change of axonal ultrastructures, but also the increase of β tubulin gene expression in the neural soma. And the microtubule, as a carrier of transported materials, of axonal architecture plays an important role. It has also been found that the content and transported velocity of tubulin in the regeneration nerve increase significantly owing to the requirements of axonal reconstruction. As the NOR-Ag staining technique is specific

关键词： motoneuron NOR-Ag tubulin gene in situ hybridization.

Identification and expression analysis of tubulin gene family in upland cotton

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Journal of Cotton Research 2021年第3期4卷 229-238页

作者： CHEN Baojun ZHAO Junjie FU Guoyong PEI Xinxin PAN Zhao'e LI Hongge AHMED Haris HE Shoupu DU Xiongming State Key Laboratory of Cott on Biology Institute of Cotton ResearchChinese Academy of Agricultural Sciences(ICRCAAS)Anyang 455000China

Background:Cott on fibers are single-celled exte nsions of the seed epidermis,a model tissue for studying *** genes play a critical role in synthesizing the microtubules(MT)as a core element of the ***,there is a lack of studies concerning the systematic characterization of the tubulin gene family in ***,the identification and portrayal of *** tubulin genes can provide key targets for molecular manipulation in cotton ***:In this study,we investigated all tubulin genes from different plant species and identified 98 tubulin genes in *** an a lysis showed that tubulin family genes were classified into three *** protein motifs and gene structure ofβ-tubulin genes are more conserved compared withγ-tubulin *** tubulin genes are located at the proximate ends of the *** expression pattern by transcriptome and qRT-PCR analysis revealed that 12α-tubulin andβ-tubulin genes are specifically expressed during different fiber development ***,Gh.A03G027200,Gh.D03G 169300,and Gh.A1lG258900 had differential expression patterns at distinct stages of fiber development in varieties JO2508 and ***:In this study,the evol ut io nary an alysis showed that the tubulin genes were divided into three *** genetic structures and molecular functions were highly con served in different *** candidate genes,Gh.A03G027200f Gh.D03G169300,and Gh.A11G258900 may play a key role during fiber development complementing fiber length and strength.

关键词： Upland cotton Fiber quality Cytoskeleton Microtubules(MT) tubulin

EFFECT OF QINGYANGSHEN ON HIPPOCAMPAL α-AND β-tubulin GENE EXPRESSION DURING KAINIC ACID INDUCED EPILEPTOGENESIS

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Journal of Traditional Chinese Medicine 1993年第4期13卷 281-286页

作者：郭庆匡培根 Neurotransmitter Laboratory Department of NeurologyChinese PLA General HospitalPLA Postgraduate Medical SchoolBeijing 100853China

Using α-and β-tubulin cDNA Probes and Northern blot hybridization technique,we ana-lyzed the effect of intraperitoneally injected Qingyangshen (QYS),a traditional Chinesemedicine with antiepileptic property,and diphenylhydantoin sodium (DPH) on hippocampalα-and β-tubulin gene expression during kainic acid (KA) induced chronic *** wasfound that:1) thirty days after intraperitoneal injection of KA,α-and β-tubulin mRNAs inanimals showing chronic seizures increased 3.02±1.05 and 4.07±1.32 times respectivelycompared with control;2) neither QYS (15 mg/kg,q.o.d.×6) nor DPH (50 mg/kg,q.o.d.×6),when used separately,could inhibit the above mentioned effect of KA;3) whenQYS (15 mg/kg,q.o.d.×6) and DPH (50 mg/kg,q.o.d.×6) were given in combination,the long-term increase in tubuliu gene expression induced by KA was significantly reduced,with α-and β-tubulin mRNAs being decreased to 0.44±0.08 and 0.50×0.10 times of corresponding values in animals treated with KA *** results indicate that the mechanismof antiepileptic effect of QYS is at least partially related to the inhibition of tubulin synthe-sis and subsequent reduction in mossy fiber sprouting and neosynaptogensis.

关键词： seizures tubulin sprouting hippocampal alone subsequent inhibit AND injected partially

条斑紫菜tubulin和Kinesin基因家族鉴定及失水胁迫表达模式分析

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海洋与湖沼 2021年第3期52卷 673-684页

作者：崔正彩孔凡娜茅云翔孙斌王俊皓董道英中国海洋大学海洋生命学院海洋生物遗传学与育种教育部重点实验室青岛266003 海南热带海洋学院水产与生命学院热带海洋生物资源利用与保护教育部重点实验室三亚572022

微管是细胞骨架的主要成分,其结构及动力学机制对提高生物体耐受性具有重要作用。微管网络如何通过结构的动态变化调控适应环境变化已成为胁迫生物学领域的研究热点之一。条斑紫菜(Pyropia yezoensis)能够适应潮间带复杂多变的环境,是... 详细信息

微管是细胞骨架的主要成分,其结构及动力学机制对提高生物体耐受性具有重要作用。微管网络如何通过结构的动态变化调控适应环境变化已成为胁迫生物学领域的研究热点之一。条斑紫菜(Pyropia yezoensis)能够适应潮间带复杂多变的环境,是研究潮间带大型海藻抗逆机制的良好材料。目前对紫菜微管相关基因家族构成及其生物学功能的研究较少。本研究利用生物信息学方法,在条斑紫菜基因组中共鉴定出4个tubulin基因(Pyα-tubulin 1、Pyα-tubulin 2、Pyβ-tubulin和Pyγ-tubulin)和11个Kinesin基因(PyKinesin 1—PyKinesin 11),并对其理化性质、基因结构、蛋白特征、染色体定位、系统进化和失水胁迫下的表达模式进行了系统分析。结果显示:条斑紫菜中有3种微管蛋白(tubulin)亚型;该家族成员散布于1号和2号染色体上,Pyα-tubulin 1和Pyα-tubulin 2为串联重复基因;Pytubulin家族成员在基因结构和蛋白特征方面均较为保守,且在转录水平对失水胁迫不敏感。条斑紫菜中有5种驱动蛋白(Kinesin)亚型,亚家族种类和基因数量均少于高等植物;该家族基因散布于1号、2号和3号染色体上,无串联重复基因;PyKinesin家族成员在基因结构和蛋白特征方面存在一定差异,PyKinesin 1在中高度失水胁迫下表达量显著上调。本研究为进一步理解tubulin和Kinesin基因家族的进化和功能、解析微管在条斑紫菜响应失水胁迫中的作用奠定了基础。

关键词：条斑紫菜 tubulin Kinesin 基因家族失水胁迫