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tributyrin administration improves intestinal development and health in pre-weaned dairy calves fed milk replacer

Animal Nutrition 2022年第3期10卷 399-411页

作者： Shuai Liu Junda Wu Zhaohai Wu Gibson Maswayi Alugongo Muhammad Zahoor Khan Jinghui Li Jianxin Xiao Zhiyuan He Yulin Ma Shengli Li Zhijun Cao State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Institute of Animal Husbandry and Veterinary Guizhou Academy of Agricultural SciencesGuizhou 550005China State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China Department of Animal Science University of CaliforniaDavisCA 95616USA Faculty of Veterinary and Animal Sciences University of AgricultureDera Ismail Khan29220Pakistan

Butyrate and its derivatives possess various nutritional and biological benefits for mammals,whereas its effects on dairy calves have not been well *** study evaluated the effects of tributyrin administration on blood immune,intestinal immune and barrier functions,and microbial composition of pre-weaned dairy *** newborn Holstein bull calves were randomly assigned into a control group(no tributyrin supplementation,CON;n=10)or a treatment group(supplemented with tributyrin at 2 g/L of milk,TRB;n=10).The results showed that diarrhea frequency was decreased significantly by tributyrin administration from d 29 to 56(Ptributyrin administration significantly reduced the concentration of interleukin-1b(IL-1b)on d 28(P=0.001)and tended to reduce the concentration of serum amyloid A on d 56(P=0.079),whereas serum oxidative status parameters were not *** intestinal development,tributyrin administration increased the villus height(Ptributyrin *** the ileum,claudin-4(P=0.029)and G-protein coupled receptor 41(P=0.019)gene expressions were upregulated in the TRB group compared to *** significantly higher abundances of microbiota were found in the jejunum or ileum of calves in the CON *** the TRB group,supplementing tributyrin significantly increased the abundance of shortchain fatty acid(SCFA)-producing bacteria,including Ruminococcaceae,Lachnospiraceae,Prevotella and Rikenellaceae(LDA>3.5,P<0.05),which was negatively associated with inflammatory gene expression(TLR2 and IL-1b)but positively associ

关键词： Dairy calves tributyrin Gut microbiota Inflammation Barrier function

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Effects of dietary tributyrin on intestinal mucosa development,mitochondrial function and AMPK-mTOR pathway in weaned pigs

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Journal of Animal Science and Biotechnology 2020年第2期11卷 602-610页

作者： Chunchun Wang Shuting Cao Zhuojun Shen Qihua Hong Jie Feng Yan Peng Caihong Hu Key Laboratory of Molecular Animal Nutrition Ministry of EducationAnimal Science CollegeZhejiang UniversityYu Hang Tang Rd No.866Hangzhou 310058People’s Republic of China Shanghai Menon Animal Nutrition Technology Co.Ltd. Shanghai 201807China

Background:The objective of this experiment was to investigate the influence of dietary tributyrin on intestinal mucosa development,oxidative stress,mitochondrial function and AMPK-mTOR signaling ***:Seventy-two pigs were divided into two treatments and received either a basal diet or the same diet supplemented with 750 mg/kg *** treatment has six replicates of six *** 14 days,6 pigs from each treatment were selected and the jejunal samples were ***:Results showed that supplemental tributyrin increased(Ptributyrin had greater(Ptributyrin *** tributyrin supplementation lowered(Ptributyrin increased(Ptributyrin elevated(Ptributyrin promotes intestinal mucosa growth,extenuates oxidative stress,improves mitochondrial function and modulates the AMPK-mTOR signal pathway of weaned pigs.

关键词： AMPK-mTOR signaling pathway Intestinal mucosa development Mitochondrial function tributyrin Weaned pigs

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Benefits of tributyrin on growth performance,gastrointestinal tract development,ruminal bacteria and volatile fatty acid formation of weaned Small-Tailed Han lambs

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Animal Nutrition 2023年第4期15卷 187-196页

作者： Zhiwei Li Xueer Wang Wei Wang Ran An Yaxin Wang Qingchang Ren Jingjing Xuan College of Animal Science Anhui Science and Technology UniversityFengyang233100China College of Animal Science and Technology Tarim UniversityAlae843300China Anhui Province Key Laboratory of Animal Nutritional Regulation and Health Anhui Science and Technology UniversityFengyang233100China School of Finance and Economics Anhui Science and Technology UniversityBengbu233030China

This study aimed to determine the effects of tributyrin on growth performance,gastrointestinal tract development,ruminal bacteria and volatile fatty acid(VFA)*** healthy weaned SmallTailed Han female lambs at 3 months old with BW 27.5±4.1 kg(mean±SD)were randomly assigned to five groups of six lambs each,and each group received tributyrin at 0,0.5,1.0,2.0 and 4.0 g/kg in *** were measured before the start and end of the *** 15 d adaptation,DMI,feed,faeces and urine were recorded every *** were sacrificed at d *** to lambs fed no tributyrin,lambs fed 4.0 g/kg tributyrin had higher average daily BW gain(P=0.04)and DMI(Ptributyrin reduced nitrogen(Ptributyrin increased dorsal sac thickness(Ptributyrin increased thicknesses of both the duodenum(Ptributyrin decreased duodenal(Ptributyrin reduced rumen pH(Ptributyrin improved the structure of rumen bacteria by enhancing Clostridium(P=0.04),Butyrivibrio(Ptributyrin could stimulate gastrointestinal tract development by enhancing colonization of rumen VFA-producing bacteria,and dietary supplementation of tributyrin at 4.0 g/kg of DM was recommended for the weaned lambs.

关键词： tributyrin Bacteria Gastrointestinal tract Growth Weaned lamb

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Digestion performance improvement of tributyrin through nano-emulsion preparation technology

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Grain & Oil Science and Technology 2022年第3期5卷 131-145页

作者： Feng Gao Yaoming Cui Shijia Song Jinfeng Zhu Xinyu Liu Xuyang Ji Hao Zhu Qingyu Jia Qiaohan Lin Junjun Guan College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

The current research aimed to optimize the preparation technology of tributyrin nano-emulsion(TBNE)to improve its digestion properties, which were investigated through digestion models in vitro and in vivo. The content of each component of TBNE was optimized by response surface methodology(RSM) to improve the z-average particle size and stability of TBNE. The optimized TBNE was evaluated for its digestion properties by in vitro and in vivo models. The preparation conditions of TBNE optimized by RSM were as follows: 61.3% of sorbitol, 32.7% of tributyrin, and 6.0% of modified phospholipid(MP). The predicted z-average particle size of TBNE was(246.02 ± 18.10) nm. The results of the verification test showed that the z-average particle size, zeta potential, conductivity, emulsification activity index, and emulsification stability index of TBNE were(250.02 ± 7.18) nm,(-40.23 ± 0.76) m V,(31.80 ± 2.09) μS/cm,(848.00 ± 84.53) min and(1.14 ± 0.02) m^(2)/g, respectively. The in vitro digestion experiment results showed that the TBNE remained stable in the stomach and was released in the intestine, while the size of TBNE in the gastrointestinal tact was significantly smaller than that of tributyrin(P tributyrin treatment, TBNE significantly promoted the average body weight at the 7th day, average daily feed intake,average daily gain, feed/gain, ileum weight, and organ index of ileum of Hy-Line BROWN chicks(P tributyrin treatment were172.18 mg/m L and 100.85 mg/m L(P tributyrin.

关键词： tributyrin Nano-emulsion Response surface methodology Digestion property Chick

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Enhancement of liposomal stability and cellular drug uptake by incorporating tributyrin into celecoxib-loaded liposomes

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Asian Journal of Pharmaceutical Sciences 2013年第2期8卷 128-133页

作者： Sung Nam Kang Soon-Seok Hong Soo-Yeon Kim Heungchan Oh Mi-Kyung Lee Soo-Jeong Lim Department of Bioscience and Biotechnology Sejong University98 Kunja-dongKwangjin-guSeoul 143-747Republic of Korea Hanil High School GongjuChungchungnam-Do 314-714Republic of Korea College of Pharmacy Woosuk UniversityWanju-gunJeollabuk-Do 565-701Republic of Korea

tributyrin,a triglyceride analog of short chain fatty acid butyrate,can act as a prodrug of an anticancer agent *** view of other reports that demonstrated the improved characteristics of conventional liposomes by incorporating small amount of nonphospholipids such as Tween 80,herein we sought to investigate whether the incorporation of tributyrin into the liposomal layers may provide additional advantages for liposomes as an anticancer drug *** were prepared with dimyristoylphosphatidylcholine as a main phospholipid with or without addition of *** was loaded in liposomes as a model anticancer *** 80-incorporated liposomes were also prepared for ***-incorporated liposomes were ineffective in enhancing the skin permeation of celecoxib compared to Tween 80-incorporated ***,tributyrin-incorporated liposomes enhanced the entrapped celecoxib concentration to an extent comparable to Tween 80-incorporated ***,tributyrin-incorporated liposomes exhibited much higher stability compared to Tween 80-incorporated ***,the cellular uptake of celecoxib loaded in tributyrin-incorporated liposomes into mouse melanoma cells were more than 10-fold higher compared to that loaded in conventional-and Tween 80-incorporated *** together,the incorporation of tributyrin into conventional liposomes loaded with anticancer drugs may provide an advanced anticancer drug carrier delivering both drug and tributyrin.

关键词： tributyrin Liposomes Celecoxib Cancer Formulation

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tributyrin in the Diet of Lambs and Its Impacts on Energy Metabolism and Oxidative Status in the Liver and Intestine, as Well as the Fatty Acid Profile in Meat

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Food and Nutrition Sciences 2024年第11期15卷 1065-1084页

作者： Ana Iris S. dos Santos Julcemar D. Kessler Bruna Klein Andrei L. R. Brunetto Guilherme L. Deolindo Luiz Eduardo L. Silva Roger Wagner Poliheny Martins da Silva Matheus D. Baldissera Aleksandro S. da Silva Programa de Ps-graduao Zootecnia Universidade do Estado de Santa Catarina (UDESC) Chapec Brasil Departamento de Zootecnia Universidade do Estado de Santa Catarina (UDESC) Chapec Brasil Programa de Ps-graduao Multicntrico em Bioqumica e Biologia Molecular Universidade do Estado de Santa Catarina (UDESC) Lages Brasil Departamento de Cincia de Alimentos Universidade Federal de Santa Maria Santa Maria Brasil Laboratrio de Bioprospeco e Biologia Experimental Universidade Franciscana (UFN) Santa Maria Brasil

This study aimed to determine whether adding tributyrin to the diet of lambs impacts health, energy metabolism, ruminal environment, and meat quality. Twelve lambs were used;the control group received a basal diet, while the tributyrin group received a diet with the additive mixed with concentrate (2 g/day/animal). The blood count was used for Leukocyte and lymphocyte counts, which were significantly higher in tributyrin-fed animals than in controls. The activity of the enzymes adenylate kinase and pyruvate kinase was higher in the liver and intestine of the tributyrin group than the controls;cytosolic creatine kinase activity was significantly higher in the intestine of lambs fed tributyrin. Glutathione S-transferase activity in the liver was significantly higher in animals in the tributyrin group. Superoxide dismutase activity was significantly higher in the intestine, with a lower protein carbonyl concentration in the tributyrin group. Bacterial activity through ruminal fluid collection was significantly lower when tributyrin was consumed, unlike the protozoan count, which was significantly higher in animals in the tributyrin group than in the controls. tributyrin intake caused lower levels of short-chain fatty acids without changing the proportion of volatile fatty acids. The water retention capacity measured using an external compression method was significantly higher in the meat of the tributyrin group. The treatment affected some fatty acids in the meat, these acids were separated by chromatography where a lower amount of saturated fatty acids and a higher amount of monounsaturated fatty acids in the group that consumed tributyrin. These findings suggest that tributyrin in lamb diet alters blood and rumen environment biomarkers and improves the fatty acid profile of the meat.

关键词： Additive Animal Production tributyrin Fatty Acids Animal Health

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tributyrin通过诱导细胞凋亡和抑制DNA合成而抑制人胃癌SGC-7901细胞的生长

Tributyrin通过诱导细胞凋亡和抑制DNA合成而抑制人胃癌SGC-7901...

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中国细胞生物学学会医学细胞生物学、免疫细胞生物学和发育生物学专业委员会学术研讨会

作者：严俊徐永华中国科学院上海生命科学研究院生物化学与细胞生物学研究所

为研究天然中性短链脂肪酸甘油三酯、丁酸前体药物tributyrin对人胃癌细胞是否存在生长抑制作用,在人胃癌细胞SGC-7901中,用0.5,1,2.5,10 and 5OmmolL tributyrin处理,并用MTT方法检测其对细胞生长的作用。74kBq的[H]-TdR加入细胞培液,... 详细信息

为研究天然中性短链脂肪酸甘油三酯、丁酸前体药物tributyrin对人胃癌细胞是否存在生长抑制作用,在人胃癌细胞SGC-7901中,用0.5,1,2.5,10 and 5OmmolL tributyrin处理,并用MTT方法检测其对细胞生长的作用。74kBq的[H]-TdR加入细胞培液,利用[H]-TdR的掺入率来观察DNA合成情况。电镜和Hoechst-33258染色观察细胞凋亡的形态学。利用流式细胞分析和末端脱氧核苷酸转移酶介导的dUIP缺口末端标记法（TUNEL）检测tributyrin诱导的细胞凋亡。利用免疫印迹方法检测*** Bax蛋白改变表达。tributyrin能够引起SGC-7901细胞的生长抑制和细胞凋亡,并呈现剂量和时间依赖性。在tributyrin处理48小时后,SGC-7901细胞内Bcl-2蛋白水平下调,而Bax蛋白的表达量上升,同时伴有PARP的剪切。tributyrin体外能够有效地通过抑制DNA合成和诱导细胞凋亡达到抑制胃癌细胞生长的作用,细胞凋亡的作用与Bcl-2表达量下调和Bax表达量上升有关。因此,tributyrin可能是一个非常有潜力的化学预防和化学治疗人胃癌形成的药物。

关键词： tributyrin 胃癌细胞凋亡 DNA合成

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三丁酸甘油酯诱导白血病细胞分化及凋亡机制的研究

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中国实验血液学杂志 2005年第5期13卷 774-777页

作者：殷红陈子兴岑建农王玮段卫明姚利苏州大学附属第一医院

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin,TB)诱导NB4、K562白血病细胞分化和(或)凋亡的作用机制,利用Westernblot方法及逆转录聚合酶链反应(RTPCR)检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的... 详细信息

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin,TB)诱导NB4、K562白血病细胞分化和(或)凋亡的作用机制,利用Westernblot方法及逆转录聚合酶链反应(RTPCR)检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。结果表明:NB4(或K562)细胞经TB0.1mmol/L(或TB0.5mmol/L)作用16小时后可见组蛋白H3乙酰化水平明显上升,并有剂量依赖效应。NB4、K562细胞经TB0.1mmol/L作用后可见p21WAF1mRNA表达上调,且具有剂量依赖效应。p21WAF1mRNA的这种上调开始于TB作用2小时后,16小时达高峰,可维持48小时。结论:TB诱导NB4、K562细胞发生分化和(或)凋亡的机制与TB引起细胞组蛋白高乙酰化和继而上调p21WAF1表达有关。

关键词： tributyrin NB4 K562 组蛋白乙酰化 p21^WAF1

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Combination of all trans retinoic acid with butyric acid and its prodrugs markedly enhancing differentiation of human acute promyelocytic leukemia NB 4 cells

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Chinese Medical Journal 1999年第4期 65-68页

作者：陈子兴王玮潘金兰陈丽娟薛永权

Objective To use NB 4, an authentic human acute promyelocytic leukemia cell line, as well as the marrow cells from patients with acute promyelocytic leukemia (APL), containing the PML/RARα fusion gene and fused protein to examine the growth inhibition and cytodifferentiation induced by all trans retinoic acid (ATRA), butyric acid (BA) and its prodrug tributyrin (TB) either as a single agent or in *** NB 4 and APL cells were cultured in presence of ATRA, BA and TB respectively either as a single agent or in combinations at various concentration ratio. Cell growth was measured and myeloid differentiation was determined by morphology and the percentage of positive nitroblue tetrazolium reduction (NBT) on consecutive days over the whole process of culture. Results NB 4 cells can be induced by ATRA alone and synergistically induced by the combinations of BA or TB with ATRA to differentiate. The synergy was reflected by a remarkable decrease in the effective concentration of ATRA required in the combinations in comparison with it as a sole agent. The combinations also shortened the time for the cells to reach the same level of maturation as that needed for ATRA alone. The potentiation on ATRA induced differentiation of NB 4 cells seemed depending on an appropriate concentration ratio of each inducer in the combinations and the time of action. A preliminary result of in vitro induction of primarily cultured leukemic cells from APL patients by the combined inducers was promising. Conclusion The combinations of ATRA with BA or TB at an appropriate ratio may improve the clinical outcome of differentiation therapy for APL patients.

关键词： acute promyelocytic leukemia · all trans retinoic acid · butyric acid · tributyrin · differentiation

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三丁酸甘油酯诱导白血病细胞增殖抑制、分化、凋亡及其机理的研究

三丁酸甘油酯诱导白血病细胞增殖抑制、分化、凋亡及其机理的研究

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作者：殷红苏州大学

学位级别：硕士

目的：探讨三丁酸甘油酯（tributyrin，TB）对人急性白血病细胞株NB4、K562、SHI-1以及白血病原代培养细胞的增殖抑制、诱导分化、凋亡作用及其可能的作用机制。方法：利用台盼蓝拒染法，MTT比色法、细胞形态学观察，NB... 详细信息

目的：探讨三丁酸甘油酯（tributyrin，TB）对人急性白血病细胞株NB4、K562、SHI-1以及白血病原代培养细胞的增殖抑制、诱导分化、凋亡作用及其可能的作用机制。方法：利用台盼蓝拒染法，MTT比色法、细胞形态学观察，NBT还原率，联苯胺染色法，检测细胞表面抗原CD11b、CD14、CD71表达，流式细胞仪测定AnnexinV结合力和DNA含量，DNA凝胶电泳等方法观察TB对白血病细胞K562、NB4、SHI-1及18例白血病原代培养细胞的增殖抑制、诱导分化及凋亡作用。通过Western blotting方法检测组蛋白H3乙酰化水平的改变。用逆转录聚合酶链反应（RT-PCR）方法测定TB作用于白血病细胞后，细胞周期蛋白依赖性激酶抑制物p21WAF1／CIP1表达量的改变。结果：①TB可以抑制K562、NB4、SHI-1细胞的增殖及活力，呈剂量-时间依赖效应。TB0.5mM作用72h后，原代培养的白血病细胞及正常骨髓细胞存活率分别为56.3±20.7％、95.7±2.8％，两者间有显著差别（p＜0.01）。TB作用后16小时，K562、NB4、SHI-1细胞阻滞于G1或／和G2期，并伴分布于S期细胞明显下降，因药物浓度及细胞株的不同而有所差异。②K562、NB4、SHI-1及原代培养的白血病细胞经TB0.5-1.0mM作用48小时后，出现典型的凋亡形态学改变。TB处理后K562、NB4、SHI-1细胞AnnexinV结合力明显上升，并可见典型的DNA梯带，提示细胞发生凋亡。③TB0.1mM／ATRA0.05uM联合作用于NB4细胞，细胞形态学及NBT还原率均提示细胞出现分化。TB0.1mM作用后SHI-1 三]一酸甘油醋诱导白血病细胞增殖抑制、分化、凋亡及其机理的研究中文提要细胞形态上出现部分分化，NBT阳性率升高，细胞表面CDllb、CD14 表达上调。TB 0.2mM单用及联合Al…RA ***作用于K562细胞，细胞形态学、联苯胺染色法、细胞表面CD71检测均未提示细胞出现分化。 TB ***/ATRA ***联合作用于AML一Ml来源的原代细胞后，形态学可见部分分化。④TB处理Ks62、sHI一1、NB4细胞后，Wcstern blotting 法可测得乙酞化组蛋白H3水平升高。TB作用2小时即有组蛋白乙酞化水平的上升，可维持24小时。⑤TB处理后Ks62、NB4、SHI一i细胞 P21WA““C‘P’表达均明显上调，并具有剂量一时间依赖性。结论:①TB能抑制K562、NB4、SHI一1细胞增殖并影响细胞活力;阻滞细胞周期进程于Gl和/或GZ期。②TB能抑制白血病原代培养细胞的增殖，而对正常骨髓细胞无明显影响。③TB单用或联合ATRA可诱导K562、 NB4、SHI一1及白血病原代培养细胞的分化和/或凋亡，作用因药物浓度及细胞株的不同而有所差异。④TB抑制白血病细胞增殖，诱导分化和/ 或凋亡作用与TB引起组蛋白高乙酞化，继而上调pZIWAF‘表达及有关。

关键词： tributyrin 白血病细胞株分化凋亡 p21WAF1 组蛋白乙酰化

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