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Ribosome Profiling Reveals Genome-wide Cellular translational regulation upon Heat Stress in Escherichia coli

Genomics, Proteomics & Bioinformatics 2017年第5期15卷 324-330页

作者： yanqing zhang zhengtao xiao qin zou jianhuo fang qifan wang xuerui yang ning gao MOE Key Laboratory of Protein Sciences Beij'ing Advanced Innovation Center for Structural BiologyTsinghua—Peking Center for Lt Sciences School 0f LIfe SciencesTsinghua University.Beijing 100084 China MOE Key Laboratory of Bioinformatics.Tsinghua-Peking Joint Center for ife Sciences.Center for Synthetic and Systems Biology School of Lire SciencesTsinghua University Beijing 100084China State Key Laboratory ofMembrane Biology Peking—Tsinghua Centerfor Lire Sciences.School of Life Sciences.Peking University Beijing 100871Chin

Heat shock response is a classical stress-induced regulatory system in bacteria, character- ized by extensive transcriptional reprogramming. To compare the impact of heat stress on the tran- scriptome and translatome in Escherich＆ coli, we conducted ribosome profiling in parallel with RNA-Seq to investigate the alterations in transcription and translation efficiency when E. coli cells were exposed to a mild heat stress （from 30 ~C to 45 ~C）. While general changes in ribosome foot- prints correlate with the changes of mRNA transcripts upon heat stress, a number of genes show differential changes at the transcription and translation levels. translation efficiency of a few genes that are related to environment stimulus response is up-regulated, and in contrast, some genes func- tioning in mRNA translation and amino acid biosynthesis are down-regulated at the translation level in response to heat stress. Moreover, our ribosome occupancy data suggest that in generalribosomes accumulate remarkably in the starting regions of ORFs upon heat stress. This study pro- vides additional insights into bacterial gene expression in response to heat stress, and suggests the presence of stress-induced but yet-to-be characterized cellular regulatory mechanisms of gene expression at translation level.

关键词： Ribosome profiling translation regulation RNA-Seq Heat shock response Transcription regulation

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translational regulation of DNA repair systems by eIF3a in cancer chemotherapeutic response

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中国药理学与毒理学杂志 2016年第10期30卷 1050-1051页

作者： CHEN Juan CUI Jia-jia ZHANG Jian-ting ZHOU Hong-hao LIU Zhao-qian YIN Ji-ye Department of Clinical Pharmacology Xiangya HospitalCentral South University Institute of Clinical Pharmacology Central South University Hunan Key Laboratory of Pharmacogenetics Department of Pharmacology and Toxicology Indiana University School of Medicine IU Simon Cancer Center Indiana University School of Medicine

OBJECTIVE To investigate the role of e IF3a in the regulation of DNA repair pathways in cancer chemotherapeutic *** Immunohistochemistry was used to determine the expression of e IF3a in lung and breast cancer tissues followed by association analysis of e IF3a expression with patient′s response to *** overexpression and RNA interference knockdown of e IF3a were carried out in NIH3T3and H1299 cell lines,respectively,to determine the effect of altered e IF3a expression on cellular response to chemotherapeutic drugs by using MTT *** DNA repair capacity of these cells was evaluated by using host-cell reactivation,NHEJ and HR ***-time reverse transcriptase PCR and Western Blot analyses were carried out to determine the effect of e IF3a on the DNA repair genes by using cells with altered e IF3a *** e IF3a expression associates with response of lung and breast cancer patients to platinum and anthracycline.e IF3a knockdown or overexpression,respectively,increased and decreased the cellular resistance to cisplatin and anthracycline anticancer drugs,DNA repair activity,and expression of NER and NHEJ DNA repair *** e IF3a plays an important role in regulating the expression of NER and NHEJ DNA repair proteins which,in turn,contributes to cellular response to DNA-damaging anticancer drugs and patients′response to platinum and anthracycline chemotherapy.

关键词： eIF3a DNA repair translation regulation platinum anthracycline cancer chemotherapy

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