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Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat

World Journal of Gastroenterology 2005年第31期11卷 4788-4793页

作者： Mei-Na Shi Wei-Da Zheng Li-Juan Zhang Zhi-Xin Chen Xiao-Zhong Wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To study the effect of IL-10 on the expression of growth factors - transforming growth factor-β1 （TGF-β1）, epidermal growth factor （EGF）, hepatocyte growth factor （HGF）and platelet-derived growth factor （PDGF） of hepatic stellate cells （HSCs） of hepatic fibrosis rat and the anti-fibrogenic role of exogenous IL-10. METHODS： Hepatic fibrosis was induced by CCI4 administration intra-peritoneally. Sixty clean male Sprague-Dawley （SD） rats were randomly divided into three groups： normal control group （GN, 8 rats）, hepatic fibrosis model group （GC, 28 rats） and IL-10 treated group （GI, 24 rats）. At the beginning of the 7^th and 11^th wk, rats in each group were routinely perfused with pronase E and type IV collagenase through a portal vein catheter and the suspension obtained from the liver was spun by centrifugation with 11% Nycodenz density gradient to isolate HSCs. Histological examination was used to determine the degree of hepatic fibrosis. RT-PCR was employed to analyze mRNA expression from freshly isolated cells. Immunocytochemistry was performed to detect protein expression in primary cultured HSCs. RESULTS： Rat hepatic fibrosis was developed with the increase of injection frequency of CCl4, and HSCs were successfully isolated. At the 7^th and 11^th wk, TGF-β1, EGF, and HGF mRNA in GC increased obviously compared with GN （P = 0.001/0.042, 0.001/0.001, 0.001/0.001） and GI （P= 0.001/0.007, 0.002/0.001, 0.001/0.001）. For TGF-β1, no difference was observed between GI and GN. For EGF, mRNA level in GI increased compared with GN during the 7^th wk （P= 0.005） and 11^th wk （P= 0.049）. For HGF, mRNA level in GI decreased compared with GN at the 7^th wk （P = 0.001） and 11^th wk （P= 0.021）. Between these two time points, TGF-β1 expression at the 7^th wk was higher than that of the 11^th wk （P = 0.049）, but for EGF, the former was lower than the latter （P = 0.022）. As for PDGF mRNA, there was no significant difference between thesegroups, but difference seemed to exist i

关键词： Hepatic fibrosis Hepatic stellate cells Interleukin-10 transforming growth factor-131 Epidermal growth factor Hepatocyte growth factor Platelet-derived growth factor

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Effects of Ginkgo biloba on prevention of development of experimental diabetic nephropathy in rats

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Acta Pharmacologica Sinica 2007年第6期28卷 818-828页

作者： Qian LU~2,Xiao-xing YIN~(2,3),Jian-yun WANG~2,Yuan-yuan GAO~2,Ying-mei PAN~2~2 Department of Pharmacy,Xuzhou Medical College,Xuzhou 221002,China Department of Pharmacy Xuzhou Medical College Xuzhou China

Aim:To observe the preventive and therapeutic effects of Ginkgo biloba extract(GbE) on early experimental diabetic nephropathy (DN) in ***:After anearly DN model was induced by streptozotocin,rats were administered GbE at 3doses for 12 *** blood glucose,creatinine (Cr),blood urea nitrogen(BUN),urine protein,kidney index,anti-oxidase,advanced glycosylation end prod-ucts (AGE),collagen IV and laminin,matrix metalloproteinases-2 (MMP-2) and thetissue inhibitor of metalloproteinase-2 (TIMP-2),connective tissue growth factor(CTGF),and transforming growth factor-β1 (TGF-β1) mRNA were measured bydifferent *** ultrastructural morphology and the thickness of glomeru-lar base membrane (GBM) were observed by a transmission electron ***:For the GbE-treated DN rats,when compared with the vehicle-treatedDN rats,the fasting blood glucose level,Cr,BUN,urine protein level,and theintensity of oxidative stress were significantly *** expression of MMP-2 greatly increased,and TIMP-2 ***,AGE,either in serum or in renal,the collagen IV,laminin,CTGF levels,and TGF-β1 mRNA were ***,both relative grades of mesangium hyperplasia by microscopicalobservation and the thickness of GBM by electron microscope measurement de-creased ***:GbE has protective effects on several pharma-cological targets in the progress of DN and is a potential drug for the preventionof early DN.

关键词： Ginkgo biloba extract diabetic nephropathy transforming growth factor-131 matrix metalloproteinases-2 connective tissue growth factor

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Danhong injection A modulator for Golgi structural stability after cerebral ischemiareperfusion injury

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Neural Regeneration Research 2013年第25期8卷 2343-2349页

作者： Yan Wang Zhiping Hu Wei Lu Department of Neurology Second Xiangya HospitalCentral South University

The cerebral ischemia-reperfusion model was established using the suture occlusion method, and rats were intraperitoneally given 8 mL/kg Danhong injection once a day prior to model establishment Rat brain tissues were harvested at 6, 24, 48, 72 hours after reperfusion. Immunohistochemical staining showed that transforming growth factor-J31 expression increased, while Golgi matrix protein GM130 expression decreased after cerebral ischemia-reperfusion. Danhong injection was shown to significantly up-regulate the expression of transforming growth factor-131 and GM130, and expres- sion levels peaked at 7 days after reperfusion. At 7 days after cerebral ischemia-reperfusion, Golgi morphology was damaged in untreated rats, while Golgi morphology breakage was not observed after intervention with Danhong injection. These experimental findings indicate that Danhong injec- tion can up-regulate the expression of transforming growth factor-131 and GM130, and maintain Golgi stability, thus playing a neuroprotective role in rats after cerebral ischemia-reperfusion.

关键词： neural regeneration traditional Chinese medicine Danhong injection cerebral ischemia-reperfu-sion transforming growth factor-131 GM130 Goigi matrix protein brain injury neuroprotection grants-supported paper neuroregeneration

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