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Chemical constituents from acid hydrolyzates of Panax quinquefolius total saponins and their inhibition activity to α-glycosidase and protein tyrosine phosphatase 1B

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Chinese Herbal Medicines 2020年第2期12卷 195-199页

作者： Si-wen Han Sheng-ming Shi Yu-xiao Zou Zhi-cheng Wang Yan-qun Wang Lin Shi Ting-cai Yan College of Food Science Shenyang Agricultural UniversityShenyang 110866China Tianjin Press of Chinese Herbal Medicines Tianjin Institute of Pharmaceutical ResearchTianjin 300462China Dalian Institute for Drug Control Dalian 116021China Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning Province Shenyang Agricultural UniversityShenyang 110866China

Objective:To investigate the hypoglycemic components from the acid hydrolyzates of Panax quinquefolius total saponins,and screen the active compounds by in vitro inhibitory activities toα-glycosidase enzymes and protein tyrosine phosphatase-1 B(PTP1 B).Methods:The hydrolyzates were chromatographed repeatedly over silica gel column,and the structures of the compounds were determined by means of *** in vitro bioassay was performed through the inhibitory effects onα-glucosidase or/and PTP1 ***:Eight compounds were isolated,which identified as 20(S)-panaxadiol(1),(20 S,24 R)-dammarane-20,24-epoxy-3β,6α,12β,25-tetraol(2),20(R)-dammarane-3β,12β,20,25-tetraol(3),20(S)-dammarane-3β,6α,12β,20,25-pentol(4),20(R)-dammarane-3β,12β,20,25-tetrahydroxy-3β-O-β-D-glucopyranoside(5),β-sitosterol(6),oleanolic acid(7)and 20(S)-protopanaxadiol(8).Compound 5 was ginseng triterpenoid isolated from the acid hydrolysates of total saponins from *** for the first *** this paper,the possible in vitro inhibitory activities were *** 5 exhibited significantly inhibitory activity againstα-glucosidase,and the IC50 value[(0.22±0.21)μmol/L]was about 43-fold lower than positive *** the PTP1 B inhibition assay,compound 5 indicated the strongest inhibitory effect with IC50 of(5.91±0.38)μmol/L,followed by compound 4 with IC50 of(6.21±0.21)μmol/L,which were all showed competitive inhibitory pattern by using a Lineweaver-Burk ***:These results supported the potential application of dammaranes from acid hydrolyzates of *** total saponins can be used as ingredients of ancillary anti-diabetic agent or functional factor.

关键词： Panax quinquefolius L total saponins acid hydrolysis hypoglycemic effect

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Preliminary Identification of Red Pigment and Positive Correlation between the Contents of Red Pigment and total saponins of Panax notoginseng Fruits

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Agricultural Science & Technology 2012年第9期13卷 1891-1895页

作者：赵昶灵陈中坚陈文龙支伟特魏富刚宋德功云南农业大学农学与生物技术学院云南昆明650201 文山县苗乡三七实业有限公司云南文山663000

[Objective] This study aimed to identify red pigment of Panax notoginseng fruits and explore the correlation between pigment content and total saponins of the fruits. [Method] The red pigment of Panax notoginseng fruits was preliminarily identi- fied with specific color reactions and UV-vis spectra, and the contents of the pigment and total saponins were determined via spectrophotometry. [Result] The red hues of the fruits were contributed by anthocyanins and/or the anthocyanidins. The contents of anthocyanins and total saponins of the fruits both decreased along with thinning of the red hues. The content difference of the anthocyanins in fruits with different red hues reached extremely significant level, but that of total saponins just reached significant level. [Conclusion] The red pigment of P. notoginseng fruits is anthocyanins which are of extremely significant positive correlation with total saponins in contents.

关键词： Panax notoginseng fruits Red pigment Anthocyanins total saponins Content correlation

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Screening and functional evaluation of the glucose-lowering active compounds of total saponins of Baibiandou(Lablab Semen Album)

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Digital Chinese Medicine 2021年第3期4卷 229-240页

作者： HAN Jun ZHENG Qinfang FANG Liangzi HUANG Xiaolong National and Local Joint Engineering Research Center for Key Technology of Chinese Medicinal Composition Granules Beijing Tcmages Pharmaceutical Co.Ltd.Beijing 101301China Science and Technology Innovation Center Hunan University of Chinese MedicineChangshaHunan 410208China Key Laboratory of Dong Medical Research of Hunan Province Hunan University of MedicineHuaihuaHunan 418000China Analysis and Testing Center of Hope Biotechnology Co. Ltd.WuxiJiangsu 214000China

Objective To screen forα-glucosidase inhibitor active compounds in the total saponins of Baibiandou(Lablab Semen Album)based on UHPLC-Q-Exactive Orbitrap MS technology and to evaluate its hypoglycemic activity in *** Acarbose was used as the positive control,and the median inhibitory concentration(IC50)was used as the evaluation index ofα-glucosidase inhibitory activity to establish an in vitroα-glucosidase inhibition ***,UHPLC-Q-Exactive Orbitrap MS technology was used to screen and identify the active compounds ofα-glucosidase inhibitors in the total saponins of Baibiandou(Lablab Semen Album)in order to further verify the activity of the main active monomer and to perform homologous modeling and molecular docking of yeast-derivedα-glucosidase and human-derivedα-glucosidase,while the hypoglycemic activity was evaluated in diabetic *** This study successfully identified 15 compounds with potentialα-glucosidase inhibitory activity,including Chikusetsusaponin IVa,from the total saponins of Baibiandou(Lablab Semen Album).Simultaneously,we verified the activity of the main active monomer Chikusetsusaponin IVa,and showed that it has strongα-glucosidase inhibitory ***α-glucosidase inhibitory concentration IC50 was(565.2±1.026)μg/m L,and the IC50 of acarbose,which was lower than the positive control,was(706.6±1.058)μg/m *** docking energies of Chikusetsusaponin IVa were–6.1 and–7.7 kcal/mol with yeast-derivedα-glucosidase and human-derivedα-glucosidase molecules,*** showed strong binding activity,and the levels of alanine aminotransaminase(ALT),aspartate aminotransaminase(AST),UREA,Creatinine(CREA),and cholesterol(CHO)were significantly decreased by Chikusetsusaponin IVa(Ptotal saponins of Baibiandou(Lablab Seme

关键词： Baibiandou(Lablab Semen Album) total saponins UHPLC-Q-Exactive Orbitrap MS α-Glucosidase Molecular docking Type 2 diabetic mice Chikusetsusaponin IVa

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Rubus Parvifolius *** the Growth of Leukemia K562 Cells in Vitro and in Vivo

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Chinese Journal of Integrative Medicine 2014年第1期20卷 36-42页

作者：张学进许晓峰高瑞兰许建峰 Division of Hematology Hangzhou Red Cross Hospital Institute of Hematology Chinese Medicine Hospital of Zhejiang Province Arkansas Biosciences Institute College of Agriculture and TechnologyArkansas State University

Objective：To determine the antiproliferative activity of Rubus parvifolius L.（RP）extract,its medicinal serum and RP total saponins（RPTS）against K562 cells in vitro and in ***：Nude mice models bearing leukemia tumors were treated with different concentrations of RP *** size,weight and histopathological change of leukemic tumors were ***-solid agar culture and methylthiazolyl tetrazolium（MTT）assay were used to determine in vitro the inhibition of colony formation and proliferation of K562 cells respectively by different concentrations of RP medicinal serum and ***：RP extract had a tumor inhibition rate of 84.8%when administered to mice at a dose of 1.0 g/day of crude RP root ***-solid agar culture of K562cells in the presence of 20%（v/v）of RP medicinal serum and 150 mg/L RPTS demonstrated a 50.8%and 100%inhibition of the colony forming unit（CFU）-K562,*** same doses of RP medicinal serum and RPTS showed a proliferation inhibition of 31.4%and 86.3%,respectively against K562 cells in MTT ***：RP extract and RPTS show effective antiproliferative activity against myeloid leukemia cells in vitro and in vivo.

关键词： Rubus parvifolius L. medicinal serum total saponins leukemic animal model K562 cell inhibition Chinese medicine

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Application of Multivariate Analysis Method in Evaluation of Quality and Traits of Sichuan Ophiopogon japonicus

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Medicinal Plant 2018年第4期9卷 59-61,64页

作者： Kunlun LI Xingguo ZHANG Shiming PENG Tong LI Dezhu LI School of Life Science and Engineering Southwest Jiaotong University

[Objectives] To study the relationship between the yield and total saponin content of Sichuan Ophiopogon japonicus. [Methods]The multi-indicator function model of Sichuan O. japonicus was established by using the multivariate analysis method and taking the yield of and total saponin content of Sichuan O. japonicus as indicators. [Results] In the multivariate non-linear fitting,the Pearson correlation test was used to reduce the dimension of the model,and the significant correlated variables were rejected,leaving three independent variables: the total fresh weight of the plant( x_1),the fresh weight of the aboveground part(x_2) and the fresh weight of fibrous roots(x_3),established the total saponin( y) function model for Sichuan O. japonicus: y = a_1+a_2x_2+ a_3x_3+ a_4x_1x_2+ a_5x_1x_3+ a_6x_2x_3+ a_7x_1~2+ a_8x_2~2+ a_9x_3~2.[Conclusions]When the total fresh weight of plant,fresh weight of aboveground part and fresh weight of fibrous roots were known in Sichuan O. japonicus root tuber,the total saponin content could be estimated by polynomial of these three variables. The establishment of this functional model system is expected to provide a scientific basis for the scientific prediction of the yield and total saponin content of Sichuan O. japonicus.

关键词： Sichuan Ophiopogon japonicus Biological traits total saponins Fitting analysis

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Changes in Active Components before and after Microorganism Fermentation of Rhizoma Paridis(Chonglou)

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Medicinal Plant 2018年第4期9卷 62-64页

作者： Dingqiao CHEN Ye ZHAO Zijiang YANG Zhifeng ZHANG College of Pharmacy Southwest Minzu University

[Objectives] To study the changes in active components before and after microorganism fermentation of Rhizoma Paridis( Chonglou). [Methods]Analytical methods using total saponins and total flavonoids as indicators were established. UV spectrophotometry was used to determine the changes in active components before and after fermentation of Rhizoma Paridis. [Results] The content of total saponins decreased after fermentation,while the content of total flavonoids increased. [Conclusions]The content of total saponins decreased after fermentation and the content of total flavonoids increased. The fermentation process of Monascus purpureus Went. needs further optimization.

关键词： Active components Paris polyphylla Microorganism fermentation total saponins total flavonoids

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