Screening and functional evaluation of the glucose-lowering active compounds of total saponins of Baibiandou(Lablab Semen Album)

作     者：HAN Jun ZHENG Qinfang FANG Liangzi HUANG Xiaolong 韩君;郑钦方;方良子;黄小龙

作者机构：National and Local Joint Engineering Research Center for Key Technology of Chinese Medicinal Composition GranulesBeijing Tcmages Pharmaceutical Co.Ltd.Beijing 101301China Science and Technology Innovation CenterHunan University of Chinese MedicineChangshaHunan 410208China Key Laboratory of Dong Medical Research of Hunan ProvinceHunan University of MedicineHuaihuaHunan 418000China Analysis and Testing Center of Hope Biotechnology Co.Ltd.WuxiJiangsu 214000China 

出 版 物：《Digital Chinese Medicine》 (数字中医药（英文）)

年 卷 期：2021年第4卷第3期

页      面：229-240页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 0812[工学-计算机科学与技术（可授工学、理学学位）] 100602[医学-中西医结合临床] 10[医学] 

基　　金：funding support from the Program of the Educational Commission of Hunan Province of China(No.20B418) 

主　　题：Baibiandou(Lablab Semen Album) Total saponins UHPLC-Q-Exactive Orbitrap MS α-Glucosidase Molecular docking Type 2 diabetic mice Chikusetsusaponin IVa 

摘      要：Objective To screen forα-glucosidase inhibitor active compounds in the total saponins of Baibiandou(Lablab Semen Album)based on UHPLC-Q-Exactive Orbitrap MS technology and to evaluate its hypoglycemic activity in *** Acarbose was used as the positive control,and the median inhibitory concentration(IC50)was used as the evaluation index ofα-glucosidase inhibitory activity to establish an in vitroα-glucosidase inhibition ***,UHPLC-Q-Exactive Orbitrap MS technology was used to screen and identify the active compounds ofα-glucosidase inhibitors in the total saponins of Baibiandou(Lablab Semen Album)in order to further verify the activity of the main active monomer and to perform homologous modeling and molecular docking of yeast-derivedα-glucosidase and human-derivedα-glucosidase,while the hypoglycemic activity was evaluated in diabetic *** This study successfully identified 15 compounds with potentialα-glucosidase inhibitory activity,including Chikusetsusaponin IVa,from the total saponins of Baibiandou(Lablab Semen Album).Simultaneously,we verified the activity of the main active monomer Chikusetsusaponin IVa,and showed that it has strongα-glucosidase inhibitory ***α-glucosidase inhibitory concentration IC50 was(565.2±1.026)μg/m L,and the IC50 of acarbose,which was lower than the positive control,was(706.6±1.058)μg/m *** docking energies of Chikusetsusaponin IVa were–6.1 and–7.7 kcal/mol with yeast-derivedα-glucosidase and human-derivedα-glucosidase molecules,*** showed strong binding activity,and the levels of alanine aminotransaminase(ALT),aspartate aminotransaminase(AST),UREA,Creatinine(CREA),and cholesterol(CHO)were significantly decreased by Chikusetsusaponin IVa(P0.05).In addition,it could repair damaged liver and pancreas cells of diabetic mice to some *** This study provides a basis for screeningα-glucosidase inhibitors and structural modifications of the total saponins of Baibiandou(Lablab Seme