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Changes of Relevant Indexes of Photosynthetic Pigment and Nitrogen Metabolism in Leaves of Chrysanthemum morifolium Ramat. ‘Huaibai' after TAV-free

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Plant Diseases and Pests 2016年第4期7卷 6-9,15页

作者： Zhao Xiting Liu Ke Zhu Yuting Wang Zhaoyang Zhou Yingyuan Fan Zijian Jiang Liwei Li Mingjun College of Life Science Henan Normal University Xinxiang 453007 China Henan Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs Xinxiang 453007 China Henan Engineering Laboratory of Green Crude Drugs Bioteehnology Xinxiang 453007 China Nanzhao Development Office of Chinese Crude Drugs Nanyang 474650 China

[ Objective ] The paper was to explore effects of tomato aspermy virus （TAV） -free on chlorophyll content and nitrogen metabolism of Chrysanthemum morifolium Ramat. ＇Huaibai＇, in order to provide a theoretical basis for field popularization and application of TAV-free ＇ Huai bai＇ plantlets. [ Method] With previously acquired TAV-free test-tube plantlets of ＇ Huaibai＇ as the experimental materials and its ordinary test-tube plantlets as the control （CK）, chlorophyll content and some indexes related to nitrogen metabolism in leaves were determined during subculture. [ Result] During subculture, the contents of chlorophyll a and chlorophyll b in leaves of CK and TAV-free ＇ Huaibai＇ test-tube plantlets increased with the subculture days. Both realized the largest increase at the 22＇h day of subculture, and their increase amplitudes were 5.24% and 41.69%, respectively. Compared with CK, the chlorophyll a and chlorophyll b contents of TAV-free test-tube plantlets increased at the same time point of subculture, with significant difference （P 〈0.05） or extremely significant difference （P 〈0.01 ）. Further- more, the nitrate reductase （NR） activity, free proline and soluble protein contents of TAV-free ＇ Huaibai＇ were all significantly higher than those of CK, and the highest increase amplitudes were 27.99% （ subculture for 22 d）, 28.10% （ subculture for 22 d） and 5.89% （ subculture for 38 d）, respectively. [ Conclusion] TAV-free could effectively improve chlorophyll content and nitrogen metabolism level of ＇ Huaibai＇ , and was conducive to its growth and development.

关键词： Chrysanthemum murifolium Ramat. ' Huaibai' tomato aspermy virus virus-free Chlorophyll content Nitrogen metabolism

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Effect of TAV-free on physiology of Chrysanthemum morifolium Ramat.'Huaibai'

Effect of TAV-free on physiology of Chrysanthemum morifolium...

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中国植物病理学会2015年学术年会

作者：赵喜亭 TIAN Ying ZHU Yu-ting JIANG Li-Wei LI Ming-jun College of life science Henan normal university Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs of Henan Province University Henan Province Engineering Laboratory of Green Medicinal Plant Biotechnology

To explore the effect of tomato aspermyvirus(TAV)-free on physiology of Chrysanthemum morifolium Ramat.’ Huaibai’.TAV-free ’ Huaibai’ test-tube plantlets were used as experimental materials in this *** results showed that;(1) TAV-free didn’ t change the trend of photosynthetic indexes(the chlorophyll a,chlorophyll b and total chlorophyll contents) and N metabolism related indexes(the NR activity,Proline and soluble protein contents) of during subculture,but significantly increased the chlorophyll a contents,chlorophyll b contents and total chlorophyll contents at the same time,significantly increased the NR activity,Proline and soluble protein contents,so TAV-free improved photosynthetic capacity and N metabolism level.(2) TAV-free didn’ t change the trend of lipid peroxidation related indexes(O · produce rate,the MDA and HO contents) and protection system related indexes(protective enzymes SOD,POD,CAT,GR and APX activities;antioxidants ASA and GSH contents) during subculture,but significantly reduced the absolute value of lipid peroxidation related indexes and significantly increased activities of protective enzyme and the contents of antioxidants at the same time of *** TAV-free reduced the active oxygen metabolism and improved its oxidation resistance.

关键词： Chrysanthemum morifolium Ramat.’Huaibai’ tomato aspermy virus virus-free genetic stability physiology

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