Changes of Relevant Indexes of Photosynthetic Pigment and Nitrogen Metabolism in Leaves of Chrysanthemum morifolium Ramat. ‘Huaibai' after TAV-free
Changes of Relevant Indexes of Photosynthetic Pigment and Nitrogen Metabolism in Leaves of Chrysanthemum morifolium Ramat. ‘Huaibai' after TAV-free作者机构：College of Life Science Henan Normal University Xinxiang 453007 China Henan Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs Xinxiang 453007 China Henan Engineering Laboratory of Green Crude Drugs Bioteehnology Xinxiang 453007 China Nanzhao Development Office of Chinese Crude Drugs Nanyang 474650 China
出 版 物：《Plant Diseases and Pests》 (植物病虫害研究（英文版）)
年 卷 期：2016年第7卷第4期
页 面：6-9,15页
学科分类：090706[农学-园林植物与观赏园艺] 0907[农学-林学] 09[农学]
基 金：Supported by National Natural Science Foundation of China(31372105) Subproject of Special Research of National Traditional Chinese Medicine Industry(201407005-08) Support Project of Science and Technology Innovation Teams in Colleges and Universities of Henan Province(15IRTSTHN020) Project for Construction of Innovative Science and Technology Talent Team in Henan Province(C20130037)
主 题：Chrysanthemum murifolium Ramat. ' Huaibai' Tomato aspermy virus Virus-free Chlorophyll content Nitrogen metabolism
摘 要：[ Objective ] The paper was to explore effects of Tomato aspermy virus （TAV） -free on chlorophyll content and nitrogen metabolism of Chrysanthemum morifolium Ramat. ＇Huaibai＇, in order to provide a theoretical basis for field popularization and application of TAV-free ＇ Huai bai＇ plantlets. [ Method] With previously acquired TAV-free test-tube plantlets of ＇ Huaibai＇ as the experimental materials and its ordinary test-tube plantlets as the control （CK）, chlorophyll content and some indexes related to nitrogen metabolism in leaves were determined during subculture. [ Result] During subculture, the contents of chlorophyll a and chlorophyll b in leaves of CK and TAV-free ＇ Huaibai＇ test-tube plantlets increased with the subculture days. Both realized the largest increase at the 22＇h day of subculture, and their increase amplitudes were 5.24% and 41.69%, respectively. Compared with CK, the chlorophyll a and chlorophyll b contents of TAV-free test-tube plantlets increased at the same time point of subculture, with significant difference （P 〈0.05） or extremely significant difference （P 〈0.01 ）. Further- more, the nitrate reductase （NR） activity, free proline and soluble protein contents of TAV-free ＇ Huaibai＇ were all significantly higher than those of CK, and the highest increase amplitudes were 27.99% （ subculture for 22 d）, 28.10% （ subculture for 22 d） and 5.89% （ subculture for 38 d）, respectively. [ Conclusion] TAV-free could effectively improve chlorophyll content and nitrogen metabolism level of ＇ Huaibai＇ , and was conducive to its growth and development.
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