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Electropolymerized poly(toluidine blue)-modified carbon felt for highly sensitive amperometric determination of NADH in flow injection analysis

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Journal of Environmental Sciences 2011年第6期23卷 1050-1056页

作者： Yasushi Hasebe Yue Wang Kazuya Fukuoka Department of Life Science and Green Chemistry Saitama Institute of Technology 1690 Fusaiji Fukaya Saitama 369-0293 Japan Department of Materials Science and Engineering Graduate School of Engineering Saitama Institute of Technlogy 1690 Fusaiji Fukaya Saitama 369-0293 Japan School of Chemical Engineering University of Sciences and Technology Anshan 114044 China

Poly（pheniothiazine） films were prepared on a porous carbon felt（CF） electrode surface by an electrooxidative polymerization of three phenothiazine derivatives（i.e.,Tthionine（TN）,toluidine blue（TB） and Methylene blue（MB）） from 0.1 mol/L phosphate buffer solution（pH 7.0）.Among the three phenothiazies,the poly（TB） film-modified CF exhibited an excellent electrocatalytic activity for the oxidation of nicotinamide adenine dinucleotide reduced form（NADH） at ＋0.2 V ***/*** poly（TB） film-modified CF was successfully used as working electrode unit of highly sensitive amperometric flow-through detector for *** peak currents（peak heights） were almost unchanged,irrespective of a carrier flow rate ranging from 2.0 to 4.1 mL/min,resulting in the measurement of NADH（ca.30 samples/hr） at 4.1 mL/*** peak current responses of NADH showed linear relationship over the concentration range from 1 to 30 μmol/L（sensitivity：0.318 μA/（μmol/L）;correlation coefficient：0.997）.The lower detection limit was found to be 0.3 μmol/L（S/N = 3）.

关键词： poly(phenothiazine) toluidine blue electropolymerization carbon felt NADH flow injection analysis

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Assessment of Spermatic Chromatin Decondensation by the toluidine blue Assay in Infertile Patients in Cotonou

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Advances in Reproductive Sciences 2022年第2期10卷 37-48页

作者： Yaude Alfred Azonbakin Simon Aweh Bruno Dangbemey Patrice Sissoko Sidi Boula Dossou Jean-Paul Flore Gangbo Laleye Anatole Laboratory of Histology Reproductive Biology Cytogenetics and Medical Genetics Faculty of Health Sciences Cotonou Benin Department of Gynaecology-Obstetrics Mother and Child Teaching Hospital of Cotonou Cotonou Benin Pathological Anatomy and Cytology Department of the point’s G Teaching Hospital in Bamako Mali Laboratory of Andrology Human Reproduction and Demography Research Center CERRHUD Cotonou Benin

Background: A high level of sperm chromatin decondensation results in loss of DNA quality and it is associated with poor embryonic prognosis in Assisted Reproductive Technology (ART). This had lead to introduction of sperm DNA compaction testing in assessment of clinical male infertility. toluidine blue assay is one of most commonly used tests to measure chromatin decondensation in research studies and also in clinical routine use. So the objective of our study was to assess the level of sperm chromatin decondensation by toluidine blue assay in men dealing with couple infertility. Methods: The sperm samples of 96 patients referred for infertility, over a 4-month period from September 2020 to January 2021, were stained with toluidine blue and observed under an optical microscope. These were evaluated according to the World Health Organization. Results: The 25 - 34 age group was the most represented among infertile men. There was a non-significant correlation between age and the level of spermatic chromatin condensation (r = 0.0348). Spermatic parameters had averages above standards norms, apart from that of low vitality. Spermatic chromatin decondenization affected 96% of infertile patients. Asthenozoospermia was the most common abnormality. Among standard parameters of spermogram, only the concentration of spermatozoa in the semen was weakly correlated (r = 0.18) with sperm chromatin decondensation. Conclusion: The level of sperm chromatin condensation is, on the one hand, a parameter independent of the age of the subject; on the other hand, allows to evaluate male infertility, like the classic parameters of the spermogram and spermocytogram. However, in view of the high probability of changes in the nuclear quality of sperm intrinsic to the analysis process and sperm concentration, an improvement of the toluidine blue test using the swim-up technique and associated with image processing software is possible.

关键词： Male Infertility Decondensation Spermatic Chromatin toluidine blue

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The Photopolymerization Kinetics of Acryl amide Sensitized with toluidine blue-Alcoholamine Induced by He-Ne Laser

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Chemical Research in Chinese Universities 1993年第4期9卷 329-334页

作者： FENG Min-hui, HE Wei-qiang, HE Yi and LIANG Zhao-xi (Institute of Polymer Science, Zhongshan University, Guangzhou, 510275)

toluidine blue(TB) is a good sensitizer for the polymerization of acrylnmide(AM) induced by He-Ne laser. With TB as a sensitizer and various alcoholamine as donors, the photoredox of TB and the polymerization kinetics of AM induced by He-Ne laser were investigated. It was found that either for the photoredox of TB or for the polymerization of AM, the activity of alcoholamine increases in the order of EOA

关键词： He-Ne laser Photopolymerization Acryiamide toluidine blue Alcoho-lamine

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Effects of Different toluidine blue Dyes on Presenting Chicken Intestine Mast Cells

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Animal Husbandry and Feed Science 2015年第5期7卷 301-302,313页

作者： Xu Sheng Jia Shengjun Xiong Xilong College of Veterinary Medicine Yangzhou University

Chicken intestinal tissues were fixed with 4% paraformaldehyde and stained with the dyes of 1% toluidine blue, 0.5 % toluidine blue-ethanol and toluidine blue-potassium permanganate respectively. Chicken intestinal tissues stained by 1% toluidine blue showed that mast cells presented the lavender, while the staining was light and the structure was susceptible to be intervened, and it was difficult to observe the structures of mast cells. Chicken intestinal tissues stained by toluidine blue-potassium permanganate showed that the tissue sections presented the navy blue, the structures of mast cells could not be observed clearly. Chicken intestinal tissues stained by 0.5% toluidine blue-ethanol showed that the mast cells presented the blue-violet and the outlines were clear; the cytoplasm was hy- perchromatic and the staining of nucleus was light; the structures of mast cells could be clearly distinguished from the surrounding structures. In conclusion, the staining effect of 0.5% toluidine blue-ethanol on chicken intestine mast cells was good and the structure of mast cell could be observed clearly.

关键词： Chicken toluidine blue Mast cells

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