Effects of Different Toluidine Blue Dyes on Presenting Chicken Intestine Mast Cells

作     者：Xu Sheng Jia Shengjun Xiong Xilong 

作者机构：College of Veterinary MedicineYangzhou University 

出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)

年 卷 期：2015年第7卷第5期

页      面：301-302,313页

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by the Project of Priority Academic Program Development of Jiangsu Higher Education Institutions 

主　　题：Chicken Toluidine blue Mast cells 

摘      要：Chicken intestinal tissues were fixed with 4% paraformaldehyde and stained with the dyes of 1% toluidine blue, 0.5 % toluidine blue-ethanol and toluidine blue-potassium permanganate respectively. Chicken intestinal tissues stained by 1% toluidine blue showed that mast cells presented the lavender, while the staining was light and the structure was susceptible to be intervened, and it was difficult to observe the structures of mast cells. Chicken intestinal tissues stained by toluidine blue-potassium permanganate showed that the tissue sections presented the navy blue, the structures of mast cells could not be observed clearly. Chicken intestinal tissues stained by 0.5% toluidine blue-ethanol showed that the mast cells presented the blue-violet and the outlines were clear; the cytoplasm was hy- perchromatic and the staining of nucleus was light; the structures of mast cells could be clearly distinguished from the surrounding structures. In conclusion, the staining effect of 0.5% toluidine blue-ethanol on chicken intestine mast cells was good and the structure of mast cell could be observed clearly.