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Identification of trpm7 channels in human intestinal interstitial cells of Cajal

World Journal of Gastroenterology 2009年第46期15卷 5799-5804页

作者： Byung Joo Kim Kyu Joo Park Hyung Woo Kim Seok Choi Jae Yeoul Jun In Youb Chang Ju-Hong Jeon Insuk So Seon Jeong Kim School of Korean Medicine Pusan National University Department of Surgery College of MedicineSeoul National University Department of Physiology College of MedicineChosun University Department of Anatomy College of MedicineChosun University Center for Bio-Artif icial Muscle and Department of Physiology College of MedicineSeoul National University Center for Bio-Artifi cial Muscle and Depart-ment of Biomedical Engineering Hanyang University

AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （trpm7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify trpm7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, trpm7 channel blockers, inhibited the slowwaves. Also, trpm7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that trpm7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.

关键词： Electrophysiology Interstitial cells of Cajal trpm cation channels Transient receptor potential melastatin-type 7 protein Human Gastrointestinal tract

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NGF-induced reduction of an outward-rectifying trpm7-like current in rat CA1 hippocampal neurons

NGF-induced reduction of an outward-rectifying TRPM7-like cu...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Shun-Lian TIAN,Hui JIANG,Yan ZENG,Ling-Li LI,Jing SHI~* Department of Neurobiology,Tongji Medical College,Huazhong Science and Technology University,Wuhan 430030, China

＜正＞Transient receptor potential melastatin 7（trpm7） channels are widely expressed in the nervous system;however,their function and regulation are largely *** study aimed to explore whether the current mediated by trpm7-like channels in rat CA1 hippocampal neurons could be modulated by nerve growth factor（NGF）.Using whole-cell patch clamp techniques,we identified an outward-rectifying trpm7-like current in hippocampal neurons freshly isolated from postnatal（10-day-old） *** outward component of this current was reversibly reduced by NGF in dose- and time-dependent manners,and this effect was substantially blocked by K252a,an inhibitor of *** addition,NGF-induced reduction of the trpm7-like current was abolished by U73122,a phopholipase C *** light of the abundance of NGF in hippocampus that express both TrkA and trpm7,these results suggest that the function of trpm7-like channels in hippocampal neurons may be regulated by NGF.

关键词： nerve growth factor trpm cation channels hippocampus TrkA rat patch clamp

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