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数码 2006年第8期 158-163页

作者：伍笑（摄影）

落后、土气的台式电脑会让你的桌面黯然失色．对自己好一点，添置一台个性化十足的时尚台式电脑吧，老朽们快快让道。

关键词： Desktop terminator 桌面台式电脑个性化

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terminator摩托车

摩托车 2000年第7期 25-25页

作者：鲁厚

本期封面展示的车辆是设在美国加利福尼亚南部的意大利Pro Italia Motor工厂制造的最新型摩托车,车名叫terminator。该工厂是专门生产欧洲款式定制车的企业,近年来由于高级管理层人事变动,一直没有惊世之作,新近推出的这款terminator车... 详细信息

本期封面展示的车辆是设在美国加利福尼亚南部的意大利Pro Italia Motor工厂制造的最新型摩托车,车名叫terminator。该工厂是专门生产欧洲款式定制车的企业,近年来由于高级管理层人事变动,一直没有惊世之作,新近推出的这款terminator车算是一个新热点。该车设计者为著名英国设计师John Keogh。这是专为美国市场创作的怪异车型,因此发动机依美式车

关键词：摩托车 terminator 型式

A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae

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Synthetic and Systems Biotechnology 2022年第2期7卷 657-663页

作者： Vasil D’Ambrosio Lea G.Hansen Jie Zhang Emil D.Jensen Dushica Arsovska Marcos Laloux Tadas Jakociunas Pernille Hjort Davide De Lucrezia Serena Marletta Jay D.Keasling Michael K.Jensen Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKgs.LyngbyDenmark Explora Biotech Srl Italy Joint BioEnergy Institute EmeryvilleCAUSA Biological Systems and Engineering Division Lawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Chemical and Biomolecular Engineering Department of BioengineeringUniversity of CaliforniaBerkeleyCAUSA Center for Synthetic Biochemistry Institute for Synthetic BiologyShenzhen Institutes of Advanced TechnologiesShenzhenChina

The synthetic biology toolkit for baker’s yeast,Saccharomyces cerevisiae,includes extensive genome engineering toolkits and parts ***,with the increasing complexity of engineering tasks and versatile applications of this model eukaryote,there is a continued interest to expand and diversify the rational engineering capabilities in this chassis by FAIR(findable,accessible,interoperable,and reproducible)*** this study,we designed and characterised 41 synthetic guide RNA sequences to expand the CRISPR-based genome engineering capabilities for easy and efficient replacement of genomically encoded ***,we characterize in high temporal resolution 20 native promoters and 18 terminators using fluorescein and LUDOX CL-X as references for GFP expression and OD600 measurements,***,all data and reported analysis is provided in a publicly accessible jupyter notebook providing a tool for researchers with low-coding skills to further explore the generated data as well as a template for researchers to write their own *** expect the data,parts,and databases associated with this study to support a FAIR-compliant resource for further advancing the engineering of yeasts.

关键词： gRNA Promoter terminator Yeast Standardization

Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression

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Synthetic and Systems Biotechnology 2017年第4期2卷 295-301页

作者： Erqing Jin Lynn Wong Yun Jiao Jake Engel Benjamin Holdridge Peng Xu Department of Chemical Biochemical and Environmental EngineeringUniversity of Maryland Baltimore County1000 Hilltop CircleBaltimoreMD 21250United States Department of Food Science and Engineering Jinan University601 West Huangpu RoadGuangzhou 510632China

Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway *** the way,combinatorial gene expression control through modification of regulatory element offered tremendous opportunity for fine-tuning gene expression and generating digital-like genetic *** this report,we present an efficient evolutionary approach to build a range of regulatory control *** reported method allows for rapid construction of promoter,5'UTR,terminator and trans-activating RNA *** overlapping oligos with high portion of degenerate nucleotides flanking the regulatory element could be efficiently assembled to a vector expressing fluorescence *** approach combines high mutation rate of the synthetic DNA with the high assembly efficiency of Gibson *** constructed library demonstrates broad range of transcriptional or translational gene expression ***,both the promoter library and 50UTR library exhibits gene expression dynamics spanning across three order of *** terminator library and trans-activating RNA library displays relatively narrowed gene expression *** reported study provides a versatile toolbox for rapidly constructing a large family of prokaryotic regulatory *** libraries also facilitate the implementation of combinatorial pathway engineering principles and the engineering of more efficient microbial cell factory for various biomanufacturing applications.

关键词： Promoter 50UTR terminator Riboregulatory Trans-activating RNA Library Synthetic biology

Optimized Technology for Residuum Processing in the ARGG Unit

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China Petroleum Processing & Petrochemical Technology 2006年第2期8卷 25-31页

作者： Pan Luoqi Yuan hongxing Nie Baiqiu Chemical Industry Academy of Sichuan University Chengdu 610065 Baling Petrochemical Company SINOPEC Yueyang 414014

The influence of feedstock property on operation in the FCC unit was studied to identify the cause leading to deteriorated products distribution related with increasingly heavier feedstock for the ARGG unit. In order to maximize the economic benefits of the ARGG unit a string of measures, including the modification of catalyst formulation, retention of high catalyst activity, application of mixed termina-tion agents to control the reaction temperature and once-through operation, and optimization of catalyst regeneration technique, were adopted to adapt the ARGG unit to processing of the heavy feedstock with its carbon residue equating to 7 % on an average. The heavy oil processing technology has brought about apparent economic benefits.

关键词： heavy oil ARGG catalyst carbon residue terminator

VLF/LF Amplitude Perturbations before Tuscany Earthquakes, 2013

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Natural Science 2017年第12期9卷 437-456页

作者： Balaram Khadka Keshav Prasad Kandel Sudikshya Pant Karan Bhatta Basu Dev Ghimire St. Xavier’s College Kathmandu Nepal Nepalese Center for Research in Physical Sciences (NCRPS) Kathmandu Nepal

The US Navy VLF/LF Transmitter’s NSY signal (45.9 kHz) transmitted from Niscemi, Sicily, Italy, and received at the Kiel Long Wave Monitor, Germany, was analyzed for the period of two months, May and June (EQ-month) of 2013. There were 12 earthquakes of magnitude greater than 4 that hit Italy in these two months,?of which the earthquake of 21st June having magnitude of 5.2?and a shallow focal depth of 5 km was the major one. We studied the earthquake of 21st of June 2013, which struck Tuscany,?Central Italy, (44.1713°N and?10.2082°E) at 10:33 UT, and also analyzed the effects of this earthquake on the?sub-ionos-?pheric VLF/LF signals. In addition, we also studied another earthquake, of magnitude 4.9, which hit the same place at 14:40 UT on 30th of June and had shallow focal depth of 10 km. We assessed the data using terminator time (TT) method and night time fluctuation method and found unusual changes in VLF/LF amplitudes/phases. Analysis of trend, night time dispersion, and night time fluctuation was also carried and several anomalies were detected. Most ionospheric perturbations in these parameters were found in the month?of June, from few days to few weeks prior to the earthquakes. Moreover, we filtered the possible effects due to geomagnetic storms, auroras, and solar activities?using parameters like?Dst index, AE index, and Kp index for analyzing the geomagnetic effects,?and Bz (sigma) index, sunspot numbers, and solar index F10.7 for analyzing the solar activities for the confirmation of anomalies as precursors.

关键词： D-Layer VLF/LF terminator Times SST SRT DLPT DLDT Night Time Fluctuation

Advances in the Identification of Genetically Modified Rice with Real-time PCR and Multiplex PCR

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Agricultural Biotechnology 2017年第3期6卷 23-25,29页

作者： Juan QIU Institute of Food Crops Hubei Academy of Agricultural Sciences

In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified （GM） rice, and the controversy over the commercialization of GM rice. And the identification of GM rice is of great significance. Therefore, in the present study, the po- tential problems in the identification of GM rice with PCR were analyzed both at a technical level and from a theoretical perspective. In addition, PCR detection on the transgenic elements： promoter, terminator, internal reference gene and target gene was discussed, respectively. The possible solutions were proposed based on the principles of plant virology and genetic engineering.

关键词： Genetically modified (GM) rice Qualitative detection Promoter terminator Bt gene Multiplex PCR Real-time PCR

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光突发交换中的单向资源预留协议

光通信技术 2004年第11期28卷 11-13页

作者：黄学田叶培大张民葛振斌牛长流北京邮电大学光通信中心北京100876

介绍了光突发交换网中的单向资源预留协议,对各种协议的特点进行了比较,并以JIT协议为例,详细说明了单向资源预留和资源释放的实现方式。

关键词：光突发交换单向资源预留 just in time,just enough time In—band terminator