Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression

作     者：Erqing Jin Lynn Wong Yun Jiao Jake Engel Benjamin Holdridge Peng Xu 

作者机构：Department of ChemicalBiochemical and Environmental EngineeringUniversity of Maryland Baltimore County1000 Hilltop CircleBaltimoreMD 21250United States Department of Food Science and EngineeringJinan University601 West Huangpu RoadGuangzhou 510632China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2017年第2卷第4期

页      面：295-301页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：The authors would like to acknowledge the Department of Chemical Biochemical and Environmental Engineering College of Engineering and Information Technology Office of the Vice President for Research(gratn number 10145-1113-021-STRT7XUPMAIN)at University of Maryland Baltimore County for funding support 

主　　题：Promoter 50UTR Terminator Riboregulatory Trans-activating RNA Library Synthetic biology 

摘      要：Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway *** the way,combinatorial gene expression control through modification of regulatory element offered tremendous opportunity for fine-tuning gene expression and generating digital-like genetic *** this report,we present an efficient evolutionary approach to build a range of regulatory control *** reported method allows for rapid construction of promoter,5 UTR,terminator and trans-activating RNA *** overlapping oligos with high portion of degenerate nucleotides flanking the regulatory element could be efficiently assembled to a vector expressing fluorescence *** approach combines high mutation rate of the synthetic DNA with the high assembly efficiency of Gibson *** constructed library demonstrates broad range of transcriptional or translational gene expression ***,both the promoter library and 50UTR library exhibits gene expression dynamics spanning across three order of *** terminator library and trans-activating RNA library displays relatively narrowed gene expression *** reported study provides a versatile toolbox for rapidly constructing a large family of prokaryotic regulatory *** libraries also facilitate the implementation of combinatorial pathway engineering principles and the engineering of more efficient microbial cell factory for various biomanufacturing applications.