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Identification and molecular characterization of exosome-like vesicles derived from taenia asiatica adult worms

Identification and molecular characterization of exosome-lik...

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第十一届全国寄生虫学青年工作者学术研讨会

作者： Panhong Liang Li Mao Yanping Li Shaohua Zhang Xiaola Guo Guangxue Liu Lijie Wang Junling Hou Yadong Zheng Xuenong Luo State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteCAAS College of Life Sciences Wuhan University

taenia asiatica is one of the most important food-borne parasites and posts a great threat to human *** far little is known about a specific infection and immune escape mechanism of ***,exosome-like vesicles have been emerging as a regulator in the interactions between parasites and hosts,which provide a new sight for the research on the infection of ***,exosome-like vesicles were collected from the excretory/secretory products of cultured *** and purified by differential *** purified vesicles,ranging from 50 to 150 nm in size,were identified as exosomes by transmission electron microscope and nanoparticle tracking *** analysis showed that a total of 843 proteins were identified in the exosome-like vesicles(737 from *** and 106 from the host) and enzymes involved in metabolic processes,such as glyceraldehyde 3 phosphate dehydrogenase,fructose 1,6 bisphosphate aldolase,cytosolic malate dehydrogenase,enolase and so on,were enriched in *** abundant proteins from proteomic analysis 14-3-3 and enolase were present in the exosome-like vesicles by Immunogold labeling ***,we validated the expressions of *** mi RNAs,including 6 known mi RNAs(tas-mi R-71,tas-mi R-1,tas-mi R-7,tas-mi R-9,tas-mi R-10,tas-let-7) and 3 novel mi RNAs(tas-m0022-3 p,tas-m0816-3 p,tas-m0082-5 p).the eight mi RNAs expression levels were mainly consistent with those obtained by high-throughput ***,we also demonstrated that these exosomes were internalized by Lo Vo cells in vitro through uptake experiment of exosome-like vesicles labeled with the green fluorescent dye *** study firstly isolated and identified exosome-like vesicles derived from *** adult worms,and described molecular characteristics of some cargoes loaded on these ***,exosome-like vesicles were internalized by Lo Vo *** findings provide new insights into the interaction between

关键词： t.asiatica exosome-like vesicles proteome miRNA

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Infection of taenia asiatica in a Bai people in Dali

Infection of Taenia asiatica in a Bai people in Dali

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Li Wang Shuai Wang Xue peng Cai State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences College of Veterinary Medicine Jilin University

Objective: Human taeniasis is caused by 3 species of taeniid tapeworms, t. solium, t. saginata, and t. asiatica. In the present study, we are trying to report a human case of t. asiatica infection by genetic analyses based on the mitochondrial cytochrome c oxidase subunit 1(cox1) and the cytochrome b(cytb) genes. A patient(Bai nationality) was found to have the symptoms of taeniasis with discharged proglottids in Dali, China. Method: Genomic DNA(g DNA) was extracted from the proglottid of the tapeworm. Purified g DNA was used as a template for PCR for cox1 and cytb and the amplification products were fractionated using 1%(w/v) agarose gel, purified and then sequenced, respectively. the sequences for cox1 and cytb were aligned with the corresponding sequences of tapeworms obtained from Gen Bank database. Phylogenetic analyses were performed using the neighbor-joining(NJ) method by MEGA. Results: we observed 1620 bp nucleotide sequence from the mitochondrial cox1 gene of tapeworm identity of 99%(1 different base) with t. asiatica and 96%(65 different bases) with t. saginata, respectively;1068 bp sequence of the mitochondrial cytb gene had an identity of 99%(2 different bases) with t. asiatica and 96%(43 different bases) with t. saginata, respectively. the phylogenetic analysis revealed that the tapeworm was closely related to t. asiatica. Conclusion: Our findings support that people are caused by t. asiatica. Neither t. saginata nor t. solium were detected during the same period, so t. asiatica was considered to be the dominant species causing human taeniasis in Dali China.

关键词： t.asiatica cox1 gene cytb gene

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