Identification and molecular characterization of exosome-like vesicles derived from Taenia asiatica adult worms
作者单位：State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteCAAS College of Life SciencesWuhan University
会议名称：《第十一届全国寄生虫学青年工作者学术研讨会》
会议日期：2018年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
基 金：National Key Research and Development Program of China(2017YFD0501303) the National Natural Science Foundation of China(No.31372433)
关 键 词：T.asiatica exosome-like vesicles proteome miRNA
摘 要：Taenia asiatica is one of the most important food-borne parasites and posts a great threat to human *** far little is known about a specific infection and immune escape mechanism of ***,exosome-like vesicles have been emerging as a regulator in the interactions between parasites and hosts,which provide a new sight for the research on the infection of ***,exosome-like vesicles were collected from the excretory/secretory products of cultured *** and purified by differential *** purified vesicles,ranging from 50 to 150 nm in size,were identified as exosomes by transmission electron microscope and nanoparticle tracking *** analysis showed that a total of 843 proteins were identified in the exosome-like vesicles(737 from *** and 106 from the host) and enzymes involved in metabolic processes,such as glyceraldehyde 3 phosphate dehydrogenase,fructose 1,6 bisphosphate aldolase,cytosolic malate dehydrogenase,enolase and so on,were enriched in *** abundant proteins from proteomic analysis 14-3-3 and enolase were present in the exosome-like vesicles by Immunogold labeling ***,we validated the expressions of *** mi RNAs,including 6 known mi RNAs(tas-mi R-71,tas-mi R-1,tas-mi R-7,tas-mi R-9,tas-mi R-10,tas-let-7) and 3 novel mi RNAs(tas-m0022-3 p,tas-m0816-3 p,tas-m0082-5 p).The eight mi RNAs expression levels were mainly consistent with those obtained by high-throughput ***,we also demonstrated that these exosomes were internalized by Lo Vo cells in vitro through uptake experiment of exosome-like vesicles labeled with the green fluorescent dye *** study firstly isolated and identified exosome-like vesicles derived from *** adult worms,and described molecular characteristics of some cargoes loaded on these ***,exosome-like vesicles were internalized by Lo Vo *** findings provide new insights into the interaction between