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Dynamics and diversity of symbiotic bacteria in Apolygus lucorum at different developmental stages

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Journal of Cotton Research 2023年第1期6卷 37-47页

作者： XUE Hui ZHU Xiangzhen WANG Li ZHANG Kaixin LI Dongyang JI Jichao NIU Lin GAO Xueke LUO Junyu CUI Jinjie State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Zhengzhou Research Base State Key Laboratory of Cotton Biology/Zhengzhou UniversityZhengzhou 450001HenanChina Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory/College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070HubeiChina

Background Apolygus lucorum is a worldwide omnivorous pest damaging a range of crops and causing great economic *** bacteria living in insects play a key role in the nutrition,physiology,and behavior of ***,we present an experiment using Illumina HiSeq sequencing targeting the V3–V4 regions of bacteria’s 16S rRNA throughout the entire life cycle of *** The first and second instar nymphs have the largest alpha diversity compared with other life stages of the *** phyla Proteobacteria(72.29%),Firmicutes(15.24%),Actinobacteria(7.76%)exhibit the largest relative abundance in all developmental ***(23.97%)and Lactococcus(10.62%)are the two genera with the high-est relative *** relative abundance of Erwinia in the nymph stage is significantly greater than the adult stage,and the relative abundance of Lactococcus in 6-day-old and 9-day-old adult females is higher compared with adult *** These results reveal that microbial community composition and relative abundance shift dynamically at different life stages,implying that different bacterial phyla and genera may have specific roles in specific life stages such as metabolism,nutrition absorption,detoxification,and *** study reveals for the first time the community composition and ecological dynamics of symbiotic bacteria throughout the life stages of ***,and thus may provide insight to new strategies for pest control.

关键词： symbiotic bacteria Apolygus lucorum Life cycle Pest control Community composition Relative abundance Community richness

Effects of α-pinene on the pinewood nematode（Bursaphelenchus xylophilus） and its symbiotic bacteria

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Effects of α-pinene on the pinewood nematode（Bursaphelench...

Universally improving effect of mixed electron donors on the CO_2 fixing efficiency of non-photosynthetic microbial communities from marine environments

中国植物病理学会2019年学术年会

作者： Wang Xu Yu Yanxue Ge Jianjun Cheng Xinyue College of Life Sciences Beijing Normal University Chinese Academy of Inspection and Quarantine

The pinewood nematode（PWN）,Bursaphelenchus xylophilus,is an important plantparasitic nematode that can cause severe mortality of pine *** PWN-induced harm to plants may be closely related to the abundance and diversity of the symbiotic microorganisms of the parasitic *** this study,nematodes were divided into untreated and antibiotic-treated *** were treated by fumigation with different amounts of a-pinene,and the resultant mortality rates were analyzed *** of symbiotic bacteria were calculated as colony-forming units/*** sequencing was used to investigate the bacterial community *** results showed that the mortality of nematodes increased slightly with increasing concentration of a-pinene,and nematodes untreated with antibiotics were more sensitive to a-pinene than those treated with *** highest abundance of symbiotic bacteria was obtained via medium and low levels of a-pinene,but for which community diversity was the lowest（Shannon and Simpson indexes）.The proportion of Pseudomonas *** the symbiotic bacteria of nematodes without antibiotics was relatively high（more than 70%）,while that of Stenotrophomonas *** low（6%-20%）.However,the proportion of Stenotrophomonas *** larger than that of the Pseudomonas *** the symbiotic bacteria associated with the antibiotictreated *** *** after pinene treatment,whereas Stenotrophomonas *** results indicate that although a-pinene has low toxicity to PWNs over a short time period,α-pinene ultimately influences the abundance and community diversity of the symbiotic bacteria of these nematodes;this influence may potentially disturb the development and reproduction of nematodes in the process of infecting pine trees.

关键词： pinewood nematode(PWN) Bursaphelenchus xylophilus α-pinene symbiotic bacteria

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Journal of Environmental Sciences 2014年第8期26卷 1709-1716页

作者： Jiajun Hu Lei Wang Shiping Zhang Yuanqing Wang Fangming Jin Xiaohua Fu Huirong Li School of Environmental Science and Engineering State Key Laboratory of Pollution Control and Resource ReuseTongji University Low-Carbon Conversion Science and Engineering Center Shanghai Advanced Research InstituteChinese Academy of Sciences School of Environmental Science and Engineering Shanghai Jiao Tong University Polar Research Institute of China

The universality of improved CO2 fixing upon the addition of mixed electron donors（MEDs）composed of Na2 S,NO2-,and S2O32-to non-photosynthetic microbial communities（NPMCs）obtained from 12 locations in four oceans of the world was validated. The CO2 fixing efficiencies of NPMCs were universally enhanced by MED compared with those obtained using H2 alone as electron donor,with average increase of about 276%. An increase in microbial inoculation concentration could increase the net amount of CO2 fixing to853.34 mg/L in the presence of MED. NO2-and S2O32-may play the roles of both electron acceptor and electron donor under aerobic conditions,which may improve the energy utilization efficiency of NPMC and enhance the CO2 fixation efficiency. The sequence determination of 16 S ribosomal deoxyribonucleic acid（rDNA） from 150 bacteria of NPMC showed that more than 50% of the bacteria were symbiotic and there were many heterotrophic bacteria such as Vibrio natriegens. These results indicate that NPMC acts as a symbiotic CO2 fixing system. The interaction between autotrophic and heterotrophic bacteria may be a crucial factor supporting ladder utilization and recycling of energy/carbon source.

关键词： Non-photosynthetic microbial community Mixed electron donors CO2fixing symbiotic bacteria Heterotrophic bacteria

Diversity of bacteria in different life stages and their impact on the development and reproduction of Zeugodacus tau(Diptera:Tephritidae)

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Insect Science 2021年第2期28卷 363-376页

作者： MdShibly Noman Ge Shi Li-Jun Liu Zhi-Hong Li Department of Entomology MOA Key Lab of Pest Monitoring and Green ManagementCollege of Plant ProtectionChina Agricultural UniversityBeijingChina

Fruit flies usually harbor diverse communities of bacteria in their digestive systems,which are known to play a significant role in their ***,little information is available on Zeugodacus tau,a polyphagous pest *** study reports the first extensive analysis of bacterial communities in different life stages and their effect on the development and reproduction of laboratory-reared Z *** bacteria were identified using the conventional method and all bacteria were identified by highthroughput technologies(16S ribosomal RNA gene sequencing of V3-V4 region).A total of six bacterial phyla were identified in larvae,pupae,and male and female adult flies,which were distributed into 14 classes,32 orders,58 families and 96 *** was the most represented phylum in all the stages except ***,Klebsiella,Providencia,and Pseudomonas were identified by conventional and next-generation sequencing analysis in both male and female adult flies,and Enterobacter was found to be the main *** being fed with antibiotics from the first instar larvae,bacterial diversity changed markedly in the adult *** flies laid eggs and needed 20 days before oviposition while the treated flies showed ovary development inhibited and were not able to lay eggs,probably due to the alteration of the *** findings provide the cornerstone for unexplored research on bacterial function in Z tau,which will help to develop an environmentally friendly management technique for this kind of harmful insect.

关键词： antibiotics bacterial diversity fitness oviposition symbiotic bacteria Zeugodacus tau

A new bacteria-free strategy induced by MaGal2 facilitates pinewood nematode escape immune response from its vector beetle

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Insect Science 2021年第4期28卷 1087-1102页

作者： Chi Zhang Jacob D.Wickham Lilin Zhao Jianghua Sun State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijingChina

symbiotic microbes play a crucial role in regulating parasite–host interactions;however,the role of bacterial associates in parasite–host interactions requires *** this study,we showed that,instead of introducing numerous symbiotic bacteria,dispersal of 4th-stage juvenile(JIV)pinewood nematodes(PWNs),Bursaphelenchus xylophilus,only introduced few bacteria to its vector beetle,Monochamus alternatus(Ma).JIV showed weak binding ability to five dominant bacteria species isolated from the beetles’pupal *** was especially the case for binding to the opportunistic pathogenic species Serratia marcescens;the nematodes’bacteria binding ability at this critical stage when it infiltrates Ma for dispersal was much weaker compared with Caenorhabditis elegans,Diplogasteroides asiaticus,and propagative-stage *** associated bacterium ***,which was isolated from the beetles’pupal chambers,was unfavorable to Ma,because it caused a higher mortality rate upon injection into *** addition,*** in the tracheae caused more immune effector disorders compared with PWN ***_Galectin2(MaGal2),a pattern-recognition receptor,was up-regulated following PWN *** MaGal2 protein formed aggregates with five dominant associated bacteria in ***,MaGal2 knockdown beetles had up-regulated prophenoloxidase gene expression,increased phenoloxidase activity,and decreased PWN *** study revealed a previously unknown strategy for immune evasion of this plant pathogen inside its vector,and provides novel insights into the role of bacteria in parasite–host interactions.

关键词： galectin immune evasion pinewood nematode symbiotic bacteria vector beetle

Metabolic relationships between marine red algae and algae‑associated bacteria

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Marine Life Science & Technology 2024年第2期6卷 298-314页

作者： Kyung Hyun Kim Jeong Min Kim Ju Hye Baek Sang Eun Jeong Hocheol Kim Hwan Su Yoon Che Ok Jeon Department of Biological Sciences and Biotechnology Hannam UniversityDaejon 34054Republic of Korea Department of Life Science Chung-Ang UniversitySeoul 06974Republic of Korea Department of Biological Sciences Sungkyunkwan UniversitySuwon 16419Republic of Korea

Mutualistic interactions between marine phototrophs and associated bacteria are an important strategy for their success-ful survival in the ocean,but little is known about their metabolic ***,bacterial communities in the algal sphere(AS)and bulk solution(BS)of nine marine red algal cultures were analyzed,and Roseibium and Phycisphaera were identified significantly more abundantly in AS than in *** metabolic features of Roseibium RMAR6-6(isolated and genome-sequenced),Phycisphaera MAG 12(obtained by metagenomic sequencing),and a marine red alga,Porphyridium purpureum CCMP1328(from GenBank),were analyzed ***6-6 has the genetic capability to fix nitrogen and produce B vitamins(B1,B2,B5,B6,B9,and B12),bacterioferritin,dimethylsulfoniopropionate(DMSP),and phenylacetate that may enhance algal growth,whereas MAG 12 may have a limited metabolic capability,not producing vitamins B9 and B12,DMSP,phenylacetate,and siderophores,but with the ability to produce bacitracin,possibly modulating algal *** CCMP1328 lacks the genetic capability to fix nitrogen and produce vitamin B12,DMSP,phenylacetate,and *** was shown that the nitrogen-fixing ability of RMAR6-6 promoted the growth of ***-pureum,and DMSP reduced the oxidative stress of *** metabolic interactions between strain RMAR6-6 and *** CCMP1328 were also investigated by the transcriptomic analyses of their monoculture and *** together,potential metabolic relationships between Roseibium and *** were *** study provides a bet-ter understanding of the metabolic relationships between marine algae and algae-associated bacteria for successful growth.

关键词： Metabolic relationship Marine red algae symbiotic bacteria Roseibium Phycisphaera Porphyridium

Gut bacterium promotes host fitness in special ecological niche by affecting sugar metabolism in Drosophila suzukii

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Insect Science 2023年第6期30卷 1713-1733页

作者： Huan-Huan Gao Shan Zhao Rui-Juan Wang Dong-Yun Qin Peng Chen An-Sheng Zhang Qian-Ying Zhuang Yi-Fan Zhai Xian-Hong Zhou Institute of Plant Protection Shandong Academy of Agricultural SciencesJinanChina Shandong Academy of Grape JinanChina

As an important fruit pest of global significance,Drosophila suzukii occupies a special ecological niche,with the characteristics of high sugar and low protein *** niche differs from those occupied by other fruit-damaging Drosophila *** bacteria substantially impact the physiology and ecology of ***,the contribution of gut microbes to the fitness of *** in their special ecological niche remains *** this study,the effect of Klebsiella oxytoca on the development of *** was examined at physiological and molecular *** results showed that,after the removal of gut microbiota,the survival rate and longevity of axenic *** decreased *** of *** to the midgut of *** advanced the development level of *** differentially expressed genes and metabolites between axenic and ***-reintroduced *** were enriched in the pathways of carbohydrate *** advancement was achieved through an increased glycolysis rate and the regulation of the transcript level of key genes in the glycolysis/gluconeogenesis *** oxytoca is likely to play an important role in increasing host fitness in their high-sugar ecological niche by stimulating the glycolysis/gluconeogenesis *** a protein source,bacteria can also provide direct nutrition for ***,which depends on the quantity or biomass of *** result may provide a new target for controlling *** by inhibiting sugar metabolism through eliminating the effect of *** and thus disrupting the balance of gut microbial communities.

关键词： carbohydrate metabolism Drosophila suzuki glycolysis/gluconeogenesis Klebsiella oxytoca symbiotic bacteria

Cradle for the newborn Monochamus saltuarius:Microbial associates to ward off entomopathogens and disarm plant defense

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Insect Science 2023年第4期30卷 1165-1182页

作者： Si-Xun Ge Jia-Xing Li Zhuo-Heng Jiang Shi-Xiang Zong Li-Li Ren Bejing Key Laboratory for Forest Pest Control Bejing Forestry UniversityBeijingChina School of Science Westlake UniversityHangzhouChina Sino-French Joint Laboratory for Invasive Forest Pests in Eurasia Bejing Forestry University-French National Research Institute for AgricultureFood and Environment(INRAE)BejingChina

The Japanese pine sawyer,Monochamus saltuarius,as a beetle vector of Bur-saphelenchus xylophilus(pine wood nematode),is an economically important forest pest in *** feed on the phloem and xylem of conifers,*** needs to overcome various stress factors,including coping with entomopathogenic bacteria and also various plant secondary compounds(PSCs).As an important adaptation strategy to colonize host trees,*** deposit eggs in oviposition pits to shield their *** pits har-bor bacterial communities that are involved in the host adaptation of *** to the ***,the composition,origin,and functions of these oviposition pit bacteria are rarely *** this study,we investigated the bacterial community associated with *** oviposition pits and their ability to degrade *** showed that the bacterial community structure of *** oviposition pits significantly differed from that of uninfected ***,the oviposition pit bacteria were predicted to be enriched in PSC degradation *** microbial community also harbored a lethal strain of Serratia,which was significantly ***,metatranscriptome anal-ysis indicated that genes involved in PSCs degradation were expressed complementarily among the microbial communities of oviposition pits and *** vitro degrada-tion showed that bacteria cultured from oviposition pits degraded more monoterpenes and flavonoids than bacteria cultured from uninfected phloem *** of ovipo-sition pits increased the mortality of newly hatched larvae and resulted in a significant decrease in body weight in the early ***,our results reveal that *** construct oviposition pits that harbor a diverse microbial community,with stronger PSCs degradation abilities and a low abundance of entomopathogenic bacteria,resulting in the increased fitness of newly hatched larvae.

关键词： community structure entomopathogenic bacteria host plant adaptation plant secondary metabolites symbiotic bacteria

Population genetic structure of Sitobion miscanthi in China

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Journal of Integrative Agriculture 2022年第1期21卷 178-187页

作者： SUN Jing-xuan LI Qian TAN Xiao-ling FAN Jia ZHANG Yong QIN Yao-guo Frédéric FRANCIS CHEN Ju-lian State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Department of Functional and Evolutionary Entomology Gembloux Agro-Bio TechUniversity of LiègeGembloux 5030Belgium Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences Mudanjiang 157000P.R.China

The wheat aphid, Sitobion miscanthi, is one of the most destructive pests of wheat plants in the temperate regions of China. Little is known about the genetic structure evolution of the different geographic populations of S. miscanthi with its migration. In this study, we investigated the population genetic structure and demographic history of S. miscanthi by analysing 18 geographical populations across China using one mitochondrial gene, COI;one nuclear gene, EF-1α;and two endosymbiont Buchnera genes, gnd and trpA. Analysis of data from the various groups showed high haplotype diversity and low nucleotide variation. SAMOVA analysis did not find a correlation between genetic distance and geographic distance. However, areas with high population diversity exhibited high haplotype diversity. Therefore, we speculate that there are two main natural migration pathways of S. miscanthi in China. One is from Yunnan to the Sichuan Basin, and the other is from Wuhan, Xinyang and Jiaodong Peninsula areas to the northwest. Based on this hypothesis, we inferred that these aphid populations appear first in the southwestern and southern regions and spread to the north with the help of the southeastern and southwestern monsoons, which occur in spring and summer. In autumn, the aphids spread southward with the northeastern and northwestern monsoons.

关键词： Sitobion miscanthi molecular marker COI symbiotic bacteria phylogenetic tree